Corporate Banner
Satellite Banner
Stem Cells, Cellular Therapy & Biobanking
>
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Stable Cell Line Generation Using Lentivirus

Published: Tuesday, May 29, 2012
Last Updated: Tuesday, May 29, 2012
Bookmark and Share
Fast service for high quality, affordable over-expressing or knockdown stable cell lines.

AMSBIO has announced a fast service for custom generation of high quality, affordable over-expressing or knockdown stable cell lines.

AMSBIO's custom generation of stable cell lines includes cloning the gene or shRNA of interest into a safe lentiviral vector, generating the lentivirus and transducing into the cell line of users choice.

The service allows users to choose a constitutive or inducible promoter, a tag, an antibiotic selection marker and a fluorescent marker.

The ease of providing users target templates or choosing one from AMSBIO's cDNA collection ensures rapid turnaround.

With many years of experience the service can also provide mutagenesis and other special requests.

The stably transduced, high expression cells are then selected, target integration is validated by genomic PCR, and the high-expression clone by Western Blot.

Each customer receives two cryogenic preserved vials of stable cells and a final report.

Working closely with customers - AMSBIO's handles each stage of the process to ensure the delivery of high quality cells that are ready to use. AMSBIO's unique technology ensures a turnaround time as fast as 2 months for each cell line creation.

The AMSBIO lentiviral system is a gene delivery tool for gene expression or knockdown.

Lentivirus can effectively transduce both dividing and non-dividing mammalian cells, and integrate into the host genome, allowing stable long-term, high-level gene expression both in vivo and in vitro.

AMSBIO's stable cell line generation service only use safe (self-inactivating) lentiviral particles to deliver users gene into a wide range of cell lines including primary and stem cells.

Lentiviruses have been used to successfully generate a line of DNA repair knock-down cell lines that AMSBIO offers off the shelf from its product range.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,500+ scientific posters on ePosters
  • More than 5,000+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Novel Organoid Matrix Enables Long-Term Culture of Human Hepatocytes
Organoid growth matrix to enable long-term culture of genome-stable bipotent stem cells from adult human liver.
Saturday, January 31, 2015
Webinar Addresses 3D Cell-Based Models for Regenerative Medicine
Dr Elad Katz presents new on-demand webinar.
Friday, October 25, 2013
Scientific News
Stem Cell ‘Heart Patch’ Almost Perfected
Scientists aiming to perfect and test 3D "heart patches" in animal model, last hurdle before human patients.
Stem Cells Growing 3D Lung-in-a-Dish
Researchers have created 3D lung-like tissue from lung-derived stem cells. The tissue can be used to study lung diseases.
MRI Guidance Aids Stem Cell Delivery
Scientists have delivered stem cells to the brain with unprecedented precision, infusing the cells under real-time MRI guidance.
Mechanisms of Parkinson’s Pathology
Defects that lead to cells’ failure to decommission faulty mitochondria cause nerve cells to die, triggering the symptoms of Parkinson’s disease.
Stem Cell Transplant Without Radiation or Chemotherapy
Researchers have successfully performed stem cell transplants without using radiation or chemotherapy.
Advanced Lymphoma in Remission After T-Cell Therapy
63% of trial participants who recieved two-drug combination chemo plus intermediate dose of engineered T cells went into complete remission.
Inherited Heart Condition Breakthrough
Using stem cells, scientists have created a specific heart condition model, yeilding insights into unexpected disease mechanisms.
Biobank Storage Time Affects Blood Test Results
Study finds storage time of blood samples at a biobank may affect test results as much as patient age.
Transplanted Stem Cells Age Cells by 30 Years
Research suggests stem cell transplants are linked to an increase in immune cell's “molecular age” from blood cancer patients.
Commanding Stem Cells to Build Bone
Natural molecule can coax stem cells into regenerating bone tissue, researchers have discovered.
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,000+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!