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Three-Dimensional Cell Culture Within A Biomimetic Hydrogel Based On Chitosan And Hyaluronan

A growing demand for study of cells within biologically realistic, in vitro, microenvironments requires a paradigm shift in cell culture technology: from cell culture on 2-D, rigid substrata to cell growth within a 3-D space, defined by malleable materials composed of biologically “smart” compounds.1, 2 Therapeutic issues addressed by tissue engineering and regenerative medicine require similar materials, customized for human application in a wide variety of treatment circumstances: ranging

Characterization of enamel and dentin protein expression in bioengineered human tooth tissues
Sheila Soroushian1, Monica Duailibi2, Silvio Duailibi2, Weibo Zhang1, Robert Litterman1, Ivy Pruit1, Pamela C. Yelick1

Here we have used previously described methods to bioengineer teeth from human dental stem cells (hDSC). We we were able to demonstrate that dissociated hDSC-seeded polymer constructs can form bioengineered teeth that are morphologically similar to natural human teeth at both gross and microscopic levels. We anticipate that further modifications of this approach will eventually result in reliable methods to bioengineer replacement teeth in humans.

The advancement of feeder-independent culture systems for undifferentiated human pluripotent stem cells towards greater regulatory compliance

Defined and feeder-independent cell culture systems provide a platform for greater reproducibility and standardization in human pluripotent stem cell (hPSC) research, mTeSR 1 has become the most widely cited feeder independent system for the culture of undifferentiated human ES and iPSC.

Omentum-derived stromal cells improve myocardial regeneration
Luigi Balducci1, Rocco De Siena1, Antonella Blasi1, Manuela Gessica Montanaro1, Marilisa Saldarelli1, Vittorio Saponaro2, Carmela Martino1, Gaetano Logrieco3,

Human ADSCs (Adipose derived stem cells) could represent an interesting therapy to apply in heart infarction and, more in general, in regenerative medicine. Furthermore, these cells have at least three advantages: good amounts of starting material, expansion feasibility and no ethical restrictions.

Homing of bone marrow derived mononuclears after intracoronary transventricular transplantation
Homing of bone marrow derived mononuclears after intracoronary transventricular transplantation

A new method of administration of bone marrow derived mononuclear cells (BMMCs) was studied using the model of acute myocardial infarction in rats. The transplanted cells were detected only in the scar tissue and had fibroblast-like phenotype. They differentiated neither into cardiomyocytes nor into the cells of blood vessels.

Clinical Evaluation of human embryonic stem cells (hESCs) induced with directed differentiation to gonadotrope cells to cure vasculogenic impotency and to improve coital frequency in males. An open st
Timothy S. Andersson, David K. Chin, Wendy Hiu Wai Wong

This work demonstrates the clinical efficacy, tolerability and safety of patient-syngenic hESC induced with directed differentiation to gonadotrope cells. Hypothalamus transmits gonadotropin releasing factor to pituitary that sets off LH and FSH to Sertoli cell and Seminiferous tubule resulting Leydig cell to produces testosterone. This potential offers a rationale to evaluate hESCs to cure patients with vasculogenic impotency and to improve coital frequency in males.

Clinical Evaluation of induced pluripotent stem cells (iPSc) to cure patients with Psoriasis Vulgaris. An Open Study.
Timothy S. Andersson, David K. Chin, Wendy H. Wong

To evaluate the clinical efficacy of iPSc directed to interfere T-cell activation to cure patients with psoriasis. Twenty subjects were enrolled. Each patient was administered iv with syngenic iPS. Those showing lower PASI score were considered cured. The study states that induced iPSCs are safe and impart significant clinical efficacy to cure patients with psoriasis.

Cell Therapy Product Manufacturing in a Hospital Setting
Richard C. Meagher, PhD1,2, Leo I. Gordon, MD1,2, Morris Kletzel, MD1,2,3, Seema Singhal, MD,1,2, Thomas Shook, MT (ASCP)2, Marcelo Villa MT (ASCP)2, Mehboob Merchant SLS (ASCP)2, Brian Stepien2, Jayesh Mehta, MD1,2

This presentation describes in detail the development and establishment of a current good manufacturing practices (cGMP) facility for the purpose of manufacturing cell and tissue products intended for human use in a hospital setting.

PRESENTATION OF A NEW SOURCE FOR TEETH TISSUE ENGINEERING
Fahimeh S. TABATABAEI, Jafar AI, Tahereh S .JAFARZADEH KASHI

Tooth regeneration is a biological technique to solving problems of tooth loss. The detection of various stem cells in unerupted tooth buds, dental pulp or bone marrow has provided opportunities for their management in dentin-pulp repair. However, these cells types are limited by: availability, invasiveness of extraction and in some cases limited proliferative capacity. Herein we hypothesized that endometrial adult stem cells could be induced into odontoblasts.

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Scientific News
Promising Stem Cell Therapy
Animal model of breast-to-brain cancer spread allows testing of therapeutic-cell approach.
A New Lease on Life for Prostate Tissue
Rutgers Cancer Institute of New Jersey research results in development of organoid prostate models
Drugs that Activate Brain Stem Cells May Reverse Multiple Sclerosis
NIH-funded study identifies over-the-counter compounds that may replace damaged cells.
New Transitional Stem Cells Discovered
New stem cells are easier to manipulate, could help future research on reproductive problems.
A New Tool for Understanding ALS: Patients’ Brain Cells
Researchers create a free public library of versatile stem cells from ALS patients.
Limber Lungs: One Type of Airway Cell Can Regenerate Another Lung Cell Type
Findings from animal study have implications for disorders such as chronic obstructive pulmonary disease.
Amniotic Stem Cells Demonstrate Healing Potential
Rice University, Texas Children’s Hospital study proves cells promote vasculature in hydrogel therapy.
Characterizing the Micro-Mechanical Properties of Soft Materials, Tissues and Cells
Ernst Breel of Optics11 discusses the current problems with characterizing the mechanical properties of soft materials, focusing on how the company's Piuma Nanoindenter can assist researchers.
Novel Mechanism Controlling Lung Cancer Stem Cell Growth
Moffitt Cancer Center researchers have discovered a novel mechanism that plays an important role in the maintenance of lung cancer stem cells.
The Switch That Might Tame The Most Aggressive Of Breast Cancers
Garvan researchers have found that so-called ‘triple-negative breast cancers’ are two distinct diseases that likely originate from different cell types. They have also found a gene that drives the aggressive disease, and hope to find a way to ‘switch it off’.
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