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Resolving Nitrogen-15 and Proton Chemical Shifts for the Mobile Segments of Elastin with Two-Dimensional NMR Spectroscopy

Published: Tuesday, April 10, 2012
Last Updated: Tuesday, April 10, 2012
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In this study, one- and two-dimensional NMR experiments are applied to uniformly- 15N-enriched synthetic elastin, a recombinant human tropoelastin that has been crosslinked to form an elastic hydrogel.

Hydrated elastin is characterized by large segments that undergo “liquidlike” motions that limit the efficiency of cross-polarization. The refocused INEPT experiment is used to target these extensive, mobile regions of this protein. Numerous peaks are detected in the backbone amide region of the protein, and their chemical shifts indicate the completely unstructured, “random coil” model for elastin is unlikely. Instead, more evidence is gathered that supports a characteristic ensemble of conformations in this rubberlike protein.

The full article is published online in The Journal of Biological Chemistry and is free to access.


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