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Whole Transcriptome RNA-SEQ with Ion Torrent Platform: FFPE, Fresh LCM and FFPE LCM Samples. Increasingly Difficult
Sara Franceschi1, Paolo Are*ni1, Marco La Ferla1, Generoso Bevilacqua1,2, Chiara Maria Mazzan*1 , Francesca Lessi1

We developed a high performance method to analyze the whole transcriptome of our FFPE samples, obtaining a very high number of reads (78,186,377 usable reads) perfectly comparable with samples with a large amount of RNA such as samples obtained from cells or fresh tissues.

Human iPSC-derived hepatocytes and cardiomyocytes for drug toxicity testing
AnnandRR; Vardaro R; Hamilton B; Akakira R; Tamura K; Yoshida S; Lin YC; Toyoda D; Kogami H; Okuda Y; Watanabe T; Inamura M

Human iPS-derived hepatocytes (ReproHepato™) and cardiomyocytes (ReproCardio 2™) are useful for in vitro toxicity assays.

Development of a novel xeno-free medium for feeder-free culture of human stem cells
Annand R; Okuda Y; Inamura M

A new xeno-free medium (ReproXFTM) has been developed for feeder-free culture of stem cells.

Novel culture medium using a small-molecule agonist of thrombopoetin receptor
Hondo M1; Nishino T2; Inamura M1

ReproHSC medium for the culture of hematopoietic stem cells. facilitates the culture and expansion of CD34+/CD38- cells that retain their HSC properties

Human iPSC-derived hepatocytes ReproHepateTM for CYP assay and drug toxicity testing
Annand R; Akahira R; Tamura K; Inamura M

We developed iPSC-derived hepatocytes and used them for CYP induction and cytotoxicity assays.

Alzheimer's disease model cells derived from human iPS cells
Honda M; Kitogo T; Kogami H; Inamura M

Alzheimer's disease model neurons were created from iPS cells to enable functional, cell-based assays for AD.

Continuous Collection of Stem Cells from a Human Placenta Perfusion Co-Culture
John J.S. Cadwell & James C. Hardy

The potential for human placenta-derived cells to produce stem cells in a hollow fiber bioreactor co-culture system was investigated.

Targeting Cancer Stem Cell-Related miRNAs for Prostate Cancer Therapy
ANSHIKA NIKITA SINGH, MEGHNA BARUAH, NEETI SHARMA

The poster focuses on the pivotal function of miRNAs in tumorigenesis by regulation of self renewal and apoptosis via cancer stem cell signalling pathways with special focus on their regulation of Epithelial to Mesenchymal transition in metastatic prostate cancer.

Non-disruptively Count and Quantify Fluorescent iPS Colonies During Secondary Reprogramming: 7 min per 6-well Plate, Dual-fluorescence Whole Well Imaging Cytometry
SC Cribbes, S Brightwell, K Kaji

Nexcelom’s Celigo imaging cytometer has been applied to provide automated, rapid assessment of iPS reprogramming.

Hematopoietic Stem Cell Transplantation: Are We There Yet?
Shalmali Pendse, Anuradha Vaidya

This review poster comprises of the overall development of hematopoietic stem cell transplantation (HSCT) as a therapy for variety of immuno compromised diseases since 1950 till date. It starts with the general information about hematopoietic stem cells, its sources as well as the types of HSCT. It also contains the role of mesenchymal stem cells in HSCT along with its present scenario and it ends with the future directions regarding HSCT.

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Scientific News
Growing Spinal Disc Tissue
Scientists develop new method for growing spinal disc tissue in the lab for combating chronic back pain.
Elastic Gel to Heal Wounds
A team of bioengineers at Brigham and Women’s Hospital has developed a new protein-based gel that, when exposed to light, mimics many of the properties of elastic tissue, such as skin and blood vessels.
Regenerative Medicine Biologists Discover a Cellular Structure that Explains Fate of Stem Cells
The findings are presented in the journal Nature.
Engineering Yeast that Speaks
Scientists at the University of Washington say they have engineered yeast cells that can "talk" to one another, using the plant hormone auxin.
NuGEN Scientists Screen 400+ Genes for Fusion Events in Single Assay
Breakthrough proves efficacy of new sample preparation method that could accelerate cancer research and development of treatments and diagnostic tests.
Protein Responsible for Blood Vessel Growth in Tumours Discovered
Scientists have discovered a new protein which triggers the growth of blood vessels in breast cancer tumours which have spread to the brain, a common location which breast cancer can spread to.
Epigenetic Switches that turn Stem Cells into Blood Vessel Cells Uncovered
Researchers at the University of Illinois have identified a molecular mechanism that directs embryonic stem cells to mature into endothelial cells.
Human Trials of Manufactured Blood Within Two Years
The first human trials of lab-produced blood to help create better-matched blood for patients with complex blood conditions has been announced by NHS Blood and Transplant.
Cell that Replenishes Heart Muscle Found by UT Southwestern Researchers
Researchers devise a new cell-tracing technique to detect cells that do replenish themselves.
Global Search for Next Antibiotic
University of Queensland researchers have launched a global search to discover antibiotics capable of combating superbug bacteria that are resistant to current antibiotics.
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