Thursday, February 23, 2012
Technology Networks
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 | From Acquisition to Analysis: Tools to Improve the High Content Screening Workflow for Stem Cell Assays Ben Haworth, Molecular Devices, speaking at Screening Europe 2011. |
 | Epithelial-to-mesenchymal Transition Leads to the Acquisition of Cancer Stem Cell Characteristics in Liver Cancer Bart Rountree, Assistant Professor, Penn State College of Medicine speaking at Stem Cells World Congress 2011 |
Stem Cells in Drug Discovery Trends 2011
HTStec
This market report summarizes the results of HTStec’s industry-wide global web-based benchmarking survey on stem cells in drug discovery carried out in June 2011.
3D Cell Culture Trends
HTStec
This market report summarizes the results of HTStec’s second industry-wide global web-based benchmarking survey on three dimensional (3D) cell culture carried out in October 2011.
Circulating Tumor Cells (CTCs) and Cancer Stem Cells (CSCs) Market Report 2012
Select Biosciences Ltd
In this report, we focus upon the biology of CTCs and CSCs as well as explore the inter-relationship between these cell types based upon molecular events. Also presented are research trends which are for the isolation and study of CTCs, a potentially important tool for cancer diagnostics with special relevance to cancer metastatic mechanisms.
Circulating Tumor Cells (CTCs) Market Trends 2011
Select Biosciences Ltd
This is the latest and most up-to-date Market Report from Select Biosciences addressing the circulating tumor cells (CTCs) space. In this report, we focus upon the emerging marketplace for the isolation and study of CTCs, a potentially important tool for cancer diagnostics with special relevance to cancer metastasis and mechanisms thereof.
Umbilical Cord Blood Banking Market Trends 2011
Select Biosciences Ltd
This report focuses upon cord blood banking and provided quantitative market analysis of the space as it relates to the growth of the field, utilization trends of cord blood for different diseases in different countries of the world.
Single Cell Omics 2011
Select Biosciences Ltd
The single cell Omics revolution is the potential transformation of cellular heterogeneity from a source of noise to a source of new, as yet hidden discoveries.
Future Directions in Regenerative Medicine
Business Insights
This report covers the development of therapies to replace human cells or promote their regeneration: including cell therapies, tissue engineering, drugs, and medical devices. Each company profiled has at least one project in preclinical or Phase I study. Many also have later stage or launched products, which are covered briefly to illustrate the potential of the next generation of candidates.
Stem Cell Therapy Holds Promise for Ischemic Heart Disease Patients
GlobalData
Data and information on the most commonly reprocessed market assessment of Stem Cell Therapy in cardiovascular disease.
Innovations in Combating Infectious Diseases: Opportunities in therapeutics and diagnostics through application of proteomics, genomics, nanotechnology, and novel sources of lead generation
Business Insights
This report makes a detailed and comprehensive analysis of the cutting edge of research aiming to reveal how bacteria, viruses, fungi, and prions infect and affect their hosts. It also assesses the new technologies and techniques that are being used to design and develop the anti-infective drugs and diagnostic methods of the 21st century.
Stem Cells for Cellular Therapy 2010 Market Report
Select Biosciences Ltd
This market report is focused on presenting the market characteristics of stem cells as they are currently impacting research in the cellular therapy space. Furthermore, this report also addresses an important market, namely that of Umbilical Cord Blood Banking.
HTStec
This market report summarizes the results of HTStec’s industry-wide global web-based benchmarking survey on stem cells in drug discovery carried out in June 2011.
3D Cell Culture Trends
HTStec
This market report summarizes the results of HTStec’s second industry-wide global web-based benchmarking survey on three dimensional (3D) cell culture carried out in October 2011.
Circulating Tumor Cells (CTCs) and Cancer Stem Cells (CSCs) Market Report 2012
Select Biosciences Ltd
In this report, we focus upon the biology of CTCs and CSCs as well as explore the inter-relationship between these cell types based upon molecular events. Also presented are research trends which are for the isolation and study of CTCs, a potentially important tool for cancer diagnostics with special relevance to cancer metastatic mechanisms.
Circulating Tumor Cells (CTCs) Market Trends 2011
Select Biosciences Ltd
This is the latest and most up-to-date Market Report from Select Biosciences addressing the circulating tumor cells (CTCs) space. In this report, we focus upon the emerging marketplace for the isolation and study of CTCs, a potentially important tool for cancer diagnostics with special relevance to cancer metastasis and mechanisms thereof.
Umbilical Cord Blood Banking Market Trends 2011
Select Biosciences Ltd
This report focuses upon cord blood banking and provided quantitative market analysis of the space as it relates to the growth of the field, utilization trends of cord blood for different diseases in different countries of the world.
Single Cell Omics 2011
Select Biosciences Ltd
The single cell Omics revolution is the potential transformation of cellular heterogeneity from a source of noise to a source of new, as yet hidden discoveries.
Future Directions in Regenerative Medicine
Business Insights
This report covers the development of therapies to replace human cells or promote their regeneration: including cell therapies, tissue engineering, drugs, and medical devices. Each company profiled has at least one project in preclinical or Phase I study. Many also have later stage or launched products, which are covered briefly to illustrate the potential of the next generation of candidates.
Stem Cell Therapy Holds Promise for Ischemic Heart Disease Patients
GlobalData
Data and information on the most commonly reprocessed market assessment of Stem Cell Therapy in cardiovascular disease.
Innovations in Combating Infectious Diseases: Opportunities in therapeutics and diagnostics through application of proteomics, genomics, nanotechnology, and novel sources of lead generation
Business Insights
This report makes a detailed and comprehensive analysis of the cutting edge of research aiming to reveal how bacteria, viruses, fungi, and prions infect and affect their hosts. It also assesses the new technologies and techniques that are being used to design and develop the anti-infective drugs and diagnostic methods of the 21st century.
Stem Cells for Cellular Therapy 2010 Market Report
Select Biosciences Ltd
This market report is focused on presenting the market characteristics of stem cells as they are currently impacting research in the cellular therapy space. Furthermore, this report also addresses an important market, namely that of Umbilical Cord Blood Banking.
Current Status and Future Development of Cell Transplantation Therapy for Periodontal Tissue Regeneration
This paper reviews the current research targeting cell-based therapies for periodontal regeneration and then discusses the risks and governmental controls of stem cell transplantation therapy.
American CryoStem Completes Cell Processing for Clinical Study
The study involves researching the regenerative function of participant's adipose derived mesenchymal stem cells and their ability to heal skin wounds.
UA Awarded National Cancer Research Grant
The $600,000 grant will fund the Center for Targeted Cancer Therapies, created in 2002 to discover new therapies to treat pancreatic cancer.
Need Muscle for a Tough Spot? Turn to Fat Stem Cells
Encouraged to develop on a stiff surface, stem cells derived from fat undergo a remarkable transformation toward becoming mature muscle cells. The new research appears in the journal Biomaterials.
Blood-forming Stem Cells' Growth Identified in First Breakthrough from New Institute
Endothelial and perivascular cells are responsible for nurturing haematopoietic stem cells.
Juventas Therapeutics Reports One Year Data from Phase I Heart Failure Clinical Trial
JVS-100, encodes Stromal cell-Derived Factor 1 (SDF-1) which has been shown to repair damaged tissue through recruitment of circulating stem cells to the site of injury, prevention of ongoing cell death and restoration of blood flow.
RBCC Explores New Cures for Cancer, Brain Injuries & Headaches
Rainbow Coral Corp. is hard at work identifying and targeting promising new stem-cell treatments for commercialization.
8,000 Animals Treated With Vet-Stem Cell Therapy
After providing stem cells for thousands of horses, Vet-Stem pioneered stem cell therapy in dogs and cats.
Scientists Use Silk from the Tasar Silkworm as a Scaffold for Heart Tissue
Scientists from the Max Planck Institute for Heart and Lung Research in Bad Nauheim are seeking to restore complete cardiac function with the help of artificial cardiac tissue.
Collaborative Research Sheds Light on New Cancer Stem Cell Therapies
The Hong Kong Polytechnic University (PolyU), Peking University Shenzhen Graduate School and Nevada Cancer Institute have developed a novel class of chemical inhibitors that specifically target cancer cells with pluripotency.
This paper reviews the current research targeting cell-based therapies for periodontal regeneration and then discusses the risks and governmental controls of stem cell transplantation therapy.
American CryoStem Completes Cell Processing for Clinical Study
The study involves researching the regenerative function of participant's adipose derived mesenchymal stem cells and their ability to heal skin wounds.
UA Awarded National Cancer Research Grant
The $600,000 grant will fund the Center for Targeted Cancer Therapies, created in 2002 to discover new therapies to treat pancreatic cancer.
Need Muscle for a Tough Spot? Turn to Fat Stem Cells
Encouraged to develop on a stiff surface, stem cells derived from fat undergo a remarkable transformation toward becoming mature muscle cells. The new research appears in the journal Biomaterials.
Blood-forming Stem Cells' Growth Identified in First Breakthrough from New Institute
Endothelial and perivascular cells are responsible for nurturing haematopoietic stem cells.
Juventas Therapeutics Reports One Year Data from Phase I Heart Failure Clinical Trial
JVS-100, encodes Stromal cell-Derived Factor 1 (SDF-1) which has been shown to repair damaged tissue through recruitment of circulating stem cells to the site of injury, prevention of ongoing cell death and restoration of blood flow.
RBCC Explores New Cures for Cancer, Brain Injuries & Headaches
Rainbow Coral Corp. is hard at work identifying and targeting promising new stem-cell treatments for commercialization.
8,000 Animals Treated With Vet-Stem Cell Therapy
After providing stem cells for thousands of horses, Vet-Stem pioneered stem cell therapy in dogs and cats.
Scientists Use Silk from the Tasar Silkworm as a Scaffold for Heart Tissue
Scientists from the Max Planck Institute for Heart and Lung Research in Bad Nauheim are seeking to restore complete cardiac function with the help of artificial cardiac tissue.
Collaborative Research Sheds Light on New Cancer Stem Cell Therapies
The Hong Kong Polytechnic University (PolyU), Peking University Shenzhen Graduate School and Nevada Cancer Institute have developed a novel class of chemical inhibitors that specifically target cancer cells with pluripotency.
Stemedica Granted US Patent for Cellular Scaffold
Multi-stem cell products address need in the $12.5 billion wound care field.
Plasticell Announces Grant of Fundamental CombiCult™ Patents in USA and Japan
Patents have now been issued in Europe, USA, Japan, Australia and Singapore.
The Gamida Cell-Teva Joint Venture Concludes Enrollment for the Phase III Study of StemEx(R)
The results of the StemEx study, a cord blood stem cell product for leaukemia and lymphoma, are expected during the second half of 2012.
Cytomedix Expands Commitment to Regenerative Medicine with Acquisition of Aldegen
Under the terms of the transaction as described below, Cytomedix issued preferred shares valued at $16 million based on a 10-day volume-weighted average price calculated through February 2, 2012.
Roche’s xCELLigence Cardio Instrument Used to Study Stem Cell-Derived Cardiomyocytes
Instrument detected beating rhythm and proarrhythmic effects of nine different compounds on a monolayer of cells, providing evidence for the potential of future screening assays.
American CryoStem Joins Alliance for Regenerative Medicine
The company’s decision to join ARM was based on the alignment of its ongoing mission to offer the highest quality products and services to help accelerate the regenerative medicine industry and ARM's support of policy efforts toward safe and reliable cellular products.
VistaGen Increases Its Drug Rescue Opportunities
Long-term relationship with global contract research and development organization expands to include right-of-first-offer for potential drug rescue candidates.
Prometheus Signs Research & Collaboration Agreement with Worldwide Pharmaceutical Company
The collaboration will use Prometheus' proprietary oncology diagnostic platform (CEER) to develop diagnostic arrays relating to the partner's oncology pipeline.
Sistemic to Work with TiGenix On Landmark EUR 5.9 Million, FP7 European Project
The collaboration aims at developing an innovative rheumatoid arthritis cell therapy treatment product.
BioRestorative Therapies Signs License Agreement for Stem Cell Disc/Spine Procedure
Patient treatments of bulging and herniated discs show promising results.
Multi-stem cell products address need in the $12.5 billion wound care field.
Plasticell Announces Grant of Fundamental CombiCult™ Patents in USA and Japan
Patents have now been issued in Europe, USA, Japan, Australia and Singapore.
The Gamida Cell-Teva Joint Venture Concludes Enrollment for the Phase III Study of StemEx(R)
The results of the StemEx study, a cord blood stem cell product for leaukemia and lymphoma, are expected during the second half of 2012.
Cytomedix Expands Commitment to Regenerative Medicine with Acquisition of Aldegen
Under the terms of the transaction as described below, Cytomedix issued preferred shares valued at $16 million based on a 10-day volume-weighted average price calculated through February 2, 2012.
Roche’s xCELLigence Cardio Instrument Used to Study Stem Cell-Derived Cardiomyocytes
Instrument detected beating rhythm and proarrhythmic effects of nine different compounds on a monolayer of cells, providing evidence for the potential of future screening assays.
American CryoStem Joins Alliance for Regenerative Medicine
The company’s decision to join ARM was based on the alignment of its ongoing mission to offer the highest quality products and services to help accelerate the regenerative medicine industry and ARM's support of policy efforts toward safe and reliable cellular products.
VistaGen Increases Its Drug Rescue Opportunities
Long-term relationship with global contract research and development organization expands to include right-of-first-offer for potential drug rescue candidates.
Prometheus Signs Research & Collaboration Agreement with Worldwide Pharmaceutical Company
The collaboration will use Prometheus' proprietary oncology diagnostic platform (CEER) to develop diagnostic arrays relating to the partner's oncology pipeline.
Sistemic to Work with TiGenix On Landmark EUR 5.9 Million, FP7 European Project
The collaboration aims at developing an innovative rheumatoid arthritis cell therapy treatment product.
BioRestorative Therapies Signs License Agreement for Stem Cell Disc/Spine Procedure
Patient treatments of bulging and herniated discs show promising results.
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The Mesenchymal Stem Cells Bioengineered Tissue Using Cobalt-60 Radiation
IPEN/CNEN - USP
The biological effects of gamma radiation by cobalt-60 sources on epidermal-dermal matrix have not been fully elucidated when mesenchymal stem cells cultured add in. This poster shows how scientists standardized the keratinocytes and adipose-derived stem cell (ADSC) culture procedure before constructing a bioengineered dermal-epidermal equivalent populated by keratinocytes and ADSC cultured cells.
Expression of Pluripotency-determining Factors in in vitro Fertilized Buffalo Embryos and Embryonic Stem Cells
Animal Biotechnology Centre, NDRI
The POU octamer-binding domain transcription factor Oct-4, Stage-specific embryonic antigens (SSEAs), and Tumor rejection antigens (TRAs), are developmentally regulated during early embryogenesis.
Effect of Culture Media and Serum Supplementation on the Development of in vitro Fertilized Buffalo Embryos
Central Institute for Research on Buffaloes
This poster compares the development of buffalo embryos in simple and complex culture media and aims to determine the effects of serum supplementation on the development of buffalo embryos.
High Content Analysis of Neural Stem Cell Expansion and Differentiation
Molecular Devices
Automated assay methods for monitoring neural stem cell expansion and differentiation using stem cell derived neural cell lines and high content imaging systems have been described.
Neurotoxicity Assays Using iPSC-Derived Neurons and High Content Imaging
Molecular Devices
Neurotoxicity can cause temporary or permanent damage of brain or peripheral nervous system and has been found to be a major cause of neurodegenerative diseases such as Alzheimer’s or Parkinson’s. Accordingly, there is a great interest in developing more predictive, disease relevant cell-based models and efficient screening tools for assessing the neurotoxicity of chemical compounds, drug candidates and environmental agents.
Live Cell Beating Assay Using Human iPSC-derived Cardiomyocytes for Evaluation of Drug Efficacy and Toxicity
Molecular Devices
A large percentage of new drugs fail in clinical studies due to cardiac toxicity. Development of highly predictive in vitro assays suitable for screening, safety assessment or other environments is therefore extremely important for drug development. Human cardiomyocytes derived from stem cell sources can greatly accelerate the discovery of cardiac drugs and improve drug safety by offering more clinically relevant cell-based models than those presently available.
Regulation of shoot apical meristem development by SEUSS and SEUSS-LIKE 2 in Arabidopsis
The University of Melbourne
In Arabidopsis, SEU and SLK2 are redundant components of a regulatory complex that is proposed to promote shoot apical meristem (SAM) formation during embryogenesis. Expression analysis indicates that SEU and SLK2 act upstream of several known SAM regulators, and also regulate auxin accumulation, probably via interaction with auxin response factors.
Expansion of mesenchymal stem cells from frozen UCB
StemCare A/S and Bioneer A/S
Umbilical cord blood (UCB) has recently been the focus of clinical applications. UCB contains of hematopoietic stem cells and mesenchymal stem cells (MSC). Clinical studies shows that MSC can be used in regenerative medicine hereby treatment of cardiac diseases. The aim of this project is to establish a protocol for isolation of MSCs from frozen UCB. This study demonstrated that it’s possible to expand MSC.
A high-throughput colony formation assay for profiling novel compounds and RNAi reagents using the Acumen® eX3
TTP Labtech Ltd
Cell colony formation assays measure a cell's ability to grow unattached to a surface and have applications in a range of areas including hematopoietic stem cell research, cell transformation studies and the prediction of responses of tumors to chemotherapeutic agents. The results of this study demonstrated that Acumen eX3 can be used as a high-throughput platform for investigation of effects of test compounds and RNAi reagents on cell colony formation.
New Culture Medium Creates Immune Tolerance Between Two Allogeneic Tissue Cells
Biomedical Engineering Dept. and Dept. of Medicine &Epidemiology of Vet. Med. of UC Davis
We discovered that in vitro high dose of Progesterone (P) together with FGFa, EGF, VEGF, LPS had dual effect on Liver tissue cells: increased multiplication of Hepatocytes and suppressed all non-parenchymal (NP) liver tissue cells and created immune tolerance between two allogeneic mice Liver tissue
IPEN/CNEN - USP
The biological effects of gamma radiation by cobalt-60 sources on epidermal-dermal matrix have not been fully elucidated when mesenchymal stem cells cultured add in. This poster shows how scientists standardized the keratinocytes and adipose-derived stem cell (ADSC) culture procedure before constructing a bioengineered dermal-epidermal equivalent populated by keratinocytes and ADSC cultured cells.
Expression of Pluripotency-determining Factors in in vitro Fertilized Buffalo Embryos and Embryonic Stem Cells
Animal Biotechnology Centre, NDRI
The POU octamer-binding domain transcription factor Oct-4, Stage-specific embryonic antigens (SSEAs), and Tumor rejection antigens (TRAs), are developmentally regulated during early embryogenesis.
Effect of Culture Media and Serum Supplementation on the Development of in vitro Fertilized Buffalo Embryos
Central Institute for Research on Buffaloes
This poster compares the development of buffalo embryos in simple and complex culture media and aims to determine the effects of serum supplementation on the development of buffalo embryos.
High Content Analysis of Neural Stem Cell Expansion and Differentiation
Molecular Devices
Automated assay methods for monitoring neural stem cell expansion and differentiation using stem cell derived neural cell lines and high content imaging systems have been described.
Neurotoxicity Assays Using iPSC-Derived Neurons and High Content Imaging
Molecular Devices
Neurotoxicity can cause temporary or permanent damage of brain or peripheral nervous system and has been found to be a major cause of neurodegenerative diseases such as Alzheimer’s or Parkinson’s. Accordingly, there is a great interest in developing more predictive, disease relevant cell-based models and efficient screening tools for assessing the neurotoxicity of chemical compounds, drug candidates and environmental agents.
Live Cell Beating Assay Using Human iPSC-derived Cardiomyocytes for Evaluation of Drug Efficacy and Toxicity
Molecular Devices
A large percentage of new drugs fail in clinical studies due to cardiac toxicity. Development of highly predictive in vitro assays suitable for screening, safety assessment or other environments is therefore extremely important for drug development. Human cardiomyocytes derived from stem cell sources can greatly accelerate the discovery of cardiac drugs and improve drug safety by offering more clinically relevant cell-based models than those presently available.
Regulation of shoot apical meristem development by SEUSS and SEUSS-LIKE 2 in Arabidopsis
The University of Melbourne
In Arabidopsis, SEU and SLK2 are redundant components of a regulatory complex that is proposed to promote shoot apical meristem (SAM) formation during embryogenesis. Expression analysis indicates that SEU and SLK2 act upstream of several known SAM regulators, and also regulate auxin accumulation, probably via interaction with auxin response factors.
Expansion of mesenchymal stem cells from frozen UCB
StemCare A/S and Bioneer A/S
Umbilical cord blood (UCB) has recently been the focus of clinical applications. UCB contains of hematopoietic stem cells and mesenchymal stem cells (MSC). Clinical studies shows that MSC can be used in regenerative medicine hereby treatment of cardiac diseases. The aim of this project is to establish a protocol for isolation of MSCs from frozen UCB. This study demonstrated that it’s possible to expand MSC.
A high-throughput colony formation assay for profiling novel compounds and RNAi reagents using the Acumen® eX3
TTP Labtech Ltd
Cell colony formation assays measure a cell's ability to grow unattached to a surface and have applications in a range of areas including hematopoietic stem cell research, cell transformation studies and the prediction of responses of tumors to chemotherapeutic agents. The results of this study demonstrated that Acumen eX3 can be used as a high-throughput platform for investigation of effects of test compounds and RNAi reagents on cell colony formation.
New Culture Medium Creates Immune Tolerance Between Two Allogeneic Tissue Cells
Biomedical Engineering Dept. and Dept. of Medicine &Epidemiology of Vet. Med. of UC Davis
We discovered that in vitro high dose of Progesterone (P) together with FGFa, EGF, VEGF, LPS had dual effect on Liver tissue cells: increased multiplication of Hepatocytes and suppressed all non-parenchymal (NP) liver tissue cells and created immune tolerance between two allogeneic mice Liver tissue
Analyzing Biological Samples
Tecan
This technical note describes the capability of Tecan’s Infinite® 200 PRO multimode reader to regulate the CO2 partial pressure inside its measurement chamber.
Measurement of Proteasome Inhibition in Live Cells in Molecular Devices Microplate Fluorometers
Molecular Devices
The proteasome is a massive protein complex which can recognize and bind targeted proteins, and inject them into the central core where the proteins are successively degraded into short peptides. Whereas protein synthesis is well-understood after decades of study, major advances in our knowledge of protein degradation have occurred only in the last two decades.
Isolation and Characterization of Human Bone-Marrow-Derived Mesenchymal Stem Cells
Millipore
Mesenchymal stem cells derived from bone marrow have become an attractive model cell system for the in vitro and in vivo study of disease states such as osteoporosis, obesity, and arthritis. We have isolated mesenchymal stem cells (MSCs) from the bone marrow aspirate of a single human donor. Human bone-marrow-derived MSCs express the mesenchymal stem cell markers STR0-1, CD44, and CD90, and do not express hematopoietic markers CD14 and CD19. Human bone-marrow-derived MSCs can be expanded for 18
Stem Cell-Derived Human Neurons in CNS Drug Discovery
Molecular Devices
Central nervous system (CNS) disorders affect many people worldwide. To increase the quality and number of drugs available for treatments of CNS disorders such as Alzheimer’s disease, schizophrenia and anxiety, the success rate of CNS drug discovery programs must be improved. The challenge in drug discovery is to develop more relevant assay systems to identify lead compounds for further development. Cell-based screening assays currently used for CNS drug discovery and development involve primary
Nucleofection® of mouse ES cells
Amaxa GMBH
Efficient delivery of DNA is crucial for gene targeting in embryonic stem cells and for successful generation of knock-out mice. Data presented in this technote show that Nucleofection® leads to a homogenous gene expression pattern in mouse ES cell colonies. In contrast to standard electroporation, Nucleofection® results in a 100% deletion of frt-flanked genes on both alleles. Viable and fertile mice were generated from transfected mouse ES cell clones.
Tecan
This technical note describes the capability of Tecan’s Infinite® 200 PRO multimode reader to regulate the CO2 partial pressure inside its measurement chamber.
Measurement of Proteasome Inhibition in Live Cells in Molecular Devices Microplate Fluorometers
Molecular Devices
The proteasome is a massive protein complex which can recognize and bind targeted proteins, and inject them into the central core where the proteins are successively degraded into short peptides. Whereas protein synthesis is well-understood after decades of study, major advances in our knowledge of protein degradation have occurred only in the last two decades.
Isolation and Characterization of Human Bone-Marrow-Derived Mesenchymal Stem Cells
Millipore
Mesenchymal stem cells derived from bone marrow have become an attractive model cell system for the in vitro and in vivo study of disease states such as osteoporosis, obesity, and arthritis. We have isolated mesenchymal stem cells (MSCs) from the bone marrow aspirate of a single human donor. Human bone-marrow-derived MSCs express the mesenchymal stem cell markers STR0-1, CD44, and CD90, and do not express hematopoietic markers CD14 and CD19. Human bone-marrow-derived MSCs can be expanded for 18
Stem Cell-Derived Human Neurons in CNS Drug Discovery
Molecular Devices
Central nervous system (CNS) disorders affect many people worldwide. To increase the quality and number of drugs available for treatments of CNS disorders such as Alzheimer’s disease, schizophrenia and anxiety, the success rate of CNS drug discovery programs must be improved. The challenge in drug discovery is to develop more relevant assay systems to identify lead compounds for further development. Cell-based screening assays currently used for CNS drug discovery and development involve primary
Nucleofection® of mouse ES cells
Amaxa GMBH
Efficient delivery of DNA is crucial for gene targeting in embryonic stem cells and for successful generation of knock-out mice. Data presented in this technote show that Nucleofection® leads to a homogenous gene expression pattern in mouse ES cell colonies. In contrast to standard electroporation, Nucleofection® results in a 100% deletion of frt-flanked genes on both alleles. Viable and fertile mice were generated from transfected mouse ES cell clones.
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