|Efficacy of Gene Therapy in Restoring Vision in Leber's Congenital Amaurosis|
The research reviews current knowledge of the genetic causes of Leber's congenital amaurosis (LCA) and the development of a gene therapy intervention for LCA. The efficacy as well as the limitations of this form of treatment are evaluated in detail and recommendations are made for the future directions of gene therapy for Leber's congenital amaurosis.
|Flow Enhancement and Dynamic of Microfluidic Base Biosensor |
Esmail Abdullah Mohammed Basheer, Hayder A.Abdul Bari
The design of new biosesnor base on microfluidic system is presented. The design is consist of two chips, Microchannels chip and electrodes chip. By varying the size and optimizing the microchannels and electrode, the sensitivity of the biosensor will increase.
|Recent Technical Developments in the Standardized Separation and Measurement of Extracellular Vesicles|
Amy Phillips (1), Robert Vogel(2), and Murray F. Broom(1)
We present robust and practical solutions for the standardization of EV isolation and concentration measurement. EV concentration also needs to have a defined size range. With these solutions, significant improvement in the reproducibility and comparability of results between different researchers is possible.
|Developing a digital histopathology platform to support an international diabetes biobank|
Sucaet Yves,. Smeets Silke, Waelput Wim, In’t Veld P.eter
An online histopathology platform combines tissue samples and online digital histopathology images. We combine detailed online patient data with whole slide images. This allows potential users of the tissue bank material to select the tissue and patient characteristics before entering into a collaborative agreement. The digital histopathology platform also allows different pathology laboratories in Belgium to discuss histopathological slides, and facilitates research collaboration.
|Artificial Multi-Gene Expression Systems Design Service for Natural Compound Formation and Hetero Protein Complexes|
Bernauer, Hubert, Gregor Zipf and Josef Maier
Drug discovery of natural compounds drug development and drug target analyses as well as bioproduction can benefit from artificial genetic systems and constructions. The direction in which genes are to be developed is written in the genomes. Synthesis oriented genomic analyses of codon bias and tRNA adaption analyses are prerequisites for generating adaptive, highly functional genes.
|Identifying Molecular Signatures of Tumors Using Novel Fluorescence Resonance Energy Transfer Networks|
Vishwa Nellore, Chris Dwyer
We developed FRET sensors that can detect 125 fluorophores simultaneously. From experimental analyses of over 1200 time-resolved fluorescence signatures on 300 prototypical sensors, we show that the optical responses are highly repeatable and minor variations between FRET networks can be discriminated resulting in a total of 10^375 unique responses in theory.
|Liposomes and Nanoparticles as Delivery Vehicles for the Treatment of Lung Diseases|
Raisa Kiseleva1, Jennifer Mulligan2, Carl Atkinson2, Rodney Schlossser2, Alexey Vertegel1
Targeted nanoparticle-based drug delivery to the lungs is an emerging area of interest for both scientists and medical workers because it allows better drug retention in the lungs and can provide prolonged drug release. In our study we investigated the potential of using liposomes and polymeric nanoparticles surface modified with a specific antibody as drug carriers for targeted drug delivery to the lungs.
|Amino-Coated Metallofullerene Nanoparticles for Glioblastoma Mutiforme Tumor Detection|
Tinghui Li , Susan. Murphy, Kanwarpal Bakshi, Steven LaConte, Zhi Sheng, and Harry Dorn
We report the preparation of a new functionalized trimetallic nitride endohedral metallofullerene, with a cage surface consisting of positively charged amino groups, which is expected to bind more efficiently to negatively charged cell phospholipid bi-layer cellular surfaces and will more readily undergo endocytosis. We now report that this Gd-nanoplatform when subsequently conjugated with an IL-13 peptide, (IL-13-Gd3N@C80(OH)x(NH2)y) exhibits enhanced targeting of U-251 GBM cell lines.
|Next Generation Multivalent PRINT Nanoparticle Vaccine Targeting Pneumococcal Disease|
Shyam Rele,1 RiLee Robeson,1 Anton Beletskii,1 Jeremy Hansen,1 Meredith Earl,1 Gabe Fawcett,1* Marquita Lilly,1 Joseph Marchand,1 Michele Stone,1 Camille Bernasconi,1 Jinny Conley,1 Michael Hunter,1 Ramya Yadavalli,1 Nicole Meyer,1 Lara Kelly,1 Ben Yerxa,1 Jeff Maisonneuve,2 Mark Alderson,2 Frank Malinoski1
A new generation PRINT nanoparticle Pneumococcal vaccine matrix has been designed to mimic size and structural aspects of bacterial pathogens to efficiently present and deliver polysaccharide and protein antigens to elicit robust immune responses.