|Expression of Pluripotency-determining Factors in in vitro Fertilized Buffalo Embryos and Embryonic Stem Cells|
T Anand, D Kumar, M S Chauhan, and P Palta
The POU octamer-binding domain transcription factor Oct-4, Stage-specific embryonic antigens (SSEAs), and Tumor rejection antigens (TRAs), are developmentally regulated during early embryogenesis.
|Effect of Culture Media and Serum Supplementation on the Development of in vitro Fertilized Buffalo Embryos|
D Kumar, T Anand, P Palta and M.S. Chauhan
This poster compares the development of buffalo embryos in simple and complex culture media and aims to determine the effects of serum supplementation on the development of buffalo embryos.
|High Content Analysis of Neural Stem Cell Expansion and Differentiation|
Oksana Sirenko, Allan C. Powe, Steven L. Stice, Karen Cook, Nick Callamaras, Jayne Hesley, Xin Jiang and Evan F. Cromwell
Automated assay methods for monitoring neural stem cell expansion and differentiation using stem cell derived neural cell lines and high content imaging systems have been described.
|Neurotoxicity Assays Using iPSC-Derived Neurons and High Content Imaging|
Oksana Sirenko, Susan DeLaura, Lucas Chase, Jayne Hesley and Evan F. Cromwell
Neurotoxicity can cause temporary or permanent damage of brain or peripheral nervous system and has been found to be a major cause of neurodegenerative diseases such as Alzheimer’s or Parkinson’s. Accordingly, there is a great interest in developing more predictive, disease relevant cell-based models and efficient screening tools for assessing the neurotoxicity of chemical compounds, drug candidates and environmental agents.
|Live Cell Beating Assay Using Human iPSC-derived Cardiomyocytes for Evaluation of Drug Efficacy and Toxicity|
Oksana Sirenko, Carole Crittenden, Blake Anson, Jayne Hesley, Yen-Wen Chen, Nick Callamaras and Evan F. Cromwell
A large percentage of new drugs fail in clinical studies due to cardiac toxicity. Development of highly predictive in vitro assays suitable for screening, safety assessment or other environments is therefore extremely important for drug development. Human cardiomyocytes derived from stem cell sources can greatly accelerate the discovery of cardiac drugs and improve drug safety by offering more clinically relevant cell-based models than those presently available.
|Regulation of shoot apical meristem development by SEUSS and SEUSS-LIKE 2 in Arabidopsis|
Joanne E. Lee and John F. Golz
In Arabidopsis, SEU and SLK2 are redundant components of a regulatory complex that is proposed to promote shoot apical meristem (SAM) formation during embryogenesis. Expression analysis indicates that SEU and SLK2 act upstream of several known SAM regulators, and also regulate auxin accumulation, probably via interaction with auxin response factors.
|Expansion of mesenchymal stem cells from frozen UCB|
Christophe NP Madsen and Christian Clausen
Umbilical cord blood (UCB) has recently been the focus of clinical applications. UCB contains of hematopoietic stem cells and mesenchymal stem cells (MSC). Clinical studies shows that MSC can be used in regenerative medicine hereby treatment of cardiac diseases. The aim of this project is to establish a protocol for isolation of MSCs from frozen UCB. This study demonstrated that it’s possible to expand MSC.
|A high-throughput colony formation assay for profiling novel compounds and RNAi reagents using the Acumen® eX3|
Andrew Goulter and Jason Mundin
Cell colony formation assays measure a cell's ability to grow unattached to a surface and have applications in a range of areas including hematopoietic stem cell research, cell transformation studies and the prediction of responses of tumors to chemotherapeutic agents. The results of this study demonstrated that Acumen eX3 can be used as a high-throughput platform for investigation of effects of test compounds and RNAi reagents on cell colony formation.
|Gene Expression Profiling of Archived FFPE Samples|
Silvia Rüberg, Sabine Classen, Jana Ciomperlik, Dirk Dietrich, Ines Dischinger, Alena Böttcher, Sabrina Schmitz and Bernhard Gerstmayer
According to the BBMRI (Biobanking and Biomolecular Resources Research Infrastructure) about 8,000,000 formalin-fixed paraffin-embedded (FFPE) samples derived from a multitude of different diseases have been collected in medical centers and biobanks all over Europe during the last decades.