|High content imaging based mapping of stem cell phenotypes|
Er Liu, Sebastian Vega, Anthony Kulesa, Jared Bushman, Hak-Joon Sung, Mattew Becker, Joachim Kohn, and Prabhas Moghe
Stem cells possess the ability of self-renewal and multi-lineage differentiation. However, conventional biological assays usually take weeks to months to identify the stem cell fates. Here we present a high content imaging based profiling platform that enables identifying stem cell phenotypes at much early time points. This platform could help monitor and even predict the long term fate of stem cells.
|Hepatitis B virus (HBV) and Human immunodeficiency virus (HIV) antibodies detected by peptide microarrays|
ahmed Abd El Wahed1, Ulrike Beutling2, Ronald Frank2, Gerhard Hunsmann1, and Hans-Joachim Fritz3
HBV and HIVenv chips with overlapping oligopeptides encompassing the full amino acid sequences of HBV and HIV polypeptides were produced. In addition, a chip displaying a library of random 4608 different 15-mers peptides (4608-RPL) was prepared. Both chips were used for analyzing monoclonal antibodies and sera from HIV- and HBV-infected individuals. 4608-RPL could be used for identifying target sequences of antibodies without prior knowledge of the corresponding immunizing antigen.
|SuperNatural: A Database of Available Natural Compounds|
Melanie Füllbeck, Mathias Dunkel and Robert Preissner
The majority of marketed drugs are natural compounds or derivatives thereof. The compounds availability is often unclear. Therefore we have compiled a database of ~50,000 natural compounds. Starting point for in silico screenings are about 2,500 well-known, classified natural compounds or imported molecules. Possible medical applications can be detected and about three million conformers computed to account for the flexibility during usage of the 3D-superposition algorithm.
|Nucleic Acid Reagents and Experimental Results in the NCBI Probe Database|
Svetlana Iazvovskaia, Ilene Karsch Mizrachi, Kirill Rotmistrovsky, and Savani Tatake
Five years ago, the NCBI Probe database (ProbeDB) was established to provide a centralized archive of molecular probes used in biomedical applications. Currently ProbeDB contains around 10 million probes of 65 types including gene silencing agents, in situ hybridization probes, and probes for variation analysis and genome mapping. Presently, ProbeDB is the largest and most extensive database of this type available in public domain.
|Regulatory requirements in the development of advanced therapy products (cell-, gene therapy, tissue engineering products)|
Regulatory authorities are active to ensure a good regulatory environment for somatic cell therapy-, gene therapy- and tissue engineering products (in the EU known as ‘Advanced Therapy Medicinal Products’). Both the EMEA and the FDA have, amongst others, issued guidelines to address the specific aspects of these products
|EFFICIENT LARGE SCALE EXPANSION OF HUMAN EMBRYONIC STEM CELLS: COMPARISON OF 4 DIFFERENT SOLUTIONS FOR SINGLE CELL PASSAGING|
Human embryonic stem cells (hESC) are promising for tissue engineering (TE) purposes due to their unique properties. However, the current standard mechanical passaging technique is time consuming and labour intensive, thus limiting the rate of hESC expansion and possible TE experiments.
|Immunomodulatory Properties of Adipose-Derived Stem Cells Cultured in Autologous Serum|
Eivazova G, Berzins U, Zvaigzne A, Skrastina D, Bogdanova A, Kozlovska T, Lejnieks A
This study investigated whether adipose-derived stem cells (ADSC), cultivated in the medium containing autologous serum, maintain immunomodulatory properties of MSC. Obtained cells were CD73, CD90 and CD105 positive but CD34 and CD45 negative. ADSC underwent in vitro differentiation into adipocytes, osteoblasts and chondroblasts. Immunomodulatory properties of ADSC were investigated by blast transformation reaction and significant suppression of T lymphocyte proliferation was induced in a dose d
|Development of cell culture system on the basis of adult stem cells for modeling of HBV infection|
Zajakina A, Berzins U, Bogdanova A, Kozlovska T
Efforts to elucidate the mechanisms by which HBV infects hepatocytes and causes liver damage have been hampered by the strict virus host range and a lack of suitable cell culture and animal models of HBV infection/replication. The present study has established and elucidated a novel HBV infection/replication in vitro model system based on adipose-derived stem cells differentiated into human hepatocyte-like cell line.
|FOXP3 Gene Expression in Multiple Sclerosis patients before and after Mesenchymal Stem Cell therapy|
Maryam Mohajeri, Mandana Mohyeddin Bonab, Behrooz Nikbin, Ali Farazmand
Multiple Sclerosis (MS) is a chronic inflammatory demyelinating disorder of the CNS. No successful treatment for MS, but one therapeutic strategy in research is the use of bone marrow-derived mesenchymal stem cells (MSC). We studied a group of MS patients who underwented MSCs, assayed for expression of a transcription factor, FOXP3, as a specific marker of MS amelioration in peripheral blood. qRT-PCR on PBMCs showed higher FoxP3 levels.