Corporate Banner
Satellite Banner
RNAi
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

High Throughput Screening of Infectious Virus Entry in Mammalian Cells

Published: Thursday, June 07, 2007
Last Updated: Thursday, June 07, 2007
Bookmark and Share
Researchers at Swiss Federal Institute of Technology are using two customized Tecan Freedom EVO® 200 liquid handling workstations to silence 7,000 mammalian genes.

Researchers at the Institute of Molecular Systems Biology (IMSB), Swiss Federal Institute of Technology (ETH), Zurich, Switzerland, are using two customized Tecan Freedom EVO® 200 liquid handling workstations as part of a study to identify the specific sets of host genes that viruses depend on to infect cells.

The large-scale, genome-wide study aims to individually silence 7,000 mammalian genes using RNA interference (RNAi), and measure how each silencing affects the abilities of 11 different viruses, including influenza, herpes and SV40, to infect mammalian hosts.

The automated set-up is critical to the project, as analyzing the effects of silencing 7,000 genes on one virus requires 207 384-well plates and takes about two weeks to complete.

One Freedom EVO workstation is equipped with a Te-MO™ 384-channel pipetting head to perform all the transfection and infection assays. The second workstation has a Te-Stack™ stacker module and two fully integrated Cell-Works microscopes for automated quantification of the proportion of virus-transfected cells.

Dr Pelkmans, assistant professor at the IMSB, explained: “The project is providing valuable information about how viruses hijack endocytic pathways for host entry and, potentially, could lead to the development of novel anti-viral therapies targeted against host cellular genes instead of viral components. We have already seen some very exciting results.”


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,500+ scientific posters on ePosters
  • More than 5,000+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Tecan Provides Automated Solutions for Molecular Pharmacology
Leibniz-Institut uses Tecan’s Freedom EVO® workstations for systematic high throughput screening of bioactive small molecule libraries.
Tuesday, July 06, 2010
Screening Center Chooses Tecan for Reliability and Support
The University of Chicago’s CSC is using Tecan’s Freedom EVO® workstations to provide high throughput cell screening services.
Friday, June 11, 2010
Tecan Platforms Improve Microarray Throughput and Research Quality in South Africa
South African researchers have established a high throughput biological research platform with a number of automated microarray processing systems from Tecan.
Friday, February 22, 2008
Tecan Boosts RNA Work in France
Tecan's Freedom EVO® Workstation has been used to develop automated in vitro selection techniques for identifying and isolating aptamers.
Wednesday, March 29, 2006
Scientific News
Developing Novel Ear Infection Treatments
Research team engineers antibiotic gel for treating middle ear infections.
Blood Pressure Drug May Boost Effectiveness of Lung Cancer Treatment
Researchers at Imperial College London have suggested that the blood pressure drug may make a type of lung cancer treatment more effective.
Wearable Microscope Can Measure Fluorescent Dyes Through Skin
UCLA research could make monitoring disease biomarkers easier and more cost-effective.
Potential of New Insect Control Traits in Agriculture
Researchers have discovered a protein that shows promise as an alternate corn rootworm control mechanism.
Fighting Cancer with Sticky Nanoparticles
Treatment that uses bioadhesive nanoparticles drug carriers proved more effective than conventional treatments for certain cancers.
Fighting Plant Pathogens with RNA
Researchers develop strategy that could lead to environmentally friendly fungicide to fight pathogens.
Smart Material Hunts Cancers
Team has created smart material that locates and images cancer or tumour sites in tissue.
Gene Therapy Technique May Help Prevent Cancer Metastasis
Gene-regulating RNA molecules could help treat early-stage breast cancer tumors before they spread.
Enhancing Antibiotics to Defeat Resistant Bacteria
Scientists enhance ability of antibiotics to defeat resistant types of bacteria using molecules called PPMOs
Examining mtDNA May Help Identify Unknown Ancestry That Influences Breast Cancer Risk
Researchers studying mtDNA in a group of triple negative breast cancer patients found that 13 percent of participants were unaware of ancestry that could influence their risk of cancer.
SELECTBIO

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,000+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!