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Effect of Culture Media and Serum Supplementation on the Development of in vitro Fertilized Buffalo Embryos
D Kumar, T Anand, P Palta and M.S. Chauhan

This poster compares the development of buffalo embryos in simple and complex culture media and aims to determine the effects of serum supplementation on the development of buffalo embryos.

High Content Analysis of Neural Stem Cell Expansion and Differentiation
Oksana Sirenko, Allan C. Powe, Steven L. Stice, Karen Cook, Nick Callamaras, Jayne Hesley, Xin Jiang and Evan F. Cromwell

Automated assay methods for monitoring neural stem cell expansion and differentiation using stem cell derived neural cell lines and high content imaging systems have been described.

Neurotoxicity Assays Using iPSC-Derived Neurons and High Content Imaging
Oksana Sirenko, Susan DeLaura, Lucas Chase, Jayne Hesley and Evan F. Cromwell

Neurotoxicity can cause temporary or permanent damage of brain or peripheral nervous system and has been found to be a major cause of neurodegenerative diseases such as Alzheimer’s or Parkinson’s. Accordingly, there is a great interest in developing more predictive, disease relevant cell-based models and efficient screening tools for assessing the neurotoxicity of chemical compounds, drug candidates and environmental agents.

Live Cell Beating Assay Using Human iPSC-derived Cardiomyocytes for Evaluation of Drug Efficacy and Toxicity
Oksana Sirenko, Carole Crittenden, Blake Anson, Jayne Hesley, Yen-Wen Chen, Nick Callamaras and Evan F. Cromwell

A large percentage of new drugs fail in clinical studies due to cardiac toxicity. Development of highly predictive in vitro assays suitable for screening, safety assessment or other environments is therefore extremely important for drug development. Human cardiomyocytes derived from stem cell sources can greatly accelerate the discovery of cardiac drugs and improve drug safety by offering more clinically relevant cell-based models than those presently available.

Regulation of shoot apical meristem development by SEUSS and SEUSS-LIKE 2 in Arabidopsis
Joanne E. Lee and John F. Golz

In Arabidopsis, SEU and SLK2 are redundant components of a regulatory complex that is proposed to promote shoot apical meristem (SAM) formation during embryogenesis. Expression analysis indicates that SEU and SLK2 act upstream of several known SAM regulators, and also regulate auxin accumulation, probably via interaction with auxin response factors.

Expansion of mesenchymal stem cells from frozen UCB
Christophe NP Madsen and Christian Clausen

Umbilical cord blood (UCB) has recently been the focus of clinical applications. UCB contains of hematopoietic stem cells and mesenchymal stem cells (MSC). Clinical studies shows that MSC can be used in regenerative medicine hereby treatment of cardiac diseases. The aim of this project is to establish a protocol for isolation of MSCs from frozen UCB. This study demonstrated that it’s possible to expand MSC.

A high-throughput colony formation assay for profiling novel compounds and RNAi reagents using the Acumen® eX3
Andrew Goulter and Jason Mundin

Cell colony formation assays measure a cell's ability to grow unattached to a surface and have applications in a range of areas including hematopoietic stem cell research, cell transformation studies and the prediction of responses of tumors to chemotherapeutic agents. The results of this study demonstrated that Acumen eX3 can be used as a high-throughput platform for investigation of effects of test compounds and RNAi reagents on cell colony formation.

Gene Expression Profiling of Archived FFPE Samples
Silvia Rüberg, Sabine Classen, Jana Ciomperlik, Dirk Dietrich, Ines Dischinger, Alena Böttcher, Sabrina Schmitz and Bernhard Gerstmayer

According to the BBMRI (Biobanking and Biomolecular Resources Research Infrastructure) about 8,000,000 formalin-fixed paraffin-embedded (FFPE) samples derived from a multitude of different diseases have been collected in medical centers and biobanks all over Europe during the last decades.

New Culture Medium Creates Immune Tolerance Between Two Allogeneic Tissue Cells
Victor Alexander, PhD.; Anthony Passerini, PhD; Emir Hodzic, PhD.

We discovered that in vitro high dose of Progesterone (P) together with FGFa, EGF, VEGF, LPS had dual effect on Liver tissue cells: increased multiplication of Hepatocytes and suppressed all non-parenchymal (NP) liver tissue cells and created immune tolerance between two allogeneic mice Liver tissue

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Showing Results 11 - 20 of 68
Scientific News
Suspect Gene Corrupts Neural Connections
“Diseases of synapses” demo’d in a dish - NIH-funded study.
New Blood: Tracing the Beginnings of Hematopoietic Stem Cells
Researchers uncover earliest clues yet to development of cells that produce all adult blood cells.
Clinical Trial Evaluates Safety of Stem Cell Transplantation in Spine
Phase I clinical trial is recruiting eight patients for the 5-year study.
Dramatic Growth of Grafted Stem Cells in Rat Spinal Cord Injuries
Reprogrammed human neurons extend axons almost entire length of central nervous system.
Stem Cells Show Promise for Stroke in Pilot Study
Findings are published in the journal Stem Cells Translational Medicine.
Researchers Reveal Molecular Competition Drives Adult Stem Cells to Specialize
Competition between Bam and COP9 balances the self-renewal and differentiation functions of ovarian GSCs in fruit flies.
Single-Cell Analysis Holds Promise for Stem Cell and Cancer Research
UCSF researchers use microfluidic technology to probe human brain development.
Pilot Study Shows Stem Cells Hold Promise for Stroke Therapy
A stroke therapy using stem cells extracted from patients' bone marrow has shown promising results in the first trial of its kind in humans.
Molecular Competition Drives Adult Stem Cells to Specialize
Understanding the molecular mechanisms that control the balance between self-renewal and differentiation in adult stem cells is an important foundation for developing therapies to regenerate diseased, injured or aged tissue.
Early Treatment Benefits Infants with Severe Combined Immunodeficiency
NIH-funded study identifies factors contributing to successful stem cell transplants.
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