Corporate Banner
Satellite Banner
Scientific Community
Become a Member | Sign in
Home>Videos>This Video


The Epigenetic Regulation of microRNA Genes and Their Role in Cell Invasion and Motility in Human Melanomas
Sandford Burnham Medical Research Institute

MicroRNAs have joined the ranks of important regulatory molecules known to control human cell fate in normal and diseased states. This is also true of cancer cells, including melanoma cells, which are known to undergo progressive epigenetic changes during disease manifestation.   We have identified a group of epigenetically regulated miRNA genes in melanoma cells, and employing direct DNA bisulphite and immunoprecipitated methylated DNA (methyl-DIP) deep-sequencing we have confirmed that the upstream CpG island sequences of several such miRNA genes are hypermethylated in cell lines derived from different stages of melanoma but not in melanocytes and keratinocytes.  Abnormalities in CpG island methylation were also seen in distinct melanoma patient samples classified as primary in situ, regional metastatic, distant metastatic, and nodal metastatic.  The cell line WM1552C derived from a stage 3 melanoma was engineered to artificially express one such epigenetically modified miRNA, miR-34b. This caused a reduction in cell invasion and proliferation rates, suggesting that reduced expression of miR-34b is related to invasiveness and high cell division rate of WM1552C.  RNA samples isolated from WM1552C with or without a miR-34b expression construct were subjected to deep sequencing to identify gene networks around miR-34b. Network modules potentially regulated by miR-34b are related to cytoskeletal remodeling, cell invasion and cell mortality functions, suggesting a mechanism for the role of miR-34b in regulating normal cell motility and cytokinesis.

Request more information
Company product page

Scientific News
Revolutionary Technologies Developed to Improve Outcomes for Lung Cancer Patients
Breath test to detect lung cancer brings oxygen directly to the wound.
New Class of RNA Tumor Suppressors Identified
Two short, “housekeeping” RNA molecules block cancer growth by binding to an important cancer-associated protein called KRAS. More than a quarter of all human cancers are missing these RNAs.
Mathematical Model Forecasts the Path of Breast Cancer
Chances of survival depend on which organs breast cancer tumors colonize first.
Exploring the Causes of Cancer
Queen's research to understand the regulation of a cell surface protein involved in cancer.
Nanocarriers May Carry New Hope for Brain Cancer Therapy
Berkeley lab researchers develop nanoparticles that can carry therapeutics across the brain blood barrier.
RNA-Based Drugs Give More Control Over Gene Editing
CRISPR/Cas9 gene editing technique can be transiently activated and inactivated using RNA-based drugs, giving researchers more precise control in correcting and inactivating genes.
University of Glasgow Researchers Make An Impact in 60 Seconds
Early-career researchers were invited to submit an engaging, dynamic and compelling 60 second video illuminating an aspect of their research.
Metabolic Profiles Distinguish Early Stage Ovarian Cancer with Unprecedented Accuracy
Studying blood serum compounds of different molecular weights has led scientists to a set of biomarkers that may enable development of a highly accurate screening test for early-stage ovarian cancer.
Dead Bacteria to Kill Colorectal Cancer
Scientists from Nanyang Technological University (NTU Singapore) have successfully used dead bacteria to kill colorectal cancer cells.
CRISPR-Cas9 Gene Editing: Check Three Times, Cut Once
Two new studies from UC Berkeley should give scientists who use CRISPR-Cas9 for genome engineering greater confidence that they won’t inadvertently edit the wrong DNA.

SELECTBIO Market Reports
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,800+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,000+ scientific videos