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Functional Analysis of RAD51B and RAD23B SNPs using Circular Chromosome Conformation Capture (4C) in Human Prostate Cell Lines
C. Cieza-Borrella1, S. Wakerell1 , Q. Karlsson1 , R.A. Eeles1,2, Z. Kote-Jarai1

The aim of this study is to understand the mechanism behind the association between single nucleotide polymorphisms (SNPs), lying in non-coding regions near or within RAD51B and RAD23B, and prostate cancer risk by analysing their interactions with the whole genome using Circular Chromosome Conformation Capture (4C).

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Microfluidic extrusion of cell-laden hydrogel fibers for 3D-Bioprinting
Cristina Colosi, Laura Belloni, Giancarlo Ruocco

An overview on how the incorporation of microfluidic platforms in 3D-bioprinters can open new routes in the creation of heterogeneous, tissue-specific bio-constructs and in the high-throughput screening of bioinks compositions for regenerative medicine and in vitro tissue modeling.

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CRISPR-Cas9 genome editing utilizing chemically synthesized RNA
Michael Delaney, Kaizhang He, Eldon Chou, Amanda Haas, Žaklina Strezoska, Melissa L. Kelley, and Anja van Brabant Smith Dharmacon, part of GE Healthcare, 2650 Crescent Drive, Lafayette, CO 80026, USA

Chemical synthesis has been easily applied for rapidly generating either crRNA and tracrRNA or synthetic sgRNA for direct delivery into cells for gene editing applications such as DNA-free options and high throughput arrayed screening.

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The Chemical Synthesis of Long and Highly Modified RNA using 2'-ACE Chemistry
Xiaoqin Cheng, Kristina Larson, Letitia Kwok, David Mierzejewski, Shawn Begay, Randy Rauen, Kelly Grimsley, Kaizhang He, Michael Delaney, David Kitchen, Amanda Haas, Melissa Kelley, Anja van Brabant Smith

Dharmacon has previously developed a novel RNA synthesis chemistry making RNA synthesis as reliable, accessible and of comparable quality as routinely observed in DNA synthesis.

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Blood Transcriptomic Diagnosis of Active TB with Five Genes
JK Roe1*, N Thomas1, E Gil1, K Best1, E Tsaliki1, S Morris-Jones2, S Stafford1, N Simpson1, KD Witt3, B Chain1, R Miller4, A Martineau3, M Noursadeghi1

This study aimed to identify the minimal set of genes that can accurately diagnose active TB, both pulmonary and extrapulmonary, and evaluate their specificity.

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Experimental design considerations for efficient and specific gene knockin using a CRISPR-Cas9 for HDR with synthetic crRNA and tracrRNA
Hidevaldo B. Machado, John A. Schiel, Maren Mayer-Gross, Eldon T. Chou, Melissa L. Kelley, Anja van Brabant Smith. Dharmacon, part of GE Healthcare, 2650 Crescent Drive, Lafayette, CO 80026, USA

Precise genome engineering with CRISPR-Cas9 and single-stranded DNA oligo or double-stranded DNA plasmid donors via homology-directed repair (HDR).

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Super-resolution single molecule localization microscopy of the exocytotic machinery underlying insulin secretion.
Deirdre M. Kavanagh, Alison Dun, Rory R. Duncan, and Colin Rickman.

Single molecule imaging of the tSNARE proteins involved in insulin secretion.

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Resistance-Associated Variants in HCV Genotype 1 Populations
Elian Rakhmanaliev1, Pramila Ariyaratne1, Zhang Rui1, Kok Siong Poon2, Cui Wen Chua2, Mui Joo Khoo2, Evelyn S. Koay2, Ekawat Passomsub3, Wasun Chantratita3, Gerd Michel1

In this study we specifically investigated RAVs emerging in HCV genotype 1.

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Integrated Next-Generation Sequencing and qPCR Workflow in Clinical Diagnostics
Elian Rakhmanaliev, Tatiana Ivanova, Gerd Michel

Combined automated qPCR and NGS Sentosa workflow is a reliable and efficient in vitro diagnostics (IVD) tool for the detection and/or quantitation of a wide range of bacterial and viral pathogens as well as gene mutations.

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A Synthetic CRISPR-Cas9 System for Homology-directed Repair
John A. Schiel, Maren M. Gross, Emily M. Anderson*, Eldon T. Chou, Anja van Brabant Smith Dharmacon, part of GE Healthcare, 2650 Crescent Drive, Lafayette, CO 80026, USA

Synthetic, dual-RNA-encoded Cas9 is used for precise homology-directed repair (HDR) gene engineering. Both short and long (GFP) inserts are covered.

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Scientific News
New Mechanism to Control Human Viral Infections Discovered
Researchers discover long sought after mechanism in human cells that could help treat diseases caused by viruses.
Study Reveals New Role for Hippo Pathway in Suppressing Cancer Immunity
Hippo pathway signaling regulates organ size by moderating cell growth, apoptosis and stem cell renewal, but dysregulation contributes to cancer development.
RNAi Activated in Response to Influenza
Discovery could lead to better ways of combating serious infections, including Ebola and Zika.
Gene Therapy Maintains Clotting Factor for Hemophilia Patients
Following a single gene therapy dose, the highest levels of an essential blood clotting factor IX were observed in hemophilia B patients.
Transporting Microscopic Cargo Between Human Cells
Scientists have developed a virus-inspired delivery system for material transport between cells.
Improving Drug Production with Computer Model
A model has been developed that can be used to improve and accelerate the production of biotherapeutics, cancer drugs, and vaccines.
Turning Off Asthma Attacks
Researchers discover a critical cellular “off” switch for the inflammatory immune response that causes asthma attacks.
New Strategy May Drop Cancer’s Guard
Scientists eye ways to deconstruct tumors’ protective wall with current diabetes drug.
Scientists Identify Unique Genomic Features in Testicular Cancer
The findings may shed light on factors in other cancers that influence their sensitivity to chemotherapy.
Smart Patch Releases Blood Thinners When Needed
Researchers have developed a smart patch that activelly monitors a patient's blood and releases blood thinning drugs when necessary.
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