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Monday, September 22, 2014
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Design and Evaluation of High Definition Probe for HPV genotyping Microarray
Sihn Ae Lee, Ah Reum Park, Inyoung Kim, Ji Hyung Lee, and Jongwon Kim

To improve the sensitivity and specificity of the HPV DNA Microarray, we adopted triple oligonucleotide probes for each targets and selected these probes not to have higher similarity of 75% with each others. These triple probes have shown 10 ~ 100 times higher sensitivities with comparable specificities than the conventional HPV DNA microarray of single oligonuclotide probe.

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Gut Microbial Metabolites and Hepatic Xenobiotic Metabolism: A High Throughput Screening Approach
Glynn Martin, James Sidaway, Jonathan Swann

This poster highlights the combination of metabonomics and high throughput screening by the identification of gut microbial metabolites and a screening assay designed to determine their cytotoxicity to liver-like cell cultures.

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Digital PCR to Determine the Number of Transcripts from Single Neurons after Patch-clamp Recording
Nóra Faragó1,2, Ágnes K. Kocsis3, Sándor Lovas3, Gábor Molnár3, Márton Rózsa3, Viktor Szemenyei3, Ágnes Zvara2, Gábor Tamás3, László G Puskás1,2

Whole-cell patch-clamp recording enables detecting electrophysiological signals from neurons, and RNA can be harvested into the patch pipette from the cells.We have optimized a dPCR protocol for determining exact transcript numbers in single neurons after patch-clamp recording by using dPCR based on high-density nanocapillary PCR.

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Rapid Quantification of Proteins in Complex Matrices using the DeNovix DS11 Microvolume Spectrophotometer
Mebs A Surve & Dan Schieffer

In this poster, we will introduce the DeNovix DS-11 as the next generation in microvolume spectrophotometry.

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Mixtures Analysis of Complex Mixtures
Michael Bernstein; Carlos Cobas; Santi Domínguez; Manuel Pérez; Agustín Barba

We describe an NMR method to quantify mixture components in wine, edible oils, etc. The method is fully customizable, and amenable to high throughput operation.

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A Complete Wine Analysis Using Multiplets Detection
Dr Michael Bernstein1; Agustín Barba1; Dr Susanne Klein2; Dr Andrea Dreiseitel2; Daniel Heidger2 and Volker Heidger2

NMR mixtures analysis can be used to determine the concentration of key components in wine. Here we show the analysis using SMA from Mestrelab.

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A Novel Approach Toward Microfluidic Drug Metabolite Synthesis – Electrosynthetic Methodology Simulating Cytochrome (CYP450) Oxidation
Romain Stalder, Gregory P. Roth and Philip Podmore

A novel microfluidic technology and electrochemical synthesis method is demonstrated for the efficient generation of known drug metabolites. These metabolites are typically generated on first pass hepatic oxidation in vivo. The FLUX Module, a new microfluidic electrochemical cell manufactured by Syrris Ltd., has been employed to generate the metabolites of five commercial drugs: Tolbutamide, Chlorpromazine, Diclofenac, Primidone and Albendazole.

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A complete workflow from sample preparation to analysis using SureSelect target enrichment system for Ion Proton semiconductor sequencing
Christian Le Cocq, Kyeong Soo Jeong, Arjun Vadapalli, Joseph Ong, Elin Agne, Filip Karlsson, Ashutosh Ashutosh, Francisco Useche, Jayati Ghosh, Henrik Johansson, Scott Happe, Douglas Roberts, and Holly Hogrefe

Agilent’s SureSelect Target Enrichment for the Ion Proton Platform provides a comprehensive, efficient, robust, and cost-effective means to sequence subsets of the human genome.

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Characterization of the prehaustorial resistance against leaf rust (Puccinia triticina f. sp. tritici) in Einkorn (Triticum monococcum) by massive analysis of cDNA ends (MACE)
Albrecht Serfling1,2, Sven Templer1,3, Dragan Perovic1, Frank Ordon1

Triticum monococcum, a valuable source for horizontal resistance against P. triticina was analyzed microscopically and by transcriptional profiling. MACE showed the increased expression of chitinases, kinases, peroxidases and pathogenesis related genes in the first 8 hai. The high number of differentially expressd tags and the knowledge about SNPs facilitates in silico mapping and the development of polymorphic markers which may accelerate the transfer of this prehaustorial resistance

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Genetic progress in the Romanian triticale breeding program
Ittu Gheorghe*, Saulescu Nicolae, Ittu Mariana, Mustatea Pompiliu and Marinciu Cristina

Since 1984 yield raised up to 43 kg / ha¹ and year¹ or 0.74 % /year¹ this progress being achieved mainly by improving fertility of spikes, plumpness of kernel, the test weight and introduction of short straw RhtB1b (Rht1) and Ddw1 (Hl) genes. In order to improve the yield stability, under predictions of global climatic changes, improvement of genetic diversity to powdery mildew, leaf and yellow rusts, virus and spike diseases and also for pre-harvest sprouting are still demanded.

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Showing Results 1 - 10 of 40
Scientific News
A Molecular Physics Experience Through Movement at Stanford
dS, short for danceroom Spectroscopy, is the world's first large-scale, interactive molecular physics experience.
Watching Molecules ‘Dance’ in Real Time
Trapping light at the nanoscale enables real-time monitoring of individual molecules bending and flexing may aid in our understanding of how changes within a cell can lead to diseases such as cancer.
How to Prevent Organic Food Fraud
A new test under development has the potential to authenticate organic tomatoes and other produce.
3-in-1 Spectroscopy System Improves Skin Cancer Detection
The new device may detect cancerous skin lesions early on, leading to better treatment outcomes and ultimately saving lives.
Minuscule Chips for NMR Spectroscopy Promise Portability, Parallelization
Two-by-two-millimeter spectrometer dramatically shrinks footprint for multidimensional analysis of molecules.
NIST Instrument Enables High-speed Chemical Imaging of Tissues
Researchers have demonstrated a dramatically improved technique for analyzing biological cells and tissues based on characteristic molecular vibration "signatures."
New Spectroscopy Technique Provides Unprecedented Look into Photochemical Reactions
Two-dimensional electronic-vibrational spectroscopy can be used to simultaneously monitor electronic and molecular dynamics on a femtosecond time-scale.
X-rays Used to Examine How DNA Protects Itself from UV Light
Scientists have made detailed observations of a “relaxation response” that protects these molecules, and the genetic information they encode, from UV damage.
Using Spectroscopy to Diagnose Skin Cancer
Autofluorescence and diffuse reflectance spectroscopy identify malignant melanoma with 93% accuracy.
Role of Water in Chromosome Spreading and Swelling Induced by Acetic Acid Treatment
Researchers analysed intact lymphocytes before and at each stage of the chromosome preparation protocol by FTIR spectroscopy.
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