Corporate Banner
Satellite Banner
Stem Cells, Cellular Therapy & Biobanking
Scientific Community
Become a Member | Sign in
Home>News>This Article

Merck Millipore Launches MILLIPLEX® MAP Kits

Published: Tuesday, May 07, 2013
Last Updated: Tuesday, May 07, 2013
Bookmark and Share
Enables detection of multiple pluripotency markers in a single measurement.

Merck Millipore has announced availability of the MILLIPLEX® MAP Human Stem Cell Pluripotency Magnetic Bead Kits, which enable the analysis of many different pluripotency biomarkers in a single measurement.

These kits are the first commercially available multiplexed protein biomarker assays based on the Luminex® xMAP® platform for this important research area.

This new application of trusted bead-based multiplex technology gives researchers rapid feedback on the potency status of stem cell cultures and offers a significant advantage over existing techniques, which detect a limited number of proteins with a semi-quantitative output for a single sample.

Merck Millipore's new MILLIPLEX® MAP kits examine protein biomarkers that indicate the potency of stem cells, enabling pluripotent stem cells to be distinguished from multipotent or differentiating cells.

These new kits save time and resources by accurately detecting large numbers of pluripotency markers in one measurement. Kits can be used on cultures of induced pluripotent stem cells, human embryonic stem cells, cancer stem cells or cancer cell lines.

Two kits are available for assessing stem cell pluripotency. The MILLIPLEX® MAP Human Stem Cell Pluripotency Magnetic Bead Kit 1 monitors the expression of the transcription factors Oct 3/4, Sox2, Nanog and c-Myc, which play critical roles in the maintenance of pluripotency and self-renewal in stem cells.

The MILLIPLEX® MAP Human Stem Cell Pluripotency Magnetic Bead Kit 2 contains seven additional pluripotency-related markers, including a transcription factor, a translational regulator, transmembrane proteins and cell surface glycostructures.

These assays are essential for assessing the potency of stem cell cultures in studies ranging from cancer and developmental biology research to bioprocess monitoring. MILLIPLEX® MAP assays make it easy to analyze both cell surface and intracellular proteins.

"The MILLIPLEX® MAP Stem Cell Pluripotency Kit 1 is a valuable tool for stem cell research. We obtained very interesting and promising results," said Frank Edenhofer, Ph.D., Head of the Stem Cell Engineering Group, Institute of Reconstructive Neurobiology, University of Bonn, LIFE & BRAIN Center. "It is noteworthy that this assay enabled us to observe even early onset of differentiation in iPS cell cultures."

"Stem cells are of great interest for regenerative medicine and drug discovery efforts, and researchers must be able to effectively characterize the potency status of pluripotent stem cells to advance their progress toward clinical use," said Linda Meeh, Ph.D., Director of Marketing for Immunoassays and Multiplexing.

Meeh continued, "MILLIPLEX® MAP kits offer a quick and simple way to achieve this characterization, generating a comprehensive assessment of the overall quality of cell cultures."

Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,600+ scientific posters on ePosters
  • More than 3,800+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Merck Millipore Announces Incoming CEO and President
Udit Batra, Ph.D. will be responsible for the organization’s strategic direction to drive growth and product innovation as well as oversight of the worldwide operations.
Tuesday, April 15, 2014
Merck Millipore and CCRM Collaborate to Optimize Conditions for Large-scale Stem Cell Cultivation
Collaboration to deliver optimized methodologies for cultivation of stem cells on microcarriers in Mobius® CellReady bioreactor systems.
Tuesday, March 13, 2012
Scientific News
Snapshot Turns T Cell Immunology on its Head
New research may have implications for 1 diabetes sufferers.
Developing a Gel that Mimics Human Breast for Cancer Research
Scientists at the Universities of Manchester and Nottingham have been funded to develop a gel that will match many of the biological structures of human breast tissue, to advance cancer research and reduce animal testing.
Lung Repair and Regeneration Gene Discovered
New role for hedgehog gene offers better understanding of lung disease.
Restoring Vision with Stem Cells
Age-related macular degeneration (AMRD) could be treated by transplanting photoreceptors produced by the directed differentiation of stem cells, thanks to findings published today by Professor Gilbert Bernier of the University of Montreal and its affiliated Maisonneuve-Rosemont Hospital.
The Age of Humans Controlling Microbes
Engineered bacteria could soon be used to detect environmental toxins, treat diseases, and sustainably produce chemicals and fuels.
Gene Expression: A Snapshot of Stem Cell Development
New genes found that regulate development of stem cells.
Tissue-Engineered Colon from Human Cells
A study by scientists at Children’s Hospital Los Angeles has shown that tissue-engineered colon derived from human cells is able to develop the many specialized nerves required for function, mimicking the neuronal population found in native colon.
Tension Helps Heart Cells Develop Normally in the Lab
Stanford engineers have uncovered the important role tension plays in growing heart cells out of the body.
Urine Excretion From Stem Cell-Derived Kidneys
Researchers report a strategy for enabling urine excretion from kidneys grown from stem cells.
Stem Cell Research Hints at Evolution of Human Brain
Researchers at UC San Francisco have succeeded in mapping the genetic signature of a unique group of stem cells in the human brain that seem to generate most of the neurons in our massive cerebral cortex.
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,600+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,800+ scientific videos