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Print Nanoparticle Vaccine Carrying Bacterial Polysaccharide And Protein Antigens Induces Enhanced B- And T-Cell (Il-17) Immunity
Anton Beletskii,1 Camille Bernasconi,1 Jinny Conley, Meredith Earl,1 Gabe Fawcett,1 Jeremy Hansen,1 Lara Kelly,1 Marquita Lilly,1 Frank Malinoski,1 Joseph Marchand,1 Nicole Meyer,1 Shyam Rele,1 RiLee Robeson,1 Michele Stone,1 Ben Yerxa,1 Jeff Maisonneuve,2 Mark Alderson 2

Antigenic multivalent PRINT nanoparticle formulations leverage precise control of size, shape and composition, sterile filterability and scalable cGMP roll-to roll manufacturing to offering a low cost and simplified manufacturing advantage over traditional conjugate vaccines.

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Design and Evaluation of High Definition Probe for HPV genotyping Microarray
Sihn Ae Lee, Ah Reum Park, Inyoung Kim, Ji Hyung Lee, and Jongwon Kim

To improve the sensitivity and specificity of the HPV DNA Microarray, we adopted triple oligonucleotide probes for each targets and selected these probes not to have higher similarity of 75% with each others. These triple probes have shown 10 ~ 100 times higher sensitivities with comparable specificities than the conventional HPV DNA microarray of single oligonuclotide probe.

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Digital PCR to Determine the Number of Transcripts from Single Neurons after Patch-clamp Recording
Nóra Faragó1,2, Ágnes K. Kocsis3, Sándor Lovas3, Gábor Molnár3, Márton Rózsa3, Viktor Szemenyei3, Ágnes Zvara2, Gábor Tamás3, László G Puskás1,2

Whole-cell patch-clamp recording enables detecting electrophysiological signals from neurons, and RNA can be harvested into the patch pipette from the cells.We have optimized a dPCR protocol for determining exact transcript numbers in single neurons after patch-clamp recording by using dPCR based on high-density nanocapillary PCR.

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Novel Gpr39 Agonists: Correlation Of Binding Affinity Using Label-Free Back-Scattering Interferometry With Potency In Functional Assays
Daniel Brown (1), Niklas Larsson (2), Ola Fjellström (3), Anders Johansson (3), Sara Lundqvist (2), Johan Brengdahl (2), and Richard J. Isaacs (1)

We describe the application of back-scattering interferometry (BSI) to the characterization of small molecule ligand binding to human GPR39 (a GPCR targeted for type-2 diabetes therapy) overexpressed in crude membrane fractions in free solution, including how BSI-derived affinity and functional assay-derived potency correlate for compounds of varying scaffolds.

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Novel Gpr39 Agonists: Correlation Of Binding Affinity Using Label-Free Back-Scattering Interferometry With Potency In Functional Assays
Daniel Brown (1), Niklas Larsson (2), Ola Fjellström (3), Anders Johansson (3), Sara Lundqvist (2), Johan Brengdahl (2), and Richard J. Isaacs (1)

We describe the application of back-scattering interferometry (BSI) to the characterization of small molecule ligand binding to human GPR39 (a GPCR targeted for type-2 diabetes therapy) overexpressed in crude membrane fractions in free solution, including how BSI-derived affinity and functional assay-derived potency correlate for compounds of varying scaffolds

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INTERACTION BETWEEN WHEY PROTEIN NANOPARTICLES AND FATTY ACIDS
Hassan, Z.M.R*, Awad R. A.*, El-Sayed, M. M. **, Mevat I. Foda**, Otzen, D.*** and Heba H. Salama**

Nanocomplexes can be formed from WPI with good cytotoxic effect to tumer cells using cis-vaccenic and linolenic fatty acids comparable to oleic acid. It was a new interesting observation being that the nanocomplexes formed of WPI with fatty acids has a comparable cytotoxcisty to that of a-LA and ß-lg and can be used in tumor therapy.

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A complete workflow from sample preparation to analysis using SureSelect target enrichment system for Ion Proton semiconductor sequencing
Christian Le Cocq, Kyeong Soo Jeong, Arjun Vadapalli, Joseph Ong, Elin Agne, Filip Karlsson, Ashutosh Ashutosh, Francisco Useche, Jayati Ghosh, Henrik Johansson, Scott Happe, Douglas Roberts, and Holly Hogrefe

Agilent’s SureSelect Target Enrichment for the Ion Proton Platform provides a comprehensive, efficient, robust, and cost-effective means to sequence subsets of the human genome.

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Characterization of the prehaustorial resistance against leaf rust (Puccinia triticina f. sp. tritici) in Einkorn (Triticum monococcum) by massive analysis of cDNA ends (MACE)
Albrecht Serfling1,2, Sven Templer1,3, Dragan Perovic1, Frank Ordon1

Triticum monococcum, a valuable source for horizontal resistance against P. triticina was analyzed microscopically and by transcriptional profiling. MACE showed the increased expression of chitinases, kinases, peroxidases and pathogenesis related genes in the first 8 hai. The high number of differentially expressd tags and the knowledge about SNPs facilitates in silico mapping and the development of polymorphic markers which may accelerate the transfer of this prehaustorial resistance

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Population structure and location of genes for resistance to Mycosphaerella graminicola: Recent advances in Argentina
María Rosa Simón1, Cristina A. Cordo2, Nadia S. Castillo3, Elena Khlestkina 4, Paul C. Struik5 and Andreas Börner6

A review about the status of the knowledge on septoria leaf blotch with emphasis in investigations carried out in Argentina is presented

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Scientific News
Two New and Very Large Classes of RNAs Linked to a Cancer Biomarker Identified
Study shows two new classes of RNAs could play a role in progression of prostate cancer.
Master Gene Regulator Could Be New Target For Schizophrenia Treatment
Researchers at MIT’s Picower Institute for Learning and Memory have identified a master genetic regulator that could account for faulty brain functions that contribute to schizophrenia.
Researchers Find “Decoder Ring” Powers in microRNA
MicroRNA can serve as a "decoder ring" for understanding complex biological processes, a team of New York Univ. chemists has found.
Genetic Markers for Detecting and Treating Ovarian Cancer
Custom bioinformatics algorithm identifies human mRNAs that distinguish ovarian cancer cells from normal cells and provide new therapeutic targets
GTEx: Useful Expression For Cancer Research
GTEx Project has recently published several papers reporting on findings from its two-year pilot phase.
Baylor, TGen Collaborate on Personalized Cancer Treatment Options
The companies will collaborate on precision medicine for cancer patients by offering liquid biopsies, performing gene sequencing, conducting clinical trials, and creating personalized vaccines.
It Runs in the Family
Distantly related viruses share a common machinery for replication.
Inexpensive Technique Developed to Manufacture Nanofibers
Current methods for producing nanofibers are incredibly costly and therefore limit accessibility, this new study opens the door to cheap, mass produced nanofibers.
Normal Skin Accrues High Number of Mutations Associated with Cancer
Researchers used genomic sequencing to gain insights into how somatic mutations build up in normal cells before the onset of cancer, a process that is poorly understood.
New Device May Shed Light on Why Cancer Cells Metastasize
Scientists from the University of Michigan think they are now a step closer to understanding why some cancer cells metastasize.
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