|Bovine RNA-seq data analysis of liver and pituitary gland|
Pareek CS12, Smoczynski R12, Dziuba P12, Sikora M12, Golebiewski M2, Blaszczyk P12, Gelfand B3, Yaping F3, Kumar D3.
Two key applications of RNA-seq i.e., i) transcriptome read mapping to a reference genome and ii) SNP detections were investigated to analysis of bovine liver and pituitary gland transcriptome. Here, we have presented ONLY the obtained results of bovine pituitary gland.
|A proteomic analysis of p27kip1-binding proteins reveals a putative role in transcription regulation through RNA polymerase II interaction|
Juan Triviño Paredes1, Atilla Biçer1, Arnauld Besson2, Edurne Gallastegui1, Josep Maria Estanyol3, Maria Jesus Pujol1 and Oriol Bachs1
A proteomic analysis of p27kip1-binding proteins reveals a putative role in transcription regulation through RNA polymerase II interaction
|Characterization of Protein and Protein Aggregates using Temperature-controlled Hollow-Fiber Flow-FFF|
Trevor Havard, Florian Meier, Evelin Moldenhauer, Soheyl Tadjiki, Thorsten Klein
Reproducibilty Improvements in Field Flow Fractionation can be achieved on two frounts.
1. Instrument design and control, the system used in for this poster is does not require flow controllers or switching valves and there for produces the same conditions in every case.
2. Fractionater design, the design of the cartridges used in this poster maintain excellent conditions to maintain constant pressure at the membrane removing unwanted effects of sale relaxation above the membrane s
|Innovative technology that enables RNAi in difficult to transfect cells|
Christina Yamada, Kathryn Robinson, Allison St. Amand, Zaklina Strezoska, Greg Wardle, Anastasia Khvorova, Devin Leake
Investigations at Dharmacon have led to the development of innovative siRNA molecules that can be delivered into difficult-to-transfect cells without additional lipid reagents, virus, or instruments. This technology, Accell siRNA reagents, enables gene knockdown for functional genomic studies in a wide variety of cell types. In some instances, cells can be continuously dosed with Accell siRNAs to enable target gene knockdown for extended durations.
|THe AtSCL26 transcription factor controls cross-talk between GA and N root architecture in Arabidopsis thaliana roots|
Beatriz Lagunas, Anthony D. Carter, Dafyd Jenkins and Miriam Gifford
Phenotypic and molecular evidence supports the hypothesis that developmental program enabling nodule formation arose during evolution from a lateral root ‘blueprint’ pre-existing in all higher plants . We reasoned that analyzing Arabidopsis genes orthologous to regulators of nodulation could shed insight on control of lateral root development. This led us to the discovery that an Arabidopsis GRAS family transcription factor controls lateral root development under specific nitrogen conditions.
|LOHA Comprehensive Assay for Single Nucleotide Polymorphism, Copy Number Variants and Loss of Heterozygosity Using SureSelect Target Enrichment|
Kyeong Soo Jeong, Arjun Vadapalli, Ashutosh Ashutosh, Paula Costa, Brian Peter, Stephanie Fulmer-Smentek, Magnus Isaksson, Jayati Ghosh, Douglas Roberts, Holly Hogrefe
Here we describe a comprehensive assay that enables researchers to identify SNP, INDEL, CNV, and LOH using SureSelect target enrichment. This design can be employed as a standalone entity or in concert with other bait designs for SNP and INDEL detection. We also describe methods for data analysis and visualization.
|An examination of specific cellular organelle-targeting nanotags using combined 3D Raman and SERS imaging |
Katherine Lau, Sarah McAughtrie, Karen Faulds, Duncan Graham
We investigated the specific targeting of endoplasmic reticulum and trans-Golgi network in Chinese hamster ovarian cells using functionalised nanotags. The targeting was examined using the combined 3D SERS and Raman imaging method.
|DETERMINATION OF THE QUALITY OF ACTIVE INGREDIENTS IN PAIN KILLERS USING GC-MS|
Elizabeth N.M Murago1, Nathan Oyaro1, Anthony N. Gachanja, Onditi O. Anam, Felix M. Mawili, Steve Lancaster
From this study, the pain killers sampled were found to have large error bars suggesting that there exist counterfeit drugs in the market. The brands mostly affected for analysis of acetaminophen were panadol, action, P500, P5500, elymol and neladol. The error bars for caffeine analysis were quite low indicating that all tablets either counterfeit or original maintained the same amount of this active ingredient.
|DETERMINATION OF ESSENTIAL AND HEAVY METALS IN KENYAN HONEY BY ATOMIC ABSORPTION AND EMMISSION SPECTROSCOPY|
A. Mbiri, A. Onditi, N. Oyaro
Generally, honey samples recorded very low concentrations of As compared to the other heavy metals determined in this study. The highest concentration of As was recorded in honey sample from Nairobi with a value of 0.03±0.01 ppm. All the values recorded were within the KEBS permitted value of 0.5 ppm in food according to Kenya Bureaue of Standards, 1996.
|Prospecting For Iron and Titanium Using Termitaria|
Kinachu James. G, Kiptoo Jackson. K, Onditi Anam.O.
This study demonstrated elevated levels of the two metals (that is iron and titanium) in termitaria soil compared to the surrounding soils. Termitaria are very important bioturbators and can therefore be used as preliminary step in mineral prospecting since they provide an indication of the potential of the positive anomaly, and enables a judgment on the scale of the metal accumulation
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