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Gene Expression Patterns in Donor-matched Mesenchymal Stem Cells and Osteocytes from Human Trabecular Bone
Dragos Ilas, Sarah Churchman, Peter Giannoudis, Dennis McGonagle and Elena Jones

The study aimed to investigate gene expression profiles of osteocytes in healthy and OA bone and to compare with donor - matched MSCs.

Pharmacological Responses in Cultured Human iPSC-Derived Cortical Neurons Using Multi-Electrode Array
Aoi Odawara (1,2), Hiroki Katoh (1), Naoki Matsuda (1), Karolina Szczesna (3), Yichen Shi (3), Ryan Arant (4), Hideyasu Jiko (4), Ikuro Suzuki (1)

The functional network of human induced pluripotent stem cell (hiPSC)-derived neurons is a potentially powerful in vitro model for evaluating disease mechanisms and drug responses. However, the culture time required for the full functional maturation of individual neurons and networks is uncertain. We investigated the development of spontaneous electrophysiological activity and pharmacological responses for over 1 year in culture using multi-electrode arrays (MEAs).

Translational Research of oral Neural Crest-Derived Stem Cells (oNCSCs) in Regenerative Dentistry
Grimm W.-D1,2,4, S. N. Alekseenko9, D. V. Bobryshev1, W. Duncan11, B. Giesenhagen3, E. Gubareva9, Sema S. Hakki6, O.V. Pershina7, I. Schau4, S. V. Sirak1, E.G. Skurikhin7, A. A. Sletov1, F. Witte10, G. Varga8, O. V. Vladimirova1, M.A. Vukovic2, D. Widera5

In this review poster, we summarize current knowledge on the oral neural crest-derived stem cell populations (oNCSCs) and discuss their potential in regenerative periodontology as a part of regenerative dentistry.

Different Pathways of Regenerative Medicine
Clemens Heyder, Daniela Weber

Altough regenerative medicine is rapidly developing there is neither a technical definition nor a common use in practice. The aim of this study is to draw the line at the limits of regeneration and medicine in order to figure out a proper understanding of regenerative medicine. Furthermore, the analysis reveals a prospering future. It has shown that regenerative medicine technologies are used for a variety of therapeutic applications.

Design and Validation of Bioluminescent Assays for 3D Cell Culture Models Poster
Terry L. Riss, Michael P. Valley, Chad A. Zimprich, Andrew L. Niles, Kevin R. Kupcho and Dan F. Lazar

Cells cultured in 3D model systems often acquire relatively large in vivo-like structures compared to the thickness of a 2D monolayer of cells grown on standard plastic plates.

iPSC-Derived Cardiomyocytes and Luciferase Reporters: A Robust Reporting Platform for Monitoring Cardioprotection and Pathway Biology in Endogenous Human Tissue Cells
Fiene, S., Thompson, A., Niles, A., Robers, M., Anson, B.

Pathophysiological conditions, medical interventions, and off-target toxicities can all result in cellular oxidative stress. In cardiac myocytes, prolonged and/or excessive oxidative stress can lead to cardiotoxicity: a primary cause of developmental delays, black-box warnings, and post-launch withdrawal of pharmaceuticals.

Whole Transcriptome RNA-SEQ with Ion Torrent Platform: FFPE, Fresh LCM and FFPE LCM Samples. Increasingly Difficult
Sara Franceschi1, Paolo Are*ni1, Marco La Ferla1, Generoso Bevilacqua1,2, Chiara Maria Mazzan*1 , Francesca Lessi1

We developed a high performance method to analyze the whole transcriptome of our FFPE samples, obtaining a very high number of reads (78,186,377 usable reads) perfectly comparable with samples with a large amount of RNA such as samples obtained from cells or fresh tissues.

Human iPSC-derived hepatocytes and cardiomyocytes for drug toxicity testing
AnnandRR; Vardaro R; Hamilton B; Akakira R; Tamura K; Yoshida S; Lin YC; Toyoda D; Kogami H; Okuda Y; Watanabe T; Inamura M

Human iPS-derived hepatocytes (ReproHepato™) and cardiomyocytes (ReproCardio 2™) are useful for in vitro toxicity assays.

Development of a novel xeno-free medium for feeder-free culture of human stem cells
Annand R; Okuda Y; Inamura M

A new xeno-free medium (ReproXFTM) has been developed for feeder-free culture of stem cells.

Novel culture medium using a small-molecule agonist of thrombopoetin receptor
Hondo M1; Nishino T2; Inamura M1

ReproHSC medium for the culture of hematopoietic stem cells. facilitates the culture and expansion of CD34+/CD38- cells that retain their HSC properties

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Showing Results 1 - 10 of 86
Scientific News
A Boost for Regenerative Medicine
Growing tissues and organs in the lab for transplantation into patients could become easier after scientists discovered an effective way to produce three-dimensional networks of blood vessels, vital for tissue survival yet a current stumbling block in regenerative medicine.
Heart Defect Prediction Technology Could Lead to Earlier, More Informed Treatment
Experimental method uses genetics-guided biomechanics, patient-specific stem cells.
Immune Cells Remember Their First Meal
Scientists at the University of Bristol have identified the trigger for immune cells' inflammatory response – a discovery that may pave the way for new treatments for many human diseases.
Cancer Cells Coordinate to Form Roving Clusters
Rice University scientists identify ‘smoking gun’ in metastasis of hybrid cells.
Bio-Mimicry Method For Preparing & Labeling Stem Cells Developed
Method allows researchers to prepare mesenchymal stem cells and monitor them using MRI.
Transcription Factor Isoforms Implicated in Colon Diseases
UC Riverside study explains how distribution of two forms of a transcription factor in the colon influence risk of disease.
New Bio-Glass Could Make it Possible to Re-Grow or Replace Cartilage
Researchers at Imperial College London have developed a material that can mimic cartilage and potentially encourage it to re-grow.
Stem Cell Advance Could Be Key Step Toward Treating Deadly Blood Diseases
UCLA scientists get closer to creating blood stem cells in the lab.
Harnessing Engineered Slippery Surfaces For Tissue Repair
A new method could facilitate the transfer of intact regenerating cell sheets from the culture dish to damaged tissues in patients.
Brazilian Zika Virus Strain Causes Birth Defects in Experimental Models
First direct experimental proof of causal effect, researchers say.
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