Corporate Banner
Satellite Banner
Spectroscopy
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Role of Water in Chromosome Spreading and Swelling Induced by Acetic Acid Treatment

Published: Thursday, June 12, 2014
Last Updated: Thursday, June 12, 2014
Bookmark and Share
Researchers analysed intact lymphocytes before and at each stage of the chromosome preparation protocol by FTIR spectroscopy.

Abstract
The so called chromosome preparation is a procedure consisting of three strictly connected stages that enables to obtain chromosomes of quality suitable for cytogenetic analysis. Interestingly, experimental evidence strongly suggested that chromosome spreading and swelling (key processes that allow their counting and detailed structural analysis) are induced in the last fixative-evaporation stage by the interaction, mediated by acetic acid, between water from the environmental humidity, and the cytoplasmic matrix and the chromatin. However, since a considerable variation in the quality of chromosome preparations is observed, strongly depending on the environmental conditions in which the procedure takes place, a better comprehension of the mechanisms underlying chromosome preparation is required. To this aim, here we analysed intact lymphocytes before and at each stage of the chromosome preparation protocol by Fourier transform infrared (FTIR) spectroscopy, a technique widely used for the study not only of isolated biomolecules, but also of complex biological systems, such as whole cells. Interestingly, we found that the chromosome preparation protocol induces significant structural changes of cell proteins and DNA, in particular due to the interaction with acetic acid. Moreover, noteworthy, through the monitoring of changes in the water combination band between 2300 and 1800 cm-1, we provided evidence at molecular level of the crucial role of the bound water to the cytoplasmic matrix and to the chromatin in determining the chromosome spreading and swelling. Our FTIR results, therefore, underline the need to perform the last fixative-evaporation stage in standardized and optimized temperature and relative humidity conditions, thus providing chromosomes of high quality for the cytogenetic analysis that would lead in this way to more reliable results.

The article, Role of Water in Chromosome Spreading and Swelling Induced by Acetic Acid Treatment: A Ftir Spectroscopy Study, is published online in the European Journal of Histochemistry and is free to access. 


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,300+ scientific posters on ePosters
  • More than 4,800+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Diagnosis of Bladder Cancer and Prediction of Survival by Urinary Metabolomics
Bladder cancer seems to be ideal for urinary metabolomics-based diagnosis, as urine can directly contact the cancer lesion in the bladder.
Tuesday, April 22, 2014
A Metabolomic Perspective on Coeliac Disease
Metabolomics has proved to be a useful complement for the characterization of several physiological and pathological conditions and offers promises as a clinical tool.
Monday, April 07, 2014
Evaluation of Thermally Degraded Vegetable Oils using FTIR and GCMS
Researchers report a synchronised signal processing algorithm that caters both FTIR and GCMS signal for evaluation of thermally degraded vegetable oils.
Tuesday, March 11, 2014
FTIR Spectroscopy as a Method to Study Lipid Accumulation in Oleaginous Yeasts
The absorption spectra allows monitoring of the progression of fatty acid accumulation and identification of the most represented classes of the produced lipids.
Tuesday, February 11, 2014
Metabolomics as a Diagnostic Tool in Gastroenterology
This review summarizes contributions that metabolomics has made in IBD and IBS and presents potential future directions within the field.
Thursday, December 12, 2013
Rapid Quantification of Methamphetamine: Using ATR-FTIR and Chemometrics
Researchers have developed novel, robust methods using ATR-FTIR and partial least squares as rapid and inexpensive alternatives to the current UPLC-UV method of methamphetamine quantification.
Wednesday, November 13, 2013
Structural Characterization of Intrinsically Disordered Proteins by NMR Spectroscopy
This review article addresses both NMR experiments and strategies that yield data which can be used for the generation of detailed structural models of IDPs as well as in-cell NMR.
Friday, September 20, 2013
Detection of Gastric Cancer with FTIR Spectroscopy and Support Vector Machine Classification
Research indicates that FT-IR spectroscopy with chemometrics method is reliable, practical, and could be easily implemented in gastric cancer diagnosis.
Friday, September 20, 2013
Effect of Trans Fatty Acid Intake on LC-MS and NMR Plasma Profiles
In this study, NMR and LC-MS untargeted metabolomics has been used as an approach to explore the impact of trans fatty acid intake on plasma metabolites.
Tuesday, August 13, 2013
Applications of Metabolomics in Cancer Research
This review focuses on specific areas within cancer and carcinogenesis research on which metabolomics has been brought to bear.
Monday, July 22, 2013
1H NMR Spectroscopic Quantification of Plasma Metabolites in Dialysate during Hemodialysis
Researchers from Tohoku University have examined the feasibility of using TSP as a quantitative standard of dialysate.
Thursday, May 16, 2013
Use of FT-IR Spectra and PCA to the Bulk Characterization of Cell Wall Residues of Fruits and Vegetables Along a Fraction Process
The study has demonstrated that discrimination between the cell wall residues of fruits and vegetables is possible with the use of FT-IR spectra analysed using the PCA method.
Thursday, May 16, 2013
Triple Resonance Three-Dimensional Protein NMR: Before it Became a Black Box
This account provides a personal perspective on three-dimensional triple resonance experiments, providing some historical background.
Monday, April 29, 2013
Raman Microspectroscopy as a Biomarking Tool for In Vitro Diagnosis of Cancer: A Feasibility Study
The potential of Raman and infrared spectroscopy to become complementary to standard histology has recently intensified.
Monday, February 18, 2013
Crowding, Molecular Volume and Plasticity: An Assessment Involving Crystallography, NMR and Simulations
It is suggested that crowding leads to compaction of protein structure and influences the functionally important plasticity of the molecule.
Wednesday, December 05, 2012
Scientific News
Improving Wheat Crops in the Field
Agrii, RAGT and the University of Nottingham are developing better disease management and yield production in wheat crops using ASD FieldSpec Handheld 2 portable spectroradiometers.
Monitoring Changes, Variability in Blood
Raman spectroscopy used as a tool for monitoring biochemical changes and inter-donor variability in stored red blood cell units.
University Uses Magritek Benchtop NMR to Teach Organic Chemistry
Magritek reports on the benefits of their Spinsolve Benchtop NMR spectrometer for teaching practical organic chemistry to undergraduate students at Cleveland State University in Ohio.
Tracking The Aluminum Used To Purify Tap Water
Kobe University researchers demonstrate a new analysis method to measure the concentration of aluminium used to purify tap water.
New Imaging Method Reveals Nanoscale Details about DNA
Enhancement to super-resolution microscopy shows orientation of individual molecules, providing a new window into DNA’s structure and dynamics.
Scientific Gains May Make Electronic Nose the Next Everyday Device
UT Dallas team breathes new life into possibilities by using CMOS integrated circuits technology.
What Makes a Good Scientist?
It’s the journey, not just the destination that counts as a scientist when conducting research.
Copper is Key in Burning Fat
Berkeley Lab scientist says results could provide new target for obesity research.
Unveiling the Complexity of Mysterious Protein Folding
Imagine trying to reverse engineer a car when all you have is a finished product or a box full of parts — no instructions.
New System for Detecting Explosives
Spectroscopic system with chip-scale lasers cuts detection time from minutes to microseconds.
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,300+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,800+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!