|miR-21 upregulation and miR-128a downregulation in human glioblastomas|
P. Costa1,2, A. Cardoso1, L. Almeida1,3, M.C. Pedroso de Lima1,2, P. Canoll4, J. Bruce5
miRNA deregulation is an important hallmark on cancer development and progression. This work shows that miR-21 is overexpressed is human glioblastoma samples and cell lines, whereas miR-128a is highly downregulated on these tumours. miRNA modulation using antisense oligonucleotides and/or miRNA precursor molecules decreased tumor cell viability. Thus, targeting deregulated miRNAs may constitute a complementary/alternative approach on the treatment of glioblastomas and other miRNA-related maligna
|Receptor Interacting Protein 140 (RIP140) silencing via RNAi interferes with fatty acid metabolism but not with glucose uptake in L6 muscle cells|
Constantinescu, S., Turcotte, L.P
The role of RIP140 in the regulation of fatty acid uptake, oxidation and glucose uptake was investigated in muscle cells using RNAi technique. Cells were incubated ±insulin before measurement of glucose and FA kinetics. We suggest that reducing RIP140 expression in muscle has no impact on the regulation of GU but leads to high rates of FAU and reduced sensitivity to insulin as it pertains to FA metabolism.
|Co-rgulation of tumour suppressor gene programmed cell death 4 (PDCD4) by miR-21 and miR-499 in head and neck cancer|
Xiaoying Zhang 1, 2, Barbara Rose 1, 2, Rosetta Martiniello-Wilks3 and Nham Tran 1, 2, 3
This study has identified differentially expressed miRNAs in tonsil cancers and provides new evidence for co-regulation of the oncogene PDCD4 by miR021 and miR-499
|Protein array-based screening of autoantibody signatures|
Zingaretti C., Arigò M., Colombatto P., Brunetto M., Muratori L., Bonino F., Bianchi F., Pagani M., De Francesco R., Abrignani S., Bombaci M.
The evidence for an association between autoimmune diseases and chronic HCV infection has been clearly established. To this aim, a protein array was employed to analyze serum samples of HCV patients w/wo autoimmune complications, of patients with autoimmune hepatitis but not infected with HCV and of healthy donors as controls. A panel of autoantigens able to discriminate among the three groups of patients was identified for potential use as biomarkers.
|Clinical Evaluation of human embryonic stem cells (hESCs) induced with directed differentiation to gonadotrope cells to cure vasculogenic impotency and to improve coital frequency in males. An open st|
Timothy S. Andersson, David K. Chin, Wendy Hiu Wai Wong
This work demonstrates the clinical efficacy, tolerability and safety of patient-syngenic hESC induced with directed differentiation to gonadotrope cells. Hypothalamus transmits gonadotropin releasing factor to pituitary that sets off LH and FSH to Sertoli cell and Seminiferous tubule resulting Leydig cell to produces testosterone. This potential offers a rationale to evaluate hESCs to cure patients with vasculogenic impotency and to improve coital frequency in males.
|Clinical Evaluation of induced pluripotent stem cells (iPSc) to cure patients with Psoriasis Vulgaris. An Open Study.|
Timothy S. Andersson, David K. Chin, Wendy H. Wong
To evaluate the clinical efficacy of iPSc directed to interfere T-cell activation to cure patients with psoriasis. Twenty subjects were enrolled. Each patient was administered iv with syngenic iPS. Those showing lower PASI score were considered cured. The study states that induced iPSCs are safe and impart significant clinical efficacy to cure patients with psoriasis.
|MitoProd patented technology for RNA manufacturing and its novel circular interfering RNA|
Jérome Lacoste*, Guillaume Plane*, and Jean-Paul Di Rago**
Here is a description of MitoProd patented technology for RNA manufacturing, permitting a recurring production of RNA in industrial quantities. This technology enables the production of custom RNA at a gram scale, 99 % full length and 95% pure. MitoProd has also designed a new class of interfering RNA called ciRNA®, which have improved features such as RNAses resistance and an increased in vivo efficiency compared to siRNAs.
|MicroRNA-23b negatively regulates urokinase and c-met and inhibits migration of human hepatocellular carcinoma cells.|
Salvi A, Sabelli C, Moncini S, Venturin M, Arici B ,Riva P, Portolani N, Giulini SM, Barlati S and De Petro G.
By bioinformatics we predicted that miR-23b could recognize two sites in the 3’ UTR of uPA (urokinase-type plasminogen activator) and four sites in the 3’ UTR of c-met (hepatocyte growth factor receptor). miR-23b transfections in SKHep1C3 caused uPA and c-met decreased and migration and proliferation inhibition of SKHep1C3; anti-miR-23b transfection in human fibroblasts upregulated uPA and c-met. uPA and c-met shared a common microRNA that negatively regulates their expression.
|Profiling formalin-fixed, paraffin-embedded (FFPE) samples on three Agilent microarray platforms|
Grazyna Fedorowicz, Srinka Ghosh, Steve Guerrero, Thomas Wu, and Zora Modrusan
Thousands of formalin-fixed, paraffin-embedded (FFPE) samples from clinical archives are available for retrospective studies. Such samples could provide crucial information for drug target discovery and diagnostics of various diseases. Here we used FFPE samples and their matched fresh-frozen (FF) counterparts to examine their performance on three types of microarrays including whole genome expression, comparative genomic hybridization (CGH) and microRNA.