Corporate Banner
Satellite Banner
RNAi
Scientific Community
 
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
NIH Researchers Identify Striking Genomic Signature for Cancer
Institute has identified striking signature shared by five types of cancer.
CRI Develops Innovative Approach for Identifying Lung Cancer
Institute has developed innovative approach for identifying processes that fuel tumor growth in lung cancer patients.
Counting Cancer-busting Oxygen Molecules
Researchers from the Centre for Nanoscale BioPhotonics (CNBP), an Australian Research Centre of Excellence, have shown that nanoparticles used in combination with X-rays, are a viable method for killing cancer cells deep within the living body.
Crowdfunding the Fight Against Cancer
From budding social causes to groundbreaking businesses to the next big band, crowdfunding has helped connect countless worthy projects with like-minded people willing to support their efforts, even in small ways. But could crowdfunding help fight cancer?
Cancer Cells Kill Off Healthy Neighbours
Cancer cells create space to grow by killing off surrounding healthy cells, according to UK researchers working with fruit flies.
Cancer Drug Target Visualized at Atomic Resolution
New study using cryo-electron microscopy shows how potential drugs could inhibit cancer.
Genetic Mechanism Behind Cancer-Causing Mutations
Researchers at Indiana University has identified a genetic mechanism that is likely to drive mutations that can lead to cancer.
Future of Medicine Could be Found in a Tiny Crystal Ball
A Drexel University materials scientist has discovered a way to grow a crystal ball in a lab. Not the kind that soothsayers use to predict the future, but a microscopic version that could be used to encapsulate medication in a way that would allow it to deliver its curative payload more effectively inside the body.
"Gene Fusion" Drives Childhood Brain Cancers
Study co-led by Penn scientists highlights potential targets for future cancer therapies.
Enzyme Links Age-Related Inflammation, Cancer
Researchers have shown that an enzyme key to regulating gene expression -- and also an oncogene when mutated -- is critical for the expression of numerous inflammatory compounds that have been implicated in age-related increases in cancer and tissue degeneration.
Scroll Up
Scroll Down

Automating Aqueous Compound Solubility Using the 96-Well Multiscreen® Solubility Filter Plate on a Hamilton Microlab® Star Workstation
Bookmark and Share

Hamilton

Determining aqueous compound solubility has become an essential tool in the early stages of the drug discovery process. Solubility of a compound is important in the Biopharmaceutics Classification System (BCS). BCS classifies compounds based on their solubility and permeability. Low solubility can lead to unreliable results during in-vitro testing. Also, insoluble precipitates have been shown to cause false positives in bioassays, wasting valuable time and resources. Such issues will typically add significant cost to drug research projects. In addition to these factors, the standard shake-flask method used to evaluate drug solubility is inherently low throughput and labor intensive.

Here we describe an automated method for determining drug solubility using Millipore’s MultiScreen Solubility filter plate on the Hamilton MICROLAB STAR workstation. The procedure requires minimal manual intervention by utilizing the iSWAP for all plate movements on the deck, but vacuuming occurs offline. The vacuuming occurs off-line because the Hamilton’s Automated Vacuum System does not have a support grid needed for the MultiScreen Solubility plate. Eight compounds were tested in twelve replicates and the results were compared to those achieved through the manual method to demonstrate the viability of the automated protocol. It takes about 1 hour and 48 minutes to process one plate of 96 samples (this includes 90 minute shaking on the deck with a VARIOMAG® shaker). This translates to about six or more plates being able to be processed in a standard 8-hour day.

Further Information


SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,900+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,200+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!