|Recent Technical Developments in the Standardized Separation and Measurement of Extracellular Vesicles|
Amy Phillips (1), Robert Vogel(2), and Murray F. Broom(1)
We present robust and practical solutions for the standardization of EV isolation and concentration measurement. EV concentration also needs to have a defined size range. With these solutions, significant improvement in the reproducibility and comparability of results between different researchers is possible.
|Artificial Multi-Gene Expression Systems Design Service for Natural Compound Formation and Hetero Protein Complexes|
Bernauer, Hubert, Gregor Zipf and Josef Maier
Drug discovery of natural compounds drug development and drug target analyses as well as bioproduction can benefit from artificial genetic systems and constructions. The direction in which genes are to be developed is written in the genomes. Synthesis oriented genomic analyses of codon bias and tRNA adaption analyses are prerequisites for generating adaptive, highly functional genes.
|Identifying Molecular Signatures of Tumors Using Novel Fluorescence Resonance Energy Transfer Networks|
Vishwa Nellore, Chris Dwyer
We developed FRET sensors that can detect 125 fluorophores simultaneously. From experimental analyses of over 1200 time-resolved fluorescence signatures on 300 prototypical sensors, we show that the optical responses are highly repeatable and minor variations between FRET networks can be discriminated resulting in a total of 10^375 unique responses in theory.
|Liposomes and Nanoparticles as Delivery Vehicles for the Treatment of Lung Diseases|
Raisa Kiseleva1, Jennifer Mulligan2, Carl Atkinson2, Rodney Schlossser2, Alexey Vertegel1
Targeted nanoparticle-based drug delivery to the lungs is an emerging area of interest for both scientists and medical workers because it allows better drug retention in the lungs and can provide prolonged drug release. In our study we investigated the potential of using liposomes and polymeric nanoparticles surface modified with a specific antibody as drug carriers for targeted drug delivery to the lungs.
|Amino-Coated Metallofullerene Nanoparticles for Glioblastoma Mutiforme Tumor Detection|
Tinghui Li , Susan. Murphy, Kanwarpal Bakshi, Steven LaConte, Zhi Sheng, and Harry Dorn
We report the preparation of a new functionalized trimetallic nitride endohedral metallofullerene, with a cage surface consisting of positively charged amino groups, which is expected to bind more efficiently to negatively charged cell phospholipid bi-layer cellular surfaces and will more readily undergo endocytosis. We now report that this Gd-nanoplatform when subsequently conjugated with an IL-13 peptide, (IL-13-Gd3N@C80(OH)x(NH2)y) exhibits enhanced targeting of U-251 GBM cell lines.
|Next Generation Multivalent PRINT Nanoparticle Vaccine Targeting Pneumococcal Disease|
Shyam Rele,1 RiLee Robeson,1 Anton Beletskii,1 Jeremy Hansen,1 Meredith Earl,1 Gabe Fawcett,1* Marquita Lilly,1 Joseph Marchand,1 Michele Stone,1 Camille Bernasconi,1 Jinny Conley,1 Michael Hunter,1 Ramya Yadavalli,1 Nicole Meyer,1 Lara Kelly,1 Ben Yerxa,1 Jeff Maisonneuve,2 Mark Alderson,2 Frank Malinoski1
A new generation PRINT nanoparticle Pneumococcal vaccine matrix has been designed to mimic size and structural aspects of bacterial pathogens to efficiently present and deliver polysaccharide and protein antigens to elicit robust immune responses.
|Print Nanoparticle Vaccine Carrying Bacterial Polysaccharide And Protein Antigens Induces Enhanced B- And T-Cell (Il-17) Immunity|
Anton Beletskii,1 Camille Bernasconi,1 Jinny Conley, Meredith Earl,1 Gabe Fawcett,1 Jeremy Hansen,1 Lara Kelly,1 Marquita Lilly,1 Frank Malinoski,1 Joseph Marchand,1 Nicole Meyer,1 Shyam Rele,1 RiLee Robeson,1 Michele Stone,1 Ben Yerxa,1 Jeff Maisonneuve,2 Mark Alderson 2
Antigenic multivalent PRINT nanoparticle formulations leverage precise control of size, shape and composition, sterile filterability and scalable cGMP roll-to roll manufacturing to offering a low cost and simplified manufacturing advantage over traditional conjugate vaccines.
|Design and Evaluation of High Definition Probe for HPV genotyping Microarray|
Sihn Ae Lee, Ah Reum Park, Inyoung Kim, Ji Hyung Lee, and Jongwon Kim
To improve the sensitivity and specificity of the HPV DNA Microarray, we adopted triple oligonucleotide probes for each targets and selected these probes not to have higher similarity of 75% with each others. These triple probes have shown 10 ~ 100 times higher sensitivities with comparable specificities than the conventional HPV DNA microarray of single oligonuclotide probe.
|Digital PCR to Determine the Number of Transcripts from Single Neurons after Patch-clamp Recording|
Nóra Faragó1,2, Ágnes K. Kocsis3, Sándor Lovas3, Gábor Molnár3, Márton Rózsa3, Viktor Szemenyei3, Ágnes Zvara2, Gábor Tamás3, László G Puskás1,2
Whole-cell patch-clamp recording enables detecting electrophysiological signals from neurons, and RNA can be harvested into the patch pipette from the cells.We have optimized a dPCR protocol for determining exact transcript numbers in single neurons after patch-clamp recording by using dPCR based on high-density nanocapillary PCR.