|Repurposing Drugs for the Treatment of Multi-Drug Resistant Breast Cance|
David Monaghan, Rachel Griffin, Amie Regan, Enda O’Connell, Howard Fearnhead
In this study, the Johns Hopkins Clinical Compound Library, containing approximately 1,500 FDA and foreign-approved clinical compounds, was used to screen a multi-drug resistant, triple negative breast cancer cell line for drug sensitivity.
|Intronic polymorphisms in Daucus carota AOX2b generate putative genotype specific miRNA|
Hélia G. Cardoso, Maria Doroteia Campos, Birgit Arnholdt-Schmitt
A study in the carrot alternative oxidase gene DcAOX2b from several individual plant genotypes of D. carota cv. Rotin revealed the frequent occurrence of intron length polymorphisms (ILPs). Here we will present an in silico analysis performed in order to identify putative miRNA sites at three different sizes of intron 1. The overall research approach aims to develop functional marker candidates for carrot plant breeding.
|A high-throughput colony formation assay for profiling novel compounds and RNAi reagents using the Acumen® eX3|
Andrew Goulter and Jason Mundin
Cell colony formation assays measure a cell's ability to grow unattached to a surface and have applications in a range of areas including hematopoietic stem cell research, cell transformation studies and the prediction of responses of tumors to chemotherapeutic agents. The results of this study demonstrated that Acumen eX3 can be used as a high-throughput platform for investigation of effects of test compounds and RNAi reagents on cell colony formation.
|Altering microRNA miR-15a/16 Levels as Potential Therapy in CLL: Extrapolating from the de novo NZB Mouse Model|
Kasar S, Salerno E, Underbayev C, Vollenweider D, Yuan Y and Raveche E
Expression of miR15a/16-1 was increased using lentiviral delivery (in vitro and in vivo) or by BSAP knockdown to inhibit B-CLL malignancy.
|Demethylation and Re-Expression of Tumor Suppressor Genes: A Novel Approach for Cancer Therapy|
Genevieve Housman, Megan A. Mataga, Amrita Devalapalli, Sarah Heerboth, Leah R. Evans, Sibaji Sarkar
In this study, we demonstrated that a combination therapy using suboptimal doses of HDACi and calpeptin, an inhibitor of calpain, produced synergistic type growth inhibition and reduced cancer cell motility in cancer cells. We hypothesize that the re-expression of tumor suppressor genes by demethylation and other mechanisms sensitize the cells and allows for apoptotic death.
|Targeted gene silencing of the MAPK pathway in acute myeloid leukemia cells using RNAi|
Mohd Hafiz bin Mohd Rothi and Mohamed Saifulaman bin Mohamed Said
This study explores the potential of multiple gene knockdown in acute myeloid leukemia of pivotal genes controlling the MAPK pathway using RNA interference. Results demonstrate that blocking a major signaling pathway is more complicated than just knocking down the expression of a few genes.
|Neurocognitive correlates of miRNA expression in the CNS of HIV positive subjects with a history of methamphetamine abuse|
Erick T Tatro, Stephanie Shumaker, David J Moore, Igor Grant, Cristian L Achim
Methamphetamine (meth) abuse and HIV infection are public health risks that in combination produce a “double epidemic.” MicroRNAs (miRs) were shown to be involved in CNS development, neuronal homeostasis, and brain disease. Past studies linked miR124 and let7-d with cocaine addiction. Our goal was to determine whether miRs were differentially expressed in HIV-infected individuals with a recent history of meth abuse and to identify the neurocognitive correlates.
|mSMRT-qPCR : Robust, Sensitive, Scalable microRNA Quantification|
Existing methods for miRNA quantification rely on sequence-dependent probes or chemically modified primers for optimal specificity and often require RNA isolation that is time-consuming labour-intensive and which increase sample variability We have developed a high performance approach for multiplexed detection of mature miRNAs termed modified stem-loop mediated reverse transcription quantitative PCR (mSMRT-qPCR).
|Transfecting Small Molecules, Peptides|
Van Q., Atze K., Litzenberger D., Ackerstaff J.T., Strübing Y., Yolcu D., Franke S.,Mobbs K.J. and Kazinski M.
The Nucleofector® Technology enables efficient and reproducible transfection of primary cells and cell lines at throughputs of up to 96 samples per run. Nucleofection® now extends its range of application to deliver small molecules substrates and protein substrates such as peptides, proteins and antibodyconjugates.