Corporate Banner
Satellite Banner
Stem Cells, Cellular Therapy & Biobanking
>
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Roche’s xCELLigence Cardio Instrument Used to Study Stem Cell-Derived Cardiomyocytes

Published: Monday, February 13, 2012
Last Updated: Monday, February 13, 2012
Bookmark and Share
Instrument detected beating rhythm and proarrhythmic effects of nine different compounds on a monolayer of cells, providing evidence for the potential of future screening assays.

In an effort to improve preclinical cardiotoxicity assays, reduce drug testing attrition rates, and ensure drug safety, collaborating scientists at the University Medical Center Utrecht in the Netherlands, and Bioscience Department of AstraZeneca R&D, Mölndal, Sweden, have tested Roche’s xCELLigence Cardio Instrument. Their goal was to determine whether impedance recordings are a useful way to detect compound effects on beating frequency of cardiomyocytes, derived either from human induced pluripotent stem cells (hiPS), or from mouse embryonic stem cells (mESC). The xCELLigence Cardio Instrument is an impedance sensing instrument capable of reading signals at high sampling rates, making it possible to measure the contraction movements of cardiomyocytes in contact with sensor microelectrodes.

In this study (1), the effects of nine compounds were tested on beating frequency (beats per minute, bpm) of hiPS and mESC cardiomyocytes. The authors reported, “The results of this initial study show that, under the right conditions, the beating frequency of a monolayer of cells can be stably recorded over several days. In addition, the xCELLigence System detects changes in beating frequency and amplitude caused by added reference compounds.”

The authors conclude that xCELLigence Cardio instrument has potential for 96-well-throughput cardiotoxicity screening of the effects of compounds on rhythmic beating patterns of cardiomyocytes. They underscored the need for continuous improvements in the maturation of available cardiomyocytes and in further validation of the assay on an extended set of reference compounds with known in-vivo effects.  They also indicated that the production of distinct subtypes of ventricular, atrial and nodal cardiomyocytes could open up new areas of screening for arrhythmia and cardiotoxicity.

(1) Malin K.B. Jonsson, Qing-Dong Wang, Bruno Becker: Impedance-Based Detection of Beating Rhythm and Proarrhythmic Effects of Compounds on Stem Cell-Derived Cardiomyocytes.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,000+ scientific posters on ePosters
  • More than 4,500+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

First European Laboratory Obtains Accreditation for New Tissue-Typing Method for Stem Cell Transplants
Tests based on next-generation sequencing with Roche’s GS Junior System.
Thursday, February 28, 2013
Birmingham hospital uses Roche automation solution at blood sciences laboratory
Roche’s automated track system is being used at Queen Elizabeth Hospital to streamline processes and improve turnaround times.
Saturday, June 02, 2012
Roche’s new RTCA Cardio Instrument for Label-Free Functional Cardiomyocyte Toxicity Testing
New medium-throughput cell analyzer utilizes impedance readings to monitor cardiac beating and cellular events in real time.
Wednesday, September 29, 2010
Roche Applied Science Introduces new xCELLigence RTCA MP Instrument
The xCELLigence RTCA MP allows high throughput online-measurement of cell activities without labeling.
Friday, December 05, 2008
454 Sequencing at the Forefront of Bone Marrow and Stem Cell Therapy Research
Roche Genome Sequencer FLX System will create new opportunities for HLA research at the Blood Centre Linz, Austria.
Monday, June 30, 2008
Scientific News
AACR 2016: Cancer Immunotherapy and Beyond
At this year's meeting there was a palpable buzz around subjects ranging from microbiomics to the tumor microenvironment and cancer vaccines, big data to in vitro and in vivo modeling and drug delivery (to name just a few).
How Skeletal Stem Cells Form The Blueprint Of The Face
USC researchers discover that two types of molecular signals work to control where and when stem cells turn into facial cartilage.
Turning Skin Cells into Heart, Brain Cells
In a major breakthrough, scientists at the Gladstone Institutes transformed skin cells into heart cells and brain cells using a combination of chemicals.
Stem Cells Know How to Unwind
Research led by the Babraham Institute with collaborators in the UK, Canada and Japan has revealed a new understanding of how an open genome structure supports the long-term and unrestricted developmental potential in embryonic stem cells.
Growing Stem Cells More Safely
Nurturing stem cells atop a bed of mouse cells works well, but is a non-starter for transplants to patients – Brown University scientists are developing a synthetic bed instead.
Cell Transplant Treats Parkinson’s in Mice
A University of Wisconsin—Madison neuroscientist has inserted a genetic switch into nerve cells so a patient can alter their activity by taking designer drugs that would not affect any other cell.
Skin Cells Turned into Heart Cells and Brain Cells Using Drugs
In a scientific first, Gladstone researchers have used chemical drugs to convert skin cells into heart cells and brain cells, without adding any external genes.
Shape Of Tumor May Affect Whether Cells Can Metastasize
Illinois researchers found that the shape of a tumor may play a role in how cancer cells become primed to spread.
‘Mini-Brains’ to Study Zika
Novel tool expected to speed research on brain and drug development.
Cytokine Triggers Immune Response at Expense of Blood Renewal
Research highlights promise of Anti-IL-1 drugs to treat chronic inflammatory disease.
SELECTBIO

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,000+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,500+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!