|Demethylation and Re-Expression of Tumor Suppressor Genes: A Novel Approach for Cancer Therapy|
Genevieve Housman, Megan A. Mataga, Amrita Devalapalli, Sarah Heerboth, Leah R. Evans, Sibaji Sarkar
In this study, we demonstrated that a combination therapy using suboptimal doses of HDACi and calpeptin, an inhibitor of calpain, produced synergistic type growth inhibition and reduced cancer cell motility in cancer cells. We hypothesize that the re-expression of tumor suppressor genes by demethylation and other mechanisms sensitize the cells and allows for apoptotic death.
|Targeted gene silencing of the MAPK pathway in acute myeloid leukemia cells using RNAi|
Mohd Hafiz bin Mohd Rothi and Mohamed Saifulaman bin Mohamed Said
This study explores the potential of multiple gene knockdown in acute myeloid leukemia of pivotal genes controlling the MAPK pathway using RNA interference. Results demonstrate that blocking a major signaling pathway is more complicated than just knocking down the expression of a few genes.
|Neurocognitive correlates of miRNA expression in the CNS of HIV positive subjects with a history of methamphetamine abuse|
Erick T Tatro, Stephanie Shumaker, David J Moore, Igor Grant, Cristian L Achim
Methamphetamine (meth) abuse and HIV infection are public health risks that in combination produce a “double epidemic.” MicroRNAs (miRs) were shown to be involved in CNS development, neuronal homeostasis, and brain disease. Past studies linked miR124 and let7-d with cocaine addiction. Our goal was to determine whether miRs were differentially expressed in HIV-infected individuals with a recent history of meth abuse and to identify the neurocognitive correlates.
|mSMRT-qPCR : Robust, Sensitive, Scalable microRNA Quantification|
Existing methods for miRNA quantification rely on sequence-dependent probes or chemically modified primers for optimal specificity and often require RNA isolation that is time-consuming labour-intensive and which increase sample variability We have developed a high performance approach for multiplexed detection of mature miRNAs termed modified stem-loop mediated reverse transcription quantitative PCR (mSMRT-qPCR).
|Transfecting Small Molecules, Peptides|
Van Q., Atze K., Litzenberger D., Ackerstaff J.T., Strübing Y., Yolcu D., Franke S.,Mobbs K.J. and Kazinski M.
The Nucleofector® Technology enables efficient and reproducible transfection of primary cells and cell lines at throughputs of up to 96 samples per run. Nucleofection® now extends its range of application to deliver small molecules substrates and protein substrates such as peptides, proteins and antibodyconjugates.
|SuperNatural: A Database of Available Natural Compounds|
Melanie Füllbeck, Mathias Dunkel and Robert Preissner
The majority of marketed drugs are natural compounds or derivatives thereof. The compounds availability is often unclear. Therefore we have compiled a database of ~50,000 natural compounds. Starting point for in silico screenings are about 2,500 well-known, classified natural compounds or imported molecules. Possible medical applications can be detected and about three million conformers computed to account for the flexibility during usage of the 3D-superposition algorithm.
|MicroRNA let-7b targets Akt-1: possible implication for skeletal muscle atrophy in diabetic rats|
Sousa, TA*; Mitsuo, K**; Paula-Gomes, S*; Silva, VAO*; Tragante, V*; Zanon, NM*; Wang M**; Kettelhut, IC*; Natarajan R**; De Lucca, FL*
In diabetic animals there is marked muscle atrophy, but the molecular mechanisms underlying muscle wasting are still unclear. Our in silico analysis and the inverse correlation between let-7b and Akt1 expression in soleus of diabetic animals indicated Akt1 as a potential let-7b target. The assays of luciferase and inhibition of endogenous expression of Akt1 with mimic let-7b were used to validate Akt1 as a direct target of let-7b.
|Microsphere Approach to Gene Silencing|
J. M. Cardenas-Maestre, A. Seth, R. M. Sanchez-Martin
The design of a new generation of bio-compatible polymeric particles (by introduction of polycationic chains as terminal groups) that can be employed as transfecting agents.
|Argonaute 1 regulates germ cell division and oocyte determination in Drosophila melanogaster|
Mohd Ghows Mohd Azzam and Ji-Long Liu
We find that Ago1 protein is be enriched in the oocytes and also distributed in the cytoplasm of follicle cells. Using mitotic clonal analysis, we analyze multiple ago1 mutant alleles. Here, we find that the oocyte do not form in many mutant clones and they only have 8 nurse cells. These results indicate that the level of Ago1 affects the number of cystoblast divisions and oocyte determination.