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Increasing gene editing efficiencies in eukaryotic cell lines by selection of appropriate CRISPR-Cas9 reagents
Melissa L. Kelley, Žaklina Strezoska, Elena Maksimova, Hidevaldo Machado, Emily M. Anderson, Maren Mayer, Annaleen Vermeulen, Shawn McClelland, Anja van Brabant Smith

Overview of various CRISPR-Cas9 reagents to provide the highest efficiency of gene editing in your experiments.

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Knockdown of p53 by Accell self-delivering siRNA causes inhibition of p53-dependent DNA damage response in IMR-32 neuroblastoma cell line and β-amyloid toxicity in rat cortical neurons
Žaklina Strezoska, Tamara Seredenina1, Devin Leake, Annaleen Vermeulen

Here we describe how application of Accell siRNA enabled the development of a high content screening assay in IMR-32 neuroblastoma cells and a whole culture cell viability assay in primary rat cortical neurons.

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High Performance Comprehensive Two-Dimensional Gas Chromatography Coupled with a High Resolution Multi-Reflecting TOFMS for Confident Non-Target Analyte Identification
Scott J. Pugh, Viatcheslav Artaev, Mark F. Merrick, Jack Cochran

The use of comprehensive two-dimensional gas chromatography to help increase chromatographic resolution is a major step in tackling the problem of confident peak identification in a complex sample matrix. Combining the separation power of two-dimensional gas chromatography, with resolving power greater than 25,000, and sub ppm mass accuracies of a high resolution multi-reflecting TOFMS is the ideal solution to confident compound identification within a complex sample matrix.

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An Efficient Method for the Incorporation of Molecular Probes at Multiple/Specific sites in RNA: Levulinyl Protection for 2'-ACE ® , 5'-Silyl Oligoribonucleotide Synthesis
Xiaoqin Cheng, Shawn Begay, Randy Rauen, Kelly Grimsley, Kaizhang He, Michael Delaney

A unique method that uses a levulinate ester as a protecting group to introduce conjugates or molecular probes to virtually any location in a synthetic RNA molecule is discussed. The Levulinyl protecting group is stable in RNA synthesis conditions and can be removed without affecting the other parts of the synthesized RNA. We show the capabilities of this approach with three high-complexity synthesis examples.

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Bovine RNA-seq data analysis of liver and pituitary gland
Pareek CS12, Smoczynski R12, Dziuba P12, Sikora M12, Golebiewski M2, Blaszczyk P12, Gelfand B3, Yaping F3, Kumar D3.

Two key applications of RNA-seq i.e., i) transcriptome read mapping to a reference genome and ii) SNP detections were investigated to analysis of bovine liver and pituitary gland transcriptome. Here, we have presented ONLY the obtained results of bovine pituitary gland.

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A proteomic analysis of p27kip1-binding proteins reveals a putative role in transcription regulation through RNA polymerase II interaction
Juan Triviño Paredes1, Atilla Biçer1, Arnauld Besson2, Edurne Gallastegui1, Josep Maria Estanyol3, Maria Jesus Pujol1 and Oriol Bachs1

A proteomic analysis of p27kip1-binding proteins reveals a putative role in transcription regulation through RNA polymerase II interaction

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Impact of the transgene MON 810 in expression of coding and non-coding RNAs in maize
Rodrigues, M.1, Chegão, A. 1, Costa, A. 1, Fernandes, C.1, Quedas, F. 2, de Andrade, E.1

We demonstrate that plant improvement by means of transgenesis causse alterations in the expression profiles of endogenous non-target genes.

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Characterization of Protein and Protein Aggregates using Temperature-controlled Hollow-Fiber Flow-FFF
Trevor Havard, Florian Meier, Evelin Moldenhauer, Soheyl Tadjiki, Thorsten Klein

Reproducibilty Improvements in Field Flow Fractionation can be achieved on two fronts. Instrument design and control, the system used in for this poster is does not require flow controllers or switching valves and there for produces the same conditions in every case. Fractionater design, the design of the cartridges used in this poster maintain excellent conditions to maintain constant pressure at the membrane removing unwanted effects of sale relaxation above the membrane s

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Innovative technology that enables RNAi in difficult to transfect cells
Christina Yamada, Kathryn Robinson, Allison St. Amand, Zaklina Strezoska, Greg Wardle, Anastasia Khvorova, Devin Leake

Investigations at Dharmacon have led to the development of innovative siRNA molecules that can be delivered into difficult-to-transfect cells without additional lipid reagents, virus, or instruments. This technology, Accell siRNA reagents, enables gene knockdown for functional genomic studies in a wide variety of cell types. In some instances, cells can be continuously dosed with Accell siRNAs to enable target gene knockdown for extended durations.

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Scientific News
Using Light To Examine The Lungs Of Premature Babies
New technique has potential to replace the use of X-rays to see how much air babies’ lungs contain.
Novel Spectroscopy by Using Aberrations
Flaws inherent to electron microscopy used to create probes for performing novel atomic-level spectroscopy.
Effective Identification of Low-Gliadin Wheat Lines
Researchers have demonstrated the use of NIRS to identify low-gliadin wheat lines.
Prostate Cancer Surgery Improved
Researchers at UT Southwestern Medical Center have determined that light reflectance spectroscopy can differentiate between malignant and benign prostate tissue with 85 percent accuracy, a finding that may lead to real-time tissue analysis during prostate cancer surgery.
Faster UVA Molecular Analysis Technology
There are people in the world – chemical engineers, astronomers, national defense scientists investigating an explosion – who need to know just what something is made of, down to the molecular level.
Properties of Light Can be Controlled by Nanostructures
A study led by the UPV/EHU-University of the Basque Country professor Ángel Rubio has simulated a new device to generate terahertz radiation using carbon nanostructures.
Infrared Spectrometer ‘Engine’ for Developers
Si-Ware Systems has launched volume production of the smallest, lowest-cost infra-red spectrometer “engine” for developers.
Breaking the Chain
Compound prevents multidrug-resistant fungi from pumping out drugs.
Low-Cost, Portable NQR Spectroscopy
A researcher at Case Western Reserve University is developing a low-cost, portable prototype designed to detect tainted medicines and food supplements that otherwise can make their way to consumers. The technology can authenticate good medicines and supplements.
Structure of Brain Plaques in Huntington's
Researchers at the University of Pittsburgh School of Medicine have shown that the core of the protein clumps found in the brains of people with Huntington's disease have a distinctive structure, a finding that could shed light on the molecular mechanisms underlying the neurodegenerative disorder.
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