Whole Transcriptome RNA-SEQ with Ion Torrent Platform: FFPE, Fresh LCM and FFPE LCM Samples. Increasingly Difficult Sara Franceschi1, Paolo Are*ni1, Marco La Ferla1, Generoso Bevilacqua1,2, Chiara Maria Mazzan*1 , Francesca Lessi1
We developed a high performance method to analyze the whole transcriptome of our FFPE samples, obtaining a very high number of reads (78,186,377 usable reads) perfectly comparable with samples with a large amount of RNA such as samples obtained from cells or fresh tissues.
Human iPSC-derived hepatocytes and cardiomyocytes for drug toxicity testing AnnandRR; Vardaro R; Hamilton B; Akakira R; Tamura K; Yoshida S; Lin YC; Toyoda D; Kogami H; Okuda Y; Watanabe T; Inamura M
Human iPS-derived hepatocytes (ReproHepato™) and cardiomyocytes (ReproCardio 2™) are useful for in vitro toxicity assays.
Development of a novel xeno-free medium for feeder-free culture of human stem cells Annand R; Okuda Y; Inamura M
A new xeno-free medium (ReproXFTM) has been developed for feeder-free culture of stem cells.
Novel culture medium using a small-molecule agonist of thrombopoetin receptor Hondo M1; Nishino T2; Inamura M1
ReproHSC medium for the culture of hematopoietic stem cells. facilitates the culture and expansion of CD34+/CD38- cells that retain their HSC properties
Human iPSC-derived hepatocytes ReproHepateTM for CYP assay and drug toxicity testing Annand R; Akahira R; Tamura K; Inamura M
We developed iPSC-derived hepatocytes and used them for CYP induction and cytotoxicity assays.
Alzheimer's disease model cells derived from human iPS cells Honda M; Kitogo T; Kogami H; Inamura M
Alzheimer's disease model neurons were created from iPS cells to enable functional, cell-based assays for AD.
Continuous Collection of Stem Cells from a Human Placenta Perfusion Co-Culture John J.S. Cadwell & James C. Hardy
The potential for human placenta-derived cells to produce stem cells in a hollow fiber bioreactor co-culture system was investigated.
Targeting Cancer Stem Cell-Related miRNAs for Prostate Cancer Therapy ANSHIKA NIKITA SINGH, MEGHNA BARUAH, NEETI SHARMA
The poster focuses on the pivotal function of miRNAs in tumorigenesis by regulation of self renewal and apoptosis via cancer stem cell signalling pathways with special focus on their regulation of Epithelial to Mesenchymal transition in metastatic prostate cancer.
Non-disruptively Count and Quantify Fluorescent iPS Colonies During Secondary Reprogramming: 7 min per 6-well Plate, Dual-fluorescence Whole Well Imaging Cytometry SC Cribbes, S Brightwell, K Kaji
Nexcelom’s Celigo imaging cytometer has been applied to provide automated, rapid assessment of iPS reprogramming.
Hematopoietic Stem Cell Transplantation: Are We There Yet? Shalmali Pendse, Anuradha Vaidya
This review poster comprises of the overall development of hematopoietic stem cell transplantation (HSCT) as a therapy for variety of immuno compromised diseases since 1950 till date. It starts with the general information about hematopoietic stem cells, its sources as well as the types of HSCT. It also contains the role of mesenchymal stem cells in HSCT along with its present scenario and it ends with the future directions regarding HSCT.