|Evaluation of a Novel Approach for the Measurement of RNA Quality|
Timothy Wilkes; Alison Devonshire; Carole Foy
The potential medical applications of microarrays have generated much interest, within the biomedical community. Numerous potential sources of variation have raised concerns regarding assay consistency and data quality. Previous studies have highlighted RNA integrity as having a major effect on data quality. We describe here a comparison of the performance characteristics of the Agilent (Bioanalyser) and Lab901 (Screen Tape System) platforms, and their capacity to determine sample RNA integrity.
|Fabrication and characterization of a fully integrated microdevice for in-vitro single cell assays|
C. Collini, E. Morganti, R. Cunaccia, L. Odorizzi, C. Ress, and L. Lorenzelli (1), A. De Toni, G. Marinaro (2), M. Borgo (3), M. Maschietto (4)
This work presents the microfabrication and preliminary characterization of a fully integrated microdevice for in-vitro single cell assays. This technological platform combines IDEs and MEA-based modules for cell addressed delivery of bio-functionalized nano/microparticles and single cell electroporation respectively.
|Intended transcriptional gene silencing with siRNA to the human Vascular Endothelial Growth Factor (VEGF) promoter results in VEGF repression through sequence-specific off-targeting|
Joshua Moses, Amber Goodchild and Laurent Rivory
SiRNA designed to target the human VEGF gene promoter revealed a putative candidate for transcriptional gene silencing. However, mutation or deletion of the target site demonstrated that the observed VEGF knockdown was the result of a sequence-specific off-target effect. Bioinformatic and microarray analyses of genes implicated in VEGF transcriptional control followed by knockdown and Western blotting experiments presented one candidate, GRB2, as an unintended target of the VEGF promoter-specifi
|Point-of-care testing for prostate cancer diagnosis |
Y. Uludag; I.E. Tothill
This presentation describes the potential of using a new fully automated QCM biosensor and a commercial SPR biosensor for the detection of PSA, a biomarker for prostate cancer. Initially a new buffer was formulated to eliminate 98% of the non-specific human serum protein binding to the sensors. Obtained results indicate that both instruments can be used for the fast and easy detection of PSA (0.29-150 ng/ml) in serum.
|A Web Interface for Automatic Microarray Analysis|
Enrico Glaab, Jonathan M. Garibaldi, Natalio Krasnogor
DNA-Microarray technology provides a diagnostic tool for studying cancer and genetic diseases, allowing the experimenter to identify disease related genes and predict the tumour type for new cell samples. However, the statistical analysis of microarray data is a time-consuming and error-prone process. To address this problem we have developed a web interface for automatic DNA-microarray analysis, ArrayMining.net, which guides the user through the analysis process and performs automatic parameter
|A modular and stochastic approach to the study of gene circuits using P systems|
Romero-Campero FJ, Blakes J, Cao H, Camara M, Krasnogor N.
presented at Genomes to Systems Manchester, UK March 17-19 2008
|Formal informatics and machine learning for more principled systems and synthetic biology|
Romero-Campero FJ, Blakes J, Camara M, Willams P, Perez-Jimenez MJ, Krasnogor N
presented at European Conference on Synthetic Biology (ESF-UB Conference in Biomedicine), Sant Feliu de Guixols, Spain, 24-29 November 2007.
|A systems analysis of the AHL Quorum Sensing system in Pseudomonas aeruginosa|
Romero-Campero FJ, Blakes J, Camara M, Krasnogor N.
presented at Systems Biology (ESF-UB Conference in Biomedicine), Sant Feliu de Guixols, Spain, 12-17 April 2008
|An Integrated Development Environment for Synthetic Biology Models|
Blakes J, Romero-Campero FJ, Twycross J, Cao H, Krasnogor N
presented at European Conference on Synthetic Biology (ECSB) II: Design, Programming and Optimisation of Biological Systems (ESF-UB Conference in Biomedicine), Sant Feliu de Guixols, Spain, 29 March - 03 April 2009