Corporate Banner
Satellite Banner
Stem Cells, Cellular Therapy & Biobanking
Scientific Community
Become a Member | Sign in
Home>News>This Article

Synthetic Surface for Expansion of Human Mesenchymal Stem Cells in Xeno-Free, Chemically Defined Culture

Published: Friday, August 16, 2013
Last Updated: Friday, August 16, 2013
Bookmark and Share
This study evaluates the ability of the Corning Synthemax Surface to support long-term expansion of hMSCs in serum-free, defined medium.

Human mesenchymal stem cells (HMSCs) possess three properties of great interest for the development of celltherapies and tissue engineering: multilineage differentiation, immunomodulation, and production of trophic factors. Efficient ex vivo expansion of hMSCs is a challenging requirement for large scale production of clinical grade cells. Low-cost, robust, scalable culture methods using chemically defined materials need to be developed to address this need. This study describes the use of a xeno-free synthetic peptide acrylate surface, the Corning® Synthemax® Surface, for culture of hMSCs in serum-free, defined medium. Cell performance on the Corning Synthemax Surface was compared to cells cultured on biological extracellular matrix (ECM) coatings in xeno-free defined medium and in traditional conditions on tissue culture treated (TCT) plastic in fetal bovine serum (FBS) supplemented medium. Our results show successful maintenance of hMSCs on Corning Synthemax Surface for eight passages, with cell expansion rate comparable to cells cultured on ECM and significantly higher than for cells in TCT/FBS condition. Importantly, on the Corning Synthemax Surface, cells maintained elongated, spindle-like morphology, typical hMSC marker profile and in vitro multilineage differentiation potential. We believe the Corning Synthemax Surface, in combination with defined media, provides a complete synthetic, xeno-free, cell culture system for scalable production of hMSCs.

This article is published online in PLoS One and is free to access.

Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,800+ scientific posters on ePosters
  • More than 4,000+ scientific videos on LabTube
  • 35 community eNewsletters

Sign In

Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Biobanking in a Constantly Developing Medical World
Biomaterial resources such as tissues, cells, blood, and serum have played a critical role in academic research.
Wednesday, November 06, 2013
International Heart Valve Bank Survey: A Review of Processing Practices and Activity Outcomes
Since the first heart valve bank (HVB) started in New Zealand in 1962, the recovery, processing, and storage techniques have been constantly evolving.
Wednesday, November 06, 2013
Developing a Semantically Rich Ontology for the Biobank-Administration Domain
Recent research has provided compelling examples of using semantic web technologies, such as ontologies, to retrieve research-relevant data from biobanks.
Tuesday, October 15, 2013
Patient Understanding and Awareness of their Roles as Research Stakeholders in a Cancer Biobank
A patient’s informed consent should imply that the patient has received complete information and fully understands its meaning.
Tuesday, September 10, 2013
Telethon Network of Genetic Biobanks: a Key Service for Diagnosis and Research on Rare Diseases
Recent advances in the technology of molecular biology and genetics have contributed to raise the awareness of the importance of coordinated biobanking activity.
Tuesday, September 10, 2013
Cryopreservation Causes Genetic and Epigenetic Changes in Zebrafish Genital Ridges
Recent studies have pointed out that cryopreservation can cause molecular alterations in cells at different levels that may interfere with normal embryo development.
Thursday, July 18, 2013
BioBanking as the Central Tool for Translational Medicine
Global healthcare changes forms the basis for the rapid development of centralized large scale biobank facilities.
Monday, June 24, 2013
Establishing a Southern Swedish Malignant Melanoma OMICS and Biobank Clinical Capability
Current research developments and future healthcare solutions are expected to be closely linked to the utility of biobank initiatives.
Friday, March 22, 2013
Effect of Different Freezing Rates During Cryopreservation of Rat MSCs using Combinations of HES and DMSO
In this study researchers from Fraunhofer Institute for Cell Therapy and Immunology attempt an initial comparison of two independent factors: freezing rates and cryopreservation.
Thursday, November 08, 2012
Cryopreserved Reticulocytes Derived from Stem Cells can be Invaded by Cryopreserved Plasmodium vivax Isolates
The cryopreservation method described in this paper provides a continuous and substantial source of reticulocytes, possibly a first step to achieve the continuous in vitro culture of P. vivax.
Wednesday, November 07, 2012
New national Biobank of The Danish Center for Strategic Research on Type 2 Diabetes
This paper aims to describe the biobank logistics and how the biological samples are collected, handled, shipped and stored.
Friday, November 02, 2012
Human Blood RNA Stabilization in Samples Collected and Transported for a Large Biobank
The study strongly suggests that special collection tubes are necessary for RNA stabilization and they should be used for establishing new biobanks.
Friday, October 05, 2012
Biobanking for Health Research in Brazil: Present Challenges and Future Directions
This article outlines and discusses Brazil's new regulations on the use of human biological materials for research.
Monday, September 10, 2012
Evaluation of Distinct Freezing Methods and Cryoprotectants for Human Amniotic Fluid Stem Cells Cryopreservation
Researchers from the University of São Paulo Medical School have evaluated the effects of two cryopreservation methods and four distinct cryoprotectants in the AFSCs viability and main characteristics maintenance after liquid nitrogen storage.
Monday, August 06, 2012
IT Solutions for Privacy Protection in Biobanking
In this article, researchers from the University of Klagenfurt discuss current European public opinion on privacy issues and concerning informed consent, proposing IT solutions that help to harmonize research needs and societal demands.
Saturday, August 04, 2012
Scientific News
Fat Cells Originating from Bone Marrow Found in Humans
Cells could contribute to diabetes, heart disease.
Ancient Viral Molecules Essential for Human Development
Genetic material from ancient viral infections is critical to human development, according to researchers at the Stanford University School of Medicine.
CRI Identifies Emergency Blood-formation Response
Researchers report that when tissue damage occurs, an emergency blood-formation system activates.
New Way to Force Stem Cells to Become Bone Cells
Potential therapies based on this discovery could help people heal bone injuries or set hardware, such as replacement knees and hips.
Dead Bacteria to Kill Colorectal Cancer
Scientists from Nanyang Technological University (NTU Singapore) have successfully used dead bacteria to kill colorectal cancer cells.
Promise of Newborn Stem Cells to Revolutionize Clinical Practice
In this article Shweta Sharma, PhD, discusses the potential of an Umbilical Cord Blood bank as an untapped source of samples for research and clinical trials.
The Life Story of Stem Cells
A model analyses the development of stem cell numbers in the human body.
Novel Stem Cell Line Avoids Risk of Introducing Transplanted Tumors
Progenitor cells might eventually be used to repair or rebuild damaged or destroyed organs.
Advancing Genome Editing of Blood Stem Cells
Genome editing techniques for blood stem cells just got better, thanks to a team of researchers at USC and Sangamo BioSciences.
Molecule Proves Key to Brain Repair After Stroke
Scientists found that a molecule known as growth and differentiation factor 10 (GDF10) plays a key role in repair mechanisms following stroke.
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,800+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,000+ scientific videos