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Resorufin - a lead for a new protein kinase CK2 inhibitor
Iben Skjøth Sandholt1, Birgitte Brinkmann Olsen1, Barbara Guerra1, Olaf-Georg Issinger1, Brigitte Boldyreff2

Screening of a natural compound library led to the identification of resorufin, as a highly selective and potent inhibitor for protein kinase CK2. Out of 52 kinases tested only CK2 was inhibited. The IC50 values determined for the CK2 holoenzymes were 1.5 µM and for the free catalytic subunits ca. 4 µM. In different cancer cell lines treatment with resorufin led to cell death and endogenous CK2 was inhibited.

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A Novel Multiplexed Digital Gene Expression Technology
Gary K. Geiss1,#, Roger Bumgarner2, Brian Birditt1, Timothy Dahl1, Naeem Dowidar1, Dwayne L. Dunaway1, H. Perry Fell1, Sean Ferree1, Renee D. George1, Tammy Grogan1, Jeffrey J. James1, Malini Maysuria1, Jeffrey D. Mitton1, Paola Oliveri4, Jennifer L. Osborn3, Tao Peng2, Amber L. Ratcliffe1, Philippa J. Webster1, Eric H. Davidson4, and Leroy Hood5

We describe a novel platform, the nCounter Analysis System, for sensitive, highly multiplexed, digital gene expression analysis based on NanoString’s novel molecular barcoding technology. Detection of individual mRNA molecules using an assigned sequence of six different fluorescent spots per probe are detected, and then the number of times that code sequence appears in a sample are counted.

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Oligonucleotides with LNA and targeting of biologically important RNAs
Peter Guterstam and Ülo Langel

Activity of RNA-targeting antisense oligonucleotides increases when introducing LNA monomers, implying that an 18 nucleotides long LNA/2OMe mixmer splice-switching oligonucleotide can be shortened to 12mer and have similar activity and specificity as an 18mer 2OMe oligonucleoyide. Positioning of LNA monomers has to be carefully considered when utilizing the potent LNA monomers in RNA-targeting antisense oligonucleotides.

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A comparison of siRNA activity predictors using advanced regression techniques
Simone Sciabola, Qing Cao, Theresa Johnson, Lingling Shen, Robert Stanton, Xiaoyu Jiang, Simon Xi, Jason Hughes, Daniel Caffrey, Shobha Potluri, Steven Haney, Johnathan Cyr, Jeremy Little

Although considerable progress has been made recently in understanding how gene silencing is mediated by the RNAi pathway, the design of effective sequences is still a challenging task. To tackle the efficacy challenge and further improve accuracy for prediction of siRNA potency, we performed a comparison of advanced regression techniques and new in-house generated descriptors in the generation of siRNA efficiency models.

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miRNAs in Treating Cardiomyopathy
Veenu Aishwarya

The study aims to design antigomirs against miRNAs involved in Cardiomyopathy. Potential miRNAs involved in the down regulation of certain important genes during this disorder have been identified. All reported miRNAs were scanned using an algorithm against these genes. At three step protocol was followed to take care of false positives and false negatives. Further, HL-1 cells (cardiomyocytes) are been transfected by anti-miRNAs for confirmation.

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qPCRas a primary screen in drug discovery
GauravJaggi, Frank Boeckler, Andreas Joerger and Alan Fersht

We report the use of qPCR technique to follow the thermal unfolding of proteins by the binding of the dye SYPRO Orange, and exploit its potential as a robust and high-throughput primary screen for small molecule drug discovery.

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Targeting Inflammatory Cytokines Using Adenoviruses: gene delivery of biological therapies in ovarian cancer
Michael A. Salako, Hagen Kulbe, Iain A. McNeish, Frances R. Balkwill

Constitutive TNF-alpha expression is characteristic of the malignant ovarian surface epithelium. Adenoviral mutants hold great promise as gene therapy vectors but their efficacy is hindered by an inflammatory cascade orchestrated by TNF-alpha. We found that delivering TNF-alpha shRNA to ovarian cancer cells using oncolytic adenoviruses could reduce the inflammatory cascade generated by adenoviruses and also had direct anti-tumour activity on the cancer cells.

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RNA interference in Manduca sexta induced by double-stranded RNA feeding
Isabel Gomez, Alejandra Bravo and Mario Soberon

We used a double-stranded RNA feeding approach to silence cadherin-like gene: a protein reported as receptor in several lepidopteran insects for the Cry toxins synthesized by Bacillus thuringiensis. We analized the protein expression from dsRNA-fed and untreated laravae on western blott. M. sexta larvae that ingested dsRNA directed against cadherin exhibited a dramatic suppression of the protein and are resistant to the effect of the Cry1Ab toxin.

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Heterochromatin structure is induced by siRNA targeting HIV-1 promoter region
K Suzuki1, H Lim1,3, T Ishida2, T Watanabe2, D Cooper1,3, A Kelleher1,3

We transfected siRNA targeting HIV-1 promoter region into a cell line productively infected with HIV-1. ChIP analysis revealed that the RNA duplex induced transcriptional gene silencing and enrichment of Ago1, H3K9me2, and HDAC1 in HIV promoter region to form heterochromatin structure. The data indicates RNA duplex induces the latent phase of HIV infection, since the chromosome formation is very similar to the state of HIV latently infected cell lines

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Showing Results 81 - 90 of 155
Scientific News
Novel Cancer Vaccine Approach for Brain Tumors
Researchers unravel the mechanisms behind a novel cancer vaccine for brain tumors, paving the way for further development.
New Test Developed at Dana-Farber/Brigham and Women's Cancer Center
Rapid Heme Panel - A high-tech genetic test that speeds treatment decisions for blood cancer patients.
Researchers Silence Leading Cancer-Causing Gene
A novel siRNA-based molecule successfully targets KRAS, a well-studied but hard to halt protein important for cancer development and metastasis.
Tumor Suppressor Also Inhibits Key Property Of Stem Cells
The retinoblastoma protein inhibits cancer by controlling cell division. Now, researchers have shown that it also binds to and inhibits genes necessary for pluripotency.
Almac Announces Launch of CLIA Validated Next Generation Sequencing Assay
P53 considered important biomarker for cancer drug discovery.
Next-Gen Melanoma Drug Excels in Lab Tests
Anti-cancer activity was reported in 10 out of 11 patient tumor samples grown in mice and treated with the experimental drug TAK-733.
Study Identifying Cell of Origin for Large, Disfiguring Nerve Tumors
Lays groundwork for development of new therapies.
Light Shed On Genetic Architecture Of Kidney Cancer
Research reveals link between renal cell carcinoma and exposure to aristolochic acid.
Salk Scientists Discover a Key to Mending Broken Hearts
Researchers regenerate and heal mouse hearts by using the molecular machinery the animals had all along.
First Steps in Formation of Pancreatic Cancer Identified
Researchers at Mayo Clinic’s campus in Jacksonville say they have identified first steps in the origin of pancreatic cancer and that their findings suggest preventive strategies to explore.
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