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Quantitation Limit of Pharmaceuticals by UV Spectrophotometry
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Shimadzu

To verify these requirements, the cleaning itself must be evaluated, and the analytical instruments typically used for cleaning validation are the ultraviolet-visible spectrophotometer, the total organic carbon analyzer, and the high-performance liquid chromatograph. The quantitation limit of an analytical instrument is the limit value at which residual sample can be quantitated. This Application Note outlines the determination of quantitation limit for the Shimadzu UV-1800 ultraviolet-visible spectrophotometer by absorption photometry.

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