Corporate Banner
Satellite Banner
Spectroscopy
Scientific Community
 
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
Revealing the Secrets of 19th Century Fashion Industry
The dye industry of the 19th century was fast-moving and international, according to a state-of-the-art analysis of four purple dresses.
How Nanoparticles Damage Immune Cells
New evidence points to protein oxidation, a common means of molecular damage.
Single Molecule Detection of Contaminants, Explosives or Diseases
A technique that combines the ultrasensitivity of surface-enhanced Raman scattering (SERS) with a slippery surface invented by Penn State researchers will make it feasible to detect single molecules of a number of chemical and biological species from gaseous, liquid or solid samples.
Extracting Uranium from Seawater
An ultra-high-resolution technique used for the first time to study polymer fibers that trap uranium in seawater may cause researchers to rethink the best methods to harvest this potential fuel for nuclear reactors.
Innovation Boosts Study of Fragile Biological Samples
Researchers have found a simple new way to study very delicate biological samples – like proteins at work in photosynthesis and components of protein-making machines called ribosomes – at the atomic scale using SLAC's X-ray laser.
Clues for Battling Botulism
Scientists decipher details of deadly toxin's cloaking mechanism that could guide development of new vaccines, treatments.
The US ARL in Maryland Combines Raman Spectroscopy and AFM
Characterizing electrochemical energy storage materials.
Closing the Loop on an HIV Escape Mechanism
Research team finds that protein motions regulate virus infectivity.
New Analysis Technique for Chiral Activity in Molecules
Professor Hyunwoo Kim of the Chemistry Department and his research team have developed a technique that can easily analyze the optical activity of charged compounds by using nuclear magnetic resonance (NMR) spectroscopy.
Miniaturizable Magnetic Resonance
Microscopic gem the key to new development in magnetic lab-on-a-chip technology.
Scroll Up
Scroll Down

Determination of Antioxidant potential using an Oxygen Radical Absorbance Capacity (ORAC) Assay with Synergy™ H4
Bookmark and Share

BioTek Instruments

Oxidative damage to living organisms has been associated with several disease states as well as aging. The sources of reactive oxygen species (ROS) are varied and can lead to the formation of toxic compounds within organisms. ROS sources such as cellular respiration, interaction of biomolecules with ionizing radiation, and dedicated cellular pathways for ROS formation as a protective mechanism ensure chronic exposure of living organisms to ROS species.

A proper balance must be maintained between oxidants and antioxidants to ensure the ubiquitous ROS species do not become deleterious to the organism. Disruption of any one of these mechanisms can result in an imbalance and ensuing damage to a number of critical components required to maintain cellular homeostasis. Thus, there is interest in the ability to accurately determine the antioxidant capabilities of foods, cosmetics, dietary supplements and pharmaceutical agents.

While several methods exist to measure total antioxidant capacity, ORAC has emerged as a low cost method suitable for high throughput automation in a microplate format. The ORAC assay relies on free radical damage to a fluorescent probe, most commonly fluorescein, caused by an oxidizing reagent resulting in a loss of fluorescent intensity over time.The resultant damage can then be correlated with the amount of oxidant present. Conversely, inhibition of oxidative damage to the fluorescent probe can be correlated with the antioxidant capacity of a compound acting as a free radical scavenger.

This application note demonstrates the ability to perform the assay in a 96-well microplate format amenable to higher throughput platforms.

Further Information

Related Content

BioTek Announces Establishment of Japanese Office
BioTek Japan will be fully functional by July 1, 2013.
Monday, June 10, 2013
BioTek Receives European Patent for Microplate Washer Technology
Ultrasonic Advantage uses unique, integrated ultrasonic cleaning to reduce protein and salt crystal build-up.
Tuesday, June 12, 2012
BioTek Expands Asia Pacific Office
BioTek Instruments, Inc., continues their commitment to the Asia Pacific region with the expansion of their Singapore office.
Thursday, May 10, 2012
BioTek Inducted into Deane C. Davis Vermont Business Hall of Fame
BioTek President/CEO Briar Alpert and Vice President Adam Alpert jointly accepted the award on behalf of BioTek.
Tuesday, June 14, 2011
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,900+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,200+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!