Sunday, May 27, 2012
Technology Networks
Technology Networks
 |  |  |
|
|
 | From Acquisition to Analysis: Tools to Improve the High Content Screening Workflow for Stem Cell Assays Ben Haworth, Molecular Devices, speaking at Screening Europe 2011. |
 | Epithelial-to-mesenchymal Transition Leads to the Acquisition of Cancer Stem Cell Characteristics in Liver Cancer Bart Rountree, Assistant Professor, Penn State College of Medicine speaking at Stem Cells World Congress 2011 |
Cord Blood, Embryonic and Adult Stem Cells Applications & Technologies - A Global Market Watch, 2009-2015
Axis Research Mind
The report reviews the latest stem cells market trends with a perceptive to disclose the near-future growth prospects. An in-depth analysis across the geographic regions provides strategic business intelligence for life science and as well as healthcare sector investments.
Cryopreservation Equipments in Stem Cells - A Global Market Watch, 2009-2015
Axis Research Mind
The report reviews the latest trends in cryopreservation equipments used in stem cells technology with a perceptive to disclose the near-future growth prospects in cryopreservation of stem cells.
Stem Cell Research Market to 2017
GBI Research
Strong Pipeline, High Unmet Needs in Chronic Diseases and Favorable Government Policies to Boost Stem Cell R&D
Stem Cells in Drug Discovery Trends 2011
HTStec
This market report summarizes the results of HTStec’s industry-wide global web-based benchmarking survey on stem cells in drug discovery carried out in June 2011.
3D Cell Culture Trends
HTStec
This market report summarizes the results of HTStec’s second industry-wide global web-based benchmarking survey on three dimensional (3D) cell culture carried out in October 2011.
Circulating Tumor Cells (CTCs) and Cancer Stem Cells (CSCs) Market Report 2012
Select Biosciences Ltd
In this report, we focus upon the biology of CTCs and CSCs as well as explore the inter-relationship between these cell types based upon molecular events. Also presented are research trends which are for the isolation and study of CTCs, a potentially important tool for cancer diagnostics with special relevance to cancer metastatic mechanisms.
Circulating Tumor Cells (CTCs) Market Trends 2011
Select Biosciences Ltd
This is the latest and most up-to-date Market Report from Select Biosciences addressing the circulating tumor cells (CTCs) space. In this report, we focus upon the emerging marketplace for the isolation and study of CTCs, a potentially important tool for cancer diagnostics with special relevance to cancer metastasis and mechanisms thereof.
Umbilical Cord Blood Banking Market Trends 2011
Select Biosciences Ltd
This report focuses upon cord blood banking and provided quantitative market analysis of the space as it relates to the growth of the field, utilization trends of cord blood for different diseases in different countries of the world.
Single Cell Omics 2011
Select Biosciences Ltd
The single cell Omics revolution is the potential transformation of cellular heterogeneity from a source of noise to a source of new, as yet hidden discoveries.
Future Directions in Regenerative Medicine
Business Insights
This report covers the development of therapies to replace human cells or promote their regeneration: including cell therapies, tissue engineering, drugs, and medical devices. Each company profiled has at least one project in preclinical or Phase I study. Many also have later stage or launched products, which are covered briefly to illustrate the potential of the next generation of candidates.
Axis Research Mind
The report reviews the latest stem cells market trends with a perceptive to disclose the near-future growth prospects. An in-depth analysis across the geographic regions provides strategic business intelligence for life science and as well as healthcare sector investments.
Cryopreservation Equipments in Stem Cells - A Global Market Watch, 2009-2015
Axis Research Mind
The report reviews the latest trends in cryopreservation equipments used in stem cells technology with a perceptive to disclose the near-future growth prospects in cryopreservation of stem cells.
Stem Cell Research Market to 2017
GBI Research
Strong Pipeline, High Unmet Needs in Chronic Diseases and Favorable Government Policies to Boost Stem Cell R&D
Stem Cells in Drug Discovery Trends 2011
HTStec
This market report summarizes the results of HTStec’s industry-wide global web-based benchmarking survey on stem cells in drug discovery carried out in June 2011.
3D Cell Culture Trends
HTStec
This market report summarizes the results of HTStec’s second industry-wide global web-based benchmarking survey on three dimensional (3D) cell culture carried out in October 2011.
Circulating Tumor Cells (CTCs) and Cancer Stem Cells (CSCs) Market Report 2012
Select Biosciences Ltd
In this report, we focus upon the biology of CTCs and CSCs as well as explore the inter-relationship between these cell types based upon molecular events. Also presented are research trends which are for the isolation and study of CTCs, a potentially important tool for cancer diagnostics with special relevance to cancer metastatic mechanisms.
Circulating Tumor Cells (CTCs) Market Trends 2011
Select Biosciences Ltd
This is the latest and most up-to-date Market Report from Select Biosciences addressing the circulating tumor cells (CTCs) space. In this report, we focus upon the emerging marketplace for the isolation and study of CTCs, a potentially important tool for cancer diagnostics with special relevance to cancer metastasis and mechanisms thereof.
Umbilical Cord Blood Banking Market Trends 2011
Select Biosciences Ltd
This report focuses upon cord blood banking and provided quantitative market analysis of the space as it relates to the growth of the field, utilization trends of cord blood for different diseases in different countries of the world.
Single Cell Omics 2011
Select Biosciences Ltd
The single cell Omics revolution is the potential transformation of cellular heterogeneity from a source of noise to a source of new, as yet hidden discoveries.
Future Directions in Regenerative Medicine
Business Insights
This report covers the development of therapies to replace human cells or promote their regeneration: including cell therapies, tissue engineering, drugs, and medical devices. Each company profiled has at least one project in preclinical or Phase I study. Many also have later stage or launched products, which are covered briefly to illustrate the potential of the next generation of candidates.
Neurons from Stem Cells Could Replace Mice in Botulinum Test
UW-Madison researchers devised an effective assay for detecting botulinum neurotoxin.
NYSCF Scientist Grows Bone from Human Embryonic Stem Cells
Bone cell progenitors derived from human embryonic stem cells used to grow compact bone tissue in large quantities.
New Technique Reveals Unseen Information in DNA Code
Scientists have developed and tested a technique to distinguish between two modified forms of cytosine.
World's First Approved Stem Cell Drug
Osiris Receives Marketing Clearance from Health Canada for Prochymal. Historic decision offers hope to children suffering from life-threatening GvHD.
Genetic Packing: Successful Stem Cell Differentiation Requires DNA Compaction, Study Finds
New research findings show that embryonic stem cells unable to fully compact the DNA inside them cannot complete their primary task: differentiation into specific cell types that give rise to the various types of tissues and structures in the body.
Promethera Biosciences Treats First Patients with Promethera® HepaStem
Phase I/II study designed to evaluate the ability of liver progenitor cells to restore a missing enzyme function in liver-based metabolic diseases.
New Muscular Dystrophy Treatment Approach Developed Using Human Stem Cells
New process makes the production of human muscle cells from stem cells efficient and effective.
Duke Team Turns Scar Tissue into Heart Muscle Without Using Stem Cells
Scientists at Duke University Medical Center have shown the ability to turn scar tissue that forms after a heart attack into heart muscle cells using a new process that eliminates the need for stem cell transplant.
“Housekeeping” Mechanism for Brain Stem Cells Discovered
Researchers at Columbia University Medical Center (CUMC) have identified a molecular pathway that controls the retention and release of the brain’s stem cells.
IBN Discovers Human Neural Stem Cells with Tumor Targeting Ability
The discovery is promising for the future of breast cancer therapy.
UW-Madison researchers devised an effective assay for detecting botulinum neurotoxin.
NYSCF Scientist Grows Bone from Human Embryonic Stem Cells
Bone cell progenitors derived from human embryonic stem cells used to grow compact bone tissue in large quantities.
New Technique Reveals Unseen Information in DNA Code
Scientists have developed and tested a technique to distinguish between two modified forms of cytosine.
World's First Approved Stem Cell Drug
Osiris Receives Marketing Clearance from Health Canada for Prochymal. Historic decision offers hope to children suffering from life-threatening GvHD.
Genetic Packing: Successful Stem Cell Differentiation Requires DNA Compaction, Study Finds
New research findings show that embryonic stem cells unable to fully compact the DNA inside them cannot complete their primary task: differentiation into specific cell types that give rise to the various types of tissues and structures in the body.
Promethera Biosciences Treats First Patients with Promethera® HepaStem
Phase I/II study designed to evaluate the ability of liver progenitor cells to restore a missing enzyme function in liver-based metabolic diseases.
New Muscular Dystrophy Treatment Approach Developed Using Human Stem Cells
New process makes the production of human muscle cells from stem cells efficient and effective.
Duke Team Turns Scar Tissue into Heart Muscle Without Using Stem Cells
Scientists at Duke University Medical Center have shown the ability to turn scar tissue that forms after a heart attack into heart muscle cells using a new process that eliminates the need for stem cell transplant.
“Housekeeping” Mechanism for Brain Stem Cells Discovered
Researchers at Columbia University Medical Center (CUMC) have identified a molecular pathway that controls the retention and release of the brain’s stem cells.
IBN Discovers Human Neural Stem Cells with Tumor Targeting Ability
The discovery is promising for the future of breast cancer therapy.
Karolinska University Hospital and GE Healthcare Collaborate
Pioneering three-year study will investigate technology and workflow needs in a clinical setting.
BioTime Completes Merger of XenneX, Inc. into LifeMap Sciences, Inc.
LifeMap Sciences now holds the exclusive, worldwide licenses to market GeneCards(R) and PanDaTox from Yeda Research and Development Company Ltd.
GenDx Obtains World Wide Distribution Rights of QIAGEN® LongRange PCR Kit
Performance of GenDx SBTexcellerator® reagents for tissue typing diagnostics in combination with LongRange PCR kit offers best HLA-typing results.
Mesoblast Receives FDA Clearance for Phase 2 Clinical Trial of Proprietary Stem Cell Therapy in Type 2 Diabetes
Trial will be randomized, placebo-controlled to evaluate safety and effectiveness over 3 months of a single intravenous injection of one of three progressively increasing MPC doses in 60 Type 2 diabetes patients with elevated blood glucose levels.
Technology Strategy Board Appoints Cell Therapy Catapult CEO
Mr Keith Thompson will be responsible for short and long-term objectives of the Catapult.
Reinnervate and Roslin Cellab Announce Plans to Collaborate
Collaboration on the development of protocols for three dimensional growth of hESC’s using Alvetex®Scaffold.
VistaGen Secures Key U.S. Stem Cell Technology Patent
United States patent covers the company's proprietary methods used to measure and type the toxic effects produced by drug compounds in liver stem cells.
Threshold Pharmaceuticals Appoints Senior Vice President of Regulatory Affairs
Robert Simon will oversee Threshold's regulatory activities related to the development of TH-302, a clinical stage hypoxia-targeted cancer therapeutic currently being evaluated in a variety of cancers.
Cryo-Save Hires Stem Cell Expert at Flagship Lab
Dr. Jurga will supervise new process validation at the Cryo-Save labs and study new processing techniques.
Intervertebral Disc Repair Phase 2 Trial Update
Rapid patient accrual, enrolment reaches half way mark positive preclinical study results published in premier neurosurgical journal.
Pioneering three-year study will investigate technology and workflow needs in a clinical setting.
BioTime Completes Merger of XenneX, Inc. into LifeMap Sciences, Inc.
LifeMap Sciences now holds the exclusive, worldwide licenses to market GeneCards(R) and PanDaTox from Yeda Research and Development Company Ltd.
GenDx Obtains World Wide Distribution Rights of QIAGEN® LongRange PCR Kit
Performance of GenDx SBTexcellerator® reagents for tissue typing diagnostics in combination with LongRange PCR kit offers best HLA-typing results.
Mesoblast Receives FDA Clearance for Phase 2 Clinical Trial of Proprietary Stem Cell Therapy in Type 2 Diabetes
Trial will be randomized, placebo-controlled to evaluate safety and effectiveness over 3 months of a single intravenous injection of one of three progressively increasing MPC doses in 60 Type 2 diabetes patients with elevated blood glucose levels.
Technology Strategy Board Appoints Cell Therapy Catapult CEO
Mr Keith Thompson will be responsible for short and long-term objectives of the Catapult.
Reinnervate and Roslin Cellab Announce Plans to Collaborate
Collaboration on the development of protocols for three dimensional growth of hESC’s using Alvetex®Scaffold.
VistaGen Secures Key U.S. Stem Cell Technology Patent
United States patent covers the company's proprietary methods used to measure and type the toxic effects produced by drug compounds in liver stem cells.
Threshold Pharmaceuticals Appoints Senior Vice President of Regulatory Affairs
Robert Simon will oversee Threshold's regulatory activities related to the development of TH-302, a clinical stage hypoxia-targeted cancer therapeutic currently being evaluated in a variety of cancers.
Cryo-Save Hires Stem Cell Expert at Flagship Lab
Dr. Jurga will supervise new process validation at the Cryo-Save labs and study new processing techniques.
Intervertebral Disc Repair Phase 2 Trial Update
Rapid patient accrual, enrolment reaches half way mark positive preclinical study results published in premier neurosurgical journal.
 | ||||||||||||||||
|
ISMTR-283 Protects Human Bone Marrow Progenitors from the Lethal Effects of Ionizing Radiation
ReachBio LLC
Bone Marrow (BM) is extremely susceptible to radiation damage. BM from matched donors is often used to ‘rescue’ patients following medical radiation for leukemia. However, in the event of accidental/environmental radiation exposure, the ability to identify appropriate donors in a timely manner is rarely possible. Using CFC assays, we evaluated a small molecule compound (ISMTR-283) for its potential to protect the marrow progenitor cells and microenvironment from radiation damage.
Analyzing Molecular Polar Surface Descriptors to Predict Blood-Brain Barrier Permeation
University of Würzburg
Permeation of active drugs across the vascular brain endothelium into the central nervous system (CNS) is controlled by the blood-brain barrier (BBB). Some molecular quantities like polar surface (PS) descriptors are of key interest to medicinal chemists to predict the BBB permeation fate for different drug-like chemical compounds.
The Mesenchymal Stem Cells Bioengineered Tissue Using Cobalt-60 Radiation
IPEN/CNEN - USP
The biological effects of gamma radiation by cobalt-60 sources on epidermal-dermal matrix have not been fully elucidated when mesenchymal stem cells cultured add in. This poster shows how scientists standardized the keratinocytes and adipose-derived stem cell (ADSC) culture procedure before constructing a bioengineered dermal-epidermal equivalent populated by keratinocytes and ADSC cultured cells.
Expression of Pluripotency-determining Factors in in vitro Fertilized Buffalo Embryos and Embryonic Stem Cells
Animal Biotechnology Centre, NDRI
The POU octamer-binding domain transcription factor Oct-4, Stage-specific embryonic antigens (SSEAs), and Tumor rejection antigens (TRAs), are developmentally regulated during early embryogenesis.
Effect of Culture Media and Serum Supplementation on the Development of in vitro Fertilized Buffalo Embryos
Central Institute for Research on Buffaloes
This poster compares the development of buffalo embryos in simple and complex culture media and aims to determine the effects of serum supplementation on the development of buffalo embryos.
High Content Analysis of Neural Stem Cell Expansion and Differentiation
Molecular Devices
Automated assay methods for monitoring neural stem cell expansion and differentiation using stem cell derived neural cell lines and high content imaging systems have been described.
Neurotoxicity Assays Using iPSC-Derived Neurons and High Content Imaging
Molecular Devices
Neurotoxicity can cause temporary or permanent damage of brain or peripheral nervous system and has been found to be a major cause of neurodegenerative diseases such as Alzheimer’s or Parkinson’s. Accordingly, there is a great interest in developing more predictive, disease relevant cell-based models and efficient screening tools for assessing the neurotoxicity of chemical compounds, drug candidates and environmental agents.
Live Cell Beating Assay Using Human iPSC-derived Cardiomyocytes for Evaluation of Drug Efficacy and Toxicity
Molecular Devices
A large percentage of new drugs fail in clinical studies due to cardiac toxicity. Development of highly predictive in vitro assays suitable for screening, safety assessment or other environments is therefore extremely important for drug development. Human cardiomyocytes derived from stem cell sources can greatly accelerate the discovery of cardiac drugs and improve drug safety by offering more clinically relevant cell-based models than those presently available.
Regulation of shoot apical meristem development by SEUSS and SEUSS-LIKE 2 in Arabidopsis
The University of Melbourne
In Arabidopsis, SEU and SLK2 are redundant components of a regulatory complex that is proposed to promote shoot apical meristem (SAM) formation during embryogenesis. Expression analysis indicates that SEU and SLK2 act upstream of several known SAM regulators, and also regulate auxin accumulation, probably via interaction with auxin response factors.
Expansion of mesenchymal stem cells from frozen UCB
StemCare A/S and Bioneer A/S
Umbilical cord blood (UCB) has recently been the focus of clinical applications. UCB contains of hematopoietic stem cells and mesenchymal stem cells (MSC). Clinical studies shows that MSC can be used in regenerative medicine hereby treatment of cardiac diseases. The aim of this project is to establish a protocol for isolation of MSCs from frozen UCB. This study demonstrated that it’s possible to expand MSC.
ReachBio LLC
Bone Marrow (BM) is extremely susceptible to radiation damage. BM from matched donors is often used to ‘rescue’ patients following medical radiation for leukemia. However, in the event of accidental/environmental radiation exposure, the ability to identify appropriate donors in a timely manner is rarely possible. Using CFC assays, we evaluated a small molecule compound (ISMTR-283) for its potential to protect the marrow progenitor cells and microenvironment from radiation damage.
Analyzing Molecular Polar Surface Descriptors to Predict Blood-Brain Barrier Permeation
University of Würzburg
Permeation of active drugs across the vascular brain endothelium into the central nervous system (CNS) is controlled by the blood-brain barrier (BBB). Some molecular quantities like polar surface (PS) descriptors are of key interest to medicinal chemists to predict the BBB permeation fate for different drug-like chemical compounds.
The Mesenchymal Stem Cells Bioengineered Tissue Using Cobalt-60 Radiation
IPEN/CNEN - USP
The biological effects of gamma radiation by cobalt-60 sources on epidermal-dermal matrix have not been fully elucidated when mesenchymal stem cells cultured add in. This poster shows how scientists standardized the keratinocytes and adipose-derived stem cell (ADSC) culture procedure before constructing a bioengineered dermal-epidermal equivalent populated by keratinocytes and ADSC cultured cells.
Expression of Pluripotency-determining Factors in in vitro Fertilized Buffalo Embryos and Embryonic Stem Cells
Animal Biotechnology Centre, NDRI
The POU octamer-binding domain transcription factor Oct-4, Stage-specific embryonic antigens (SSEAs), and Tumor rejection antigens (TRAs), are developmentally regulated during early embryogenesis.
Effect of Culture Media and Serum Supplementation on the Development of in vitro Fertilized Buffalo Embryos
Central Institute for Research on Buffaloes
This poster compares the development of buffalo embryos in simple and complex culture media and aims to determine the effects of serum supplementation on the development of buffalo embryos.
High Content Analysis of Neural Stem Cell Expansion and Differentiation
Molecular Devices
Automated assay methods for monitoring neural stem cell expansion and differentiation using stem cell derived neural cell lines and high content imaging systems have been described.
Neurotoxicity Assays Using iPSC-Derived Neurons and High Content Imaging
Molecular Devices
Neurotoxicity can cause temporary or permanent damage of brain or peripheral nervous system and has been found to be a major cause of neurodegenerative diseases such as Alzheimer’s or Parkinson’s. Accordingly, there is a great interest in developing more predictive, disease relevant cell-based models and efficient screening tools for assessing the neurotoxicity of chemical compounds, drug candidates and environmental agents.
Live Cell Beating Assay Using Human iPSC-derived Cardiomyocytes for Evaluation of Drug Efficacy and Toxicity
Molecular Devices
A large percentage of new drugs fail in clinical studies due to cardiac toxicity. Development of highly predictive in vitro assays suitable for screening, safety assessment or other environments is therefore extremely important for drug development. Human cardiomyocytes derived from stem cell sources can greatly accelerate the discovery of cardiac drugs and improve drug safety by offering more clinically relevant cell-based models than those presently available.
Regulation of shoot apical meristem development by SEUSS and SEUSS-LIKE 2 in Arabidopsis
The University of Melbourne
In Arabidopsis, SEU and SLK2 are redundant components of a regulatory complex that is proposed to promote shoot apical meristem (SAM) formation during embryogenesis. Expression analysis indicates that SEU and SLK2 act upstream of several known SAM regulators, and also regulate auxin accumulation, probably via interaction with auxin response factors.
Expansion of mesenchymal stem cells from frozen UCB
StemCare A/S and Bioneer A/S
Umbilical cord blood (UCB) has recently been the focus of clinical applications. UCB contains of hematopoietic stem cells and mesenchymal stem cells (MSC). Clinical studies shows that MSC can be used in regenerative medicine hereby treatment of cardiac diseases. The aim of this project is to establish a protocol for isolation of MSCs from frozen UCB. This study demonstrated that it’s possible to expand MSC.
Analyzing Biological Samples
Tecan
This technical note describes the capability of Tecan’s Infinite® 200 PRO multimode reader to regulate the CO2 partial pressure inside its measurement chamber.
Measurement of Proteasome Inhibition in Live Cells in Molecular Devices Microplate Fluorometers
Molecular Devices
The proteasome is a massive protein complex which can recognize and bind targeted proteins, and inject them into the central core where the proteins are successively degraded into short peptides. Whereas protein synthesis is well-understood after decades of study, major advances in our knowledge of protein degradation have occurred only in the last two decades.
Isolation and Characterization of Human Bone-Marrow-Derived Mesenchymal Stem Cells
Millipore
Mesenchymal stem cells derived from bone marrow have become an attractive model cell system for the in vitro and in vivo study of disease states such as osteoporosis, obesity, and arthritis. We have isolated mesenchymal stem cells (MSCs) from the bone marrow aspirate of a single human donor. Human bone-marrow-derived MSCs express the mesenchymal stem cell markers STR0-1, CD44, and CD90, and do not express hematopoietic markers CD14 and CD19. Human bone-marrow-derived MSCs can be expanded for 18
Stem Cell-Derived Human Neurons in CNS Drug Discovery
Molecular Devices
Central nervous system (CNS) disorders affect many people worldwide. To increase the quality and number of drugs available for treatments of CNS disorders such as Alzheimer’s disease, schizophrenia and anxiety, the success rate of CNS drug discovery programs must be improved. The challenge in drug discovery is to develop more relevant assay systems to identify lead compounds for further development. Cell-based screening assays currently used for CNS drug discovery and development involve primary
Nucleofection® of mouse ES cells
Amaxa GMBH
Efficient delivery of DNA is crucial for gene targeting in embryonic stem cells and for successful generation of knock-out mice. Data presented in this technote show that Nucleofection® leads to a homogenous gene expression pattern in mouse ES cell colonies. In contrast to standard electroporation, Nucleofection® results in a 100% deletion of frt-flanked genes on both alleles. Viable and fertile mice were generated from transfected mouse ES cell clones.
Tecan
This technical note describes the capability of Tecan’s Infinite® 200 PRO multimode reader to regulate the CO2 partial pressure inside its measurement chamber.
Measurement of Proteasome Inhibition in Live Cells in Molecular Devices Microplate Fluorometers
Molecular Devices
The proteasome is a massive protein complex which can recognize and bind targeted proteins, and inject them into the central core where the proteins are successively degraded into short peptides. Whereas protein synthesis is well-understood after decades of study, major advances in our knowledge of protein degradation have occurred only in the last two decades.
Isolation and Characterization of Human Bone-Marrow-Derived Mesenchymal Stem Cells
Millipore
Mesenchymal stem cells derived from bone marrow have become an attractive model cell system for the in vitro and in vivo study of disease states such as osteoporosis, obesity, and arthritis. We have isolated mesenchymal stem cells (MSCs) from the bone marrow aspirate of a single human donor. Human bone-marrow-derived MSCs express the mesenchymal stem cell markers STR0-1, CD44, and CD90, and do not express hematopoietic markers CD14 and CD19. Human bone-marrow-derived MSCs can be expanded for 18
Stem Cell-Derived Human Neurons in CNS Drug Discovery
Molecular Devices
Central nervous system (CNS) disorders affect many people worldwide. To increase the quality and number of drugs available for treatments of CNS disorders such as Alzheimer’s disease, schizophrenia and anxiety, the success rate of CNS drug discovery programs must be improved. The challenge in drug discovery is to develop more relevant assay systems to identify lead compounds for further development. Cell-based screening assays currently used for CNS drug discovery and development involve primary
Nucleofection® of mouse ES cells
Amaxa GMBH
Efficient delivery of DNA is crucial for gene targeting in embryonic stem cells and for successful generation of knock-out mice. Data presented in this technote show that Nucleofection® leads to a homogenous gene expression pattern in mouse ES cell colonies. In contrast to standard electroporation, Nucleofection® results in a 100% deletion of frt-flanked genes on both alleles. Viable and fertile mice were generated from transfected mouse ES cell clones.
©2012 Technology Networks, all rights reserved.