Corporate Banner
Satellite Banner
Scientific Community
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
Michigan Researchers Use Raman Spectroscopy
inVia confocal Raman microscope used in the study of various childhood diseases.
Pittcon Announces Sanford Asher as Recipient of the 2016 SACP Award
Asher will accept this prestigious award at Pittcon 2016 in Atlanta, Georgia.
Metamaterial Absorbers for Infrared Inspection Technologies
A metamaterial absorber capable of highly sensitive infrared detection enhances the spectroscopy of tiny molecular details.
Pancreatic Cancer Stem Cells Could be "Suffocated" by Anti-diabetic Drug
A new study shows that pancreatic cancer stem cells (PancSCs) are virtually addicted to oxygen-based metabolism, and could be “suffocated” with a drug already used to treat diabetes.
Marzipan Made From Pure Almonds
Researchers carry out DNA analyses and use advanced protein identification techniques to determine whether marzipan is made from pure almonds or also contains other nuts or beans.
Evidence of Ancient Life Discovered Deep Below the Seafloor
Ancient rocks harbored microbial life deep below the seafloor, reports a team of scientists from the Woods Hole Oceanographic Institution (WHOI), Virginia Tech, and the University of Bremen.
Kwansei Gakuin University in Hyogo, Japan, Uses Raman Microscopy
Raman Microscopy study crystallographic defects in silicon carbide wafers.
Structural Discoveries Could Aid in Better Drug Design
Scientists have uncovered the structural details of how some proteins interact to turn two different signals into a single integrated output.
X-ray Laser Experiment Could Help in Designing Drugs for Brain Disorders
Scientists found that when two protein structures in the brain join up, they act as an amplifier for a slight increase in calcium concentration, triggering a gunshot-like release of neurotransmitters from one neuron to another.
Team Identifies Structure of Tumor-Suppressing Protein
An international group of researchers led by Carnegie Mellon University physicists Mathias Lösche and Frank Heinrich have established the structure of an important tumor suppressing protein, PTEN.
Scroll Up
Scroll Down

Micro-Volume Purity Assessment of Nucleic Acids using A260/A280 Ratio and Spectral Scanning
Bookmark and Share

BioTek Instruments

The application note highlights the ability to determine the concentration and purity of analyte in a sample volume as low as 2 μL using spectrophotometry. Additionally, examples of the ability to perform absorption spectral scans on low volume samples are provided. The spectral data can be useful for detection of common residual contaminants from biomolecule isolation methods by comparison to spectra of highly purified sample.

While the usefulness of the A260/A280 ratio for determining the purity of the sample is understood, it is important to recognize the limitations of relying on the A260/A280 ratio as a sole means for validation.

Further Information

Related Content

BioTek Announces Establishment of Japanese Office
BioTek Japan will be fully functional by July 1, 2013.
Monday, June 10, 2013
BioTek Receives European Patent for Microplate Washer Technology
Ultrasonic Advantage uses unique, integrated ultrasonic cleaning to reduce protein and salt crystal build-up.
Tuesday, June 12, 2012
BioTek Expands Asia Pacific Office
BioTek Instruments, Inc., continues their commitment to the Asia Pacific region with the expansion of their Singapore office.
Thursday, May 10, 2012
BioTek Inducted into Deane C. Davis Vermont Business Hall of Fame
BioTek President/CEO Briar Alpert and Vice President Adam Alpert jointly accepted the award on behalf of BioTek.
Tuesday, June 14, 2011

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,600+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,800+ scientific videos