|Immunomodulatory Properties of Adipose-Derived Stem Cells Cultured in Autologous Serum|
Eivazova G, Berzins U, Zvaigzne A, Skrastina D, Bogdanova A, Kozlovska T, Lejnieks A
This study investigated whether adipose-derived stem cells (ADSC), cultivated in the medium containing autologous serum, maintain immunomodulatory properties of MSC. Obtained cells were CD73, CD90 and CD105 positive but CD34 and CD45 negative. ADSC underwent in vitro differentiation into adipocytes, osteoblasts and chondroblasts. Immunomodulatory properties of ADSC were investigated by blast transformation reaction and significant suppression of T lymphocyte proliferation was induced in a dose d
|Development of cell culture system on the basis of adult stem cells for modeling of HBV infection|
Zajakina A, Berzins U, Bogdanova A, Kozlovska T
Efforts to elucidate the mechanisms by which HBV infects hepatocytes and causes liver damage have been hampered by the strict virus host range and a lack of suitable cell culture and animal models of HBV infection/replication. The present study has established and elucidated a novel HBV infection/replication in vitro model system based on adipose-derived stem cells differentiated into human hepatocyte-like cell line.
|FOXP3 Gene Expression in Multiple Sclerosis patients before and after Mesenchymal Stem Cell therapy|
Maryam Mohajeri, Mandana Mohyeddin Bonab, Behrooz Nikbin, Ali Farazmand
Multiple Sclerosis (MS) is a chronic inflammatory demyelinating disorder of the CNS. No successful treatment for MS, but one therapeutic strategy in research is the use of bone marrow-derived mesenchymal stem cells (MSC). We studied a group of MS patients who underwented MSCs, assayed for expression of a transcription factor, FOXP3, as a specific marker of MS amelioration in peripheral blood. qRT-PCR on PBMCs showed higher FoxP3 levels.
|Three-Dimensional Cell Culture Within A Biomimetic Hydrogel Based On Chitosan And Hyaluronan|
A growing demand for study of cells within biologically realistic, in vitro, microenvironments requires a paradigm shift in cell culture technology: from cell culture on 2-D, rigid substrata to cell growth within a 3-D space, defined by malleable materials composed of biologically “smart” compounds.1, 2
Therapeutic issues addressed by tissue engineering and regenerative medicine require similar materials, customized for human application in a wide variety of treatment circumstances: ranging
|Characterization of enamel and dentin protein expression in bioengineered human tooth tissues|
Sheila Soroushian1, Monica Duailibi2, Silvio Duailibi2, Weibo Zhang1, Robert Litterman1, Ivy Pruit1, Pamela C. Yelick1
Here we have used previously described methods to bioengineer teeth from human dental stem cells (hDSC). We we were able to demonstrate that dissociated hDSC-seeded polymer constructs can form bioengineered teeth that are morphologically similar to natural human teeth at both gross and microscopic levels. We anticipate that further modifications of this approach will eventually result in reliable methods to bioengineer replacement teeth in humans.
|The advancement of feeder-independent culture systems for undifferentiated human pluripotent stem cells towards greater regulatory compliance|
Defined and feeder-independent cell culture systems provide a platform for greater reproducibility and standardization in human pluripotent stem cell (hPSC) research, mTeSR 1 has become the most widely cited feeder independent system for the culture of undifferentiated human ES and iPSC.
|Omentum-derived stromal cells improve myocardial regeneration|
Luigi Balducci1, Rocco De Siena1, Antonella Blasi1, Manuela Gessica Montanaro1, Marilisa Saldarelli1, Vittorio Saponaro2, Carmela Martino1, Gaetano Logrieco3,
Human ADSCs (Adipose derived stem cells) could represent an interesting therapy to apply in heart infarction and, more in general, in regenerative medicine. Furthermore, these cells have at least three advantages: good amounts of starting material, expansion feasibility and no ethical restrictions.
|Homing of bone marrow derived mononuclears after intracoronary transventricular transplantation|
Homing of bone marrow derived mononuclears after intracoronary transventricular transplantation
A new method of administration of bone marrow derived mononuclear cells (BMMCs) was studied using the model of acute myocardial infarction in rats. The transplanted cells were detected only in the scar tissue and had fibroblast-like phenotype. They differentiated neither into cardiomyocytes nor into the cells of blood vessels.
|Clinical Evaluation of human embryonic stem cells (hESCs) induced with directed differentiation to gonadotrope cells to cure vasculogenic impotency and to improve coital frequency in males. An open st|
Timothy S. Andersson, David K. Chin, Wendy Hiu Wai Wong
This work demonstrates the clinical efficacy, tolerability and safety of patient-syngenic hESC induced with directed differentiation to gonadotrope cells. Hypothalamus transmits gonadotropin releasing factor to pituitary that sets off LH and FSH to Sertoli cell and Seminiferous tubule resulting Leydig cell to produces testosterone. This potential offers a rationale to evaluate hESCs to cure patients with vasculogenic impotency and to improve coital frequency in males.