Corporate Banner
Satellite Banner
Scientific Community
Become a Member | Sign in
Home>News>This Article

New White Paper Explores How to Optimize Enzymatic Catalysis in Labs

Published: Monday, October 15, 2012
Last Updated: Monday, October 15, 2012
Bookmark and Share
Less waste, lower costs.

METTLER TOLEDO has announced the publication of a new white paper in its Recent Advances in Organic Chemistry Series entitled “The Next Steps in Enzymatic Catalysis.”

The paper reviews three examples of enzymatic catalysis in military, pharmaceutical and academic labs where ReactIR™ was used to monitor reaction progress.

Robust, real-time in situ monitoring with ReactIR™ allowed researchers to achieve desired results while simultaneously reducing reaction costs and enhancing safety for cleaner, greener chemistry.

Using mid-IR spectroscopy, ReactIR™ creates a “molecular video” of ongoing reactions via a robust probe inserted directly into the reaction vessel.

The elimination of offline sampling provides more reliable data, and fewer interactions with volatile or toxic reaction components.

Concentration changes of all key reactive and transient species can be monitored, allowing for easy mechanism/pathway determination and kinetic parameter definition-critical when reviewing the effectiveness of enzymes as catalysts.

The paper’s authors highlight each context in which ReactIR™ helped answer these and other key reaction questions safely.

In the first example, “Application of Chemoselective Pancreatin Powder-Catalyzed Deacetylation Reaction,” Lek Pharmaceutical researchers used ReactIR™ to study and optimize reaction conditions/catalyst load.

Their experimentation proved that an enzymatic catalysis employed under mild conditions could be high-yielding and economically favorable.

Example two, “Monitoring of Bayer-Villiger Biotransformations of Ketones to Lactones,” used ReactIR™ to monitor reaction progress and kinetics in proof-of-concept work.

ReactIR™ proved a useful tool for the rapid quantification of sensitive enzyme-catalyzed Bayer-Villiger biotransformations using the simplest calibration mode. The complex culture medium in the whole-cell catalysis process did not interfere with results.

In the third and final review, “Enzymatic Hydrolysis of Organophosphates and Organophosphonates,” the inline approach of ReactIR™ allowed quantitative monitoring of reaction progress.

Researchers were able to use small chemical quantities, avoid cross-contamination, and significantly reduce risk of toxic exposure-all measurable gains over other previously-tried methods such as titrimetric pH-stat.

In all examples, ReactIR™ proved non-invasive and non-destructive to chemical reaction components. This allowed researchers to more easily describe each biotransformation and ultimately improve yield.

Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,800+ scientific posters on ePosters
  • More than 4,000+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Mettler-Toledo and Konica Minolta Join Forces
Strategic partnership in the field of multi-parameter analysis for the Flavours & Fragrances industry.
Friday, May 18, 2012
Scientific News
Closing the Loop on an HIV Escape Mechanism
Research team finds that protein motions regulate virus infectivity.
New Analysis Technique for Chiral Activity in Molecules
Professor Hyunwoo Kim of the Chemistry Department and his research team have developed a technique that can easily analyze the optical activity of charged compounds by using nuclear magnetic resonance (NMR) spectroscopy.
Miniaturizable Magnetic Resonance
Microscopic gem the key to new development in magnetic lab-on-a-chip technology.
“Golden Window” in Deep Brain Imaging Opened
The neuroscience community is saluting the creation of a “Golden Window” for deep brain imaging by researchers at The City College of New York led by biomedical engineer Lingyan Shi.
How Viruses Commandeer Human Proteins
Researchers have produced the first image of an important human protein as it binds with ribonucleic acid (RNA), a discovery that could offer clues to how some viruses, including HIV, control expression of their genetic material.
Human Dark Proteome Initiative Launched
Group to focus on advancing research on intrinsically disordered proteins to better understand catastrophic diseases.
Clearest Ever Images of Enzyme that Plays Key Roles in Aging, Cancer
UCLA-led research on telomerase could lead to new strategies for treating disease
Analyzing Protein Structures in Their Native Environment
Enhanced-sensitivity NMR could reveal new clues to how proteins fold.
Proteins with ALS, Cancer Role Do Not Assume a Regular Shape
Our cells contain proteins, essential to functions like protein creation and DNA repair but also involved in forms of ALS and cancer, that never take a characteristic shape, a new study shows.
Studying Bowel Disease With Raman Spectroscopy
inVia confocal Raman microscope used in the study of various childhood diseases.
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,800+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,000+ scientific videos