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A Novel Approach Toward Microfluidic Drug Metabolite Synthesis – Electrosynthetic Methodology Simulating Cytochrome (CYP450) Oxidation
Romain Stalder, Gregory P. Roth and Philip Podmore

A novel microfluidic technology and electrochemical synthesis method is demonstrated for the efficient generation of known drug metabolites. These metabolites are typically generated on first pass hepatic oxidation in vivo. The FLUX Module, a new microfluidic electrochemical cell manufactured by Syrris Ltd., has been employed to generate the metabolites of five commercial drugs: Tolbutamide, Chlorpromazine, Diclofenac, Primidone and Albendazole.

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A complete workflow from sample preparation to analysis using SureSelect target enrichment system for Ion Proton semiconductor sequencing
Christian Le Cocq, Kyeong Soo Jeong, Arjun Vadapalli, Joseph Ong, Elin Agne, Filip Karlsson, Ashutosh Ashutosh, Francisco Useche, Jayati Ghosh, Henrik Johansson, Scott Happe, Douglas Roberts, and Holly Hogrefe

Agilent’s SureSelect Target Enrichment for the Ion Proton Platform provides a comprehensive, efficient, robust, and cost-effective means to sequence subsets of the human genome.

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Characterization of the prehaustorial resistance against leaf rust (Puccinia triticina f. sp. tritici) in Einkorn (Triticum monococcum) by massive analysis of cDNA ends (MACE)
Albrecht Serfling1,2, Sven Templer1,3, Dragan Perovic1, Frank Ordon1

Triticum monococcum, a valuable source for horizontal resistance against P. triticina was analyzed microscopically and by transcriptional profiling. MACE showed the increased expression of chitinases, kinases, peroxidases and pathogenesis related genes in the first 8 hai. The high number of differentially expressd tags and the knowledge about SNPs facilitates in silico mapping and the development of polymorphic markers which may accelerate the transfer of this prehaustorial resistance

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Genetic progress in the Romanian triticale breeding program
Ittu Gheorghe*, Saulescu Nicolae, Ittu Mariana, Mustatea Pompiliu and Marinciu Cristina

Since 1984 yield raised up to 43 kg / ha¹ and year¹ or 0.74 % /year¹ this progress being achieved mainly by improving fertility of spikes, plumpness of kernel, the test weight and introduction of short straw RhtB1b (Rht1) and Ddw1 (Hl) genes. In order to improve the yield stability, under predictions of global climatic changes, improvement of genetic diversity to powdery mildew, leaf and yellow rusts, virus and spike diseases and also for pre-harvest sprouting are still demanded.

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Genotyping-by-Sequencing of a set of diverse spring barley (Hordeum vulgare) accessions
Tina Lüders (1), Jens Keilwagen (2), Neele Wendler (3), Axel Himmelbach (3), Rajiv Sharma (3), Benjamin Kilian (3,4), Nils Stein (3), Frank Ordon (1)

The poster presents a Genotyping-by-Sequencing (GBS) approach to saturate a set of diverse spring barley accessions with a high density of SNP markers.

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Genotyping-by-Sequencing of a set of diverse spring barley (Hordeum vulgare) accessions
Tina Lüders (1), Jens Keilwagen (2), Neele Wendler (3), Axel Himmelbach (3), Rajiv Sharma (3), Benjamin Kilian (3,4), Nils Stein (3), Frank Ordon (1)

The poster presents a Genotyping-by-Sequencing (GBS) approach to saturate a set of diverse spring barley accessions with a high density of SNP markers.

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A non-destructive method to assess changes in the biomass of small-grain cereals under field conditions
D. Villegas1, J. Lozano2 and C. Royo1

The poster describes a non-destructive method to assess biomass based on remote sensing techniques. This method is suitable for field phenotyping and it is useful to assess biomass evolution as well as to estimate radiation use efficiency.

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Genetic integrity of rice seeds during ageing and gene bank storage by SSR analysis
I. O. Daniel, K. A. Adeboye, O. W. Adabale, E. S. Aladele and A. Boerner

Rice seeds loose germination capacity with ageing. Loses in genetic integrity was found to occur during artificial ageing at a time when percentage germination declined to 54%.

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Characterization of nitrogen use efficiency in a winter wheat collection
István Monostori, Tamás Árendás, Fruzsina Szira, Gábor Galiba, Borbála Hoffmann, Attila Vágújfalvi

The first-year dataset of the three year experiment has been analysed. To find out, which genotype is recommended for intensive and which one is for extensive cultivation, the performances of the varieties were characterized under low and also under favourable nitrogen supply. The varieties were also ranked based on the increased level of their performance due to the nitrogen supply. Correlations between the measured phenotypic data and the NUE parameters were also calculated.

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Analysis of the genomic structure of a wheat NIL population segregating for resistance to glume blotch with a 90k ILLUMINA SNP chip
Javier Sanchez-Martin1, Simon Krattinger1, Margarita Shatalina1,2, Thomas Wicker1 and Beat Keller1*

Analysis of the genomic structure of a wheat NIL population segregating for resistance to glume blotch with a 90k ILLUMINA SNP chip

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Scientific News
Watching Molecules ‘Dance’ in Real Time
Trapping light at the nanoscale enables real-time monitoring of individual molecules bending and flexing may aid in our understanding of how changes within a cell can lead to diseases such as cancer.
How to Prevent Organic Food Fraud
A new test under development has the potential to authenticate organic tomatoes and other produce.
3-in-1 Spectroscopy System Improves Skin Cancer Detection
The new device may detect cancerous skin lesions early on, leading to better treatment outcomes and ultimately saving lives.
Minuscule Chips for NMR Spectroscopy Promise Portability, Parallelization
Two-by-two-millimeter spectrometer dramatically shrinks footprint for multidimensional analysis of molecules.
NIST Instrument Enables High-speed Chemical Imaging of Tissues
Researchers have demonstrated a dramatically improved technique for analyzing biological cells and tissues based on characteristic molecular vibration "signatures."
New Spectroscopy Technique Provides Unprecedented Look into Photochemical Reactions
Two-dimensional electronic-vibrational spectroscopy can be used to simultaneously monitor electronic and molecular dynamics on a femtosecond time-scale.
X-rays Used to Examine How DNA Protects Itself from UV Light
Scientists have made detailed observations of a “relaxation response” that protects these molecules, and the genetic information they encode, from UV damage.
Using Spectroscopy to Diagnose Skin Cancer
Autofluorescence and diffuse reflectance spectroscopy identify malignant melanoma with 93% accuracy.
Role of Water in Chromosome Spreading and Swelling Induced by Acetic Acid Treatment
Researchers analysed intact lymphocytes before and at each stage of the chromosome preparation protocol by FTIR spectroscopy.
Studying Historic Materials with a Vis-NIR Portable Spectrometer
Analytik have reported how the LabSpec portable spectrometer from PANalytical Boulder is being used in the world of heritage research.
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