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Friday, December 19, 2014
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Induced Pluripotency with Endogenous and Inducible Genes

The recent discovery that two partly overlapping sets of four genes induce nuclear reprogramming of mouse and human has opened up new possibilities for cell replacement therapies. Although the combination of genes that induce pluripotency to some extenet, Oct4 and Sox2 appear to be a prerequisite. The introduction of four genes, several of which have been linked with cancer, using retroviral approaches is however unlikely to be suitable for future clinical applications.

Human Fetal Bone Cells Seeded in Hyaluronic Acid Gel As A Delivery System For Bone Engineering
D.Tenorio, L.A.Applegate, S. Jaccoud, C.Scaletta, D.P. Pioletti

Human fetal bone cells seeded in a hyaluronic acid gel as a delivery system, can survive and proliferate within this gel, are positive for alkaline phosphatase and von Kossa staining, demonstrating the potential of these cells bone engineering.

Development of a Bone Marrow Targeting Cell Therapy
Thomas Kean*, Lori Duesler*, Tambet Teesalu**, Erkki Ruoslahti**, James E Dennis*

One major obstacle in stem cell therapy is poor cell engraftment. To address this issue, concurrent investigations were made to develop novel peptides targeting bone marrow and a method to transiently ‘paint’ targeting molecules onto cells. Painting was studied using a model palmitated peptide. Novel bone marrow targeting peptides were sought using in vivo phage display in a locally irradiated mouse model that has an internal control (untreated leg).

Adaptive control of glycerol & methanol feeding in recombinant Pichia pastoris cultures: Impact on antibody titre
Filipe Ataíde, Ana Raquel Ferreira, João J. Clemente, António E. Cunha, Rui Oliveira

Pichia pastoris processes for heterolougous protein expression are typically run in three phases: a batch phase, an exponential feeding fed-batch phase and a long oxygen transfer limitation (OTL) phase. The final protein titre is often limited by the oxygen availability in the OTL phase. Here a direct adaptive controller is designed for the OTL phase. The controller was validated in pilot P. pastoris cultivations expressing a single chain antibody fragment (ScFv). This work shows that the propos

Critical factors for successful RNAi experiments in primary cells and hard to transfect cell lines
Markus Zumbansen1, Nicole Spottke1, Sheila Offizier1, Allison St. Amand2, Devin Leake2, Ludger Altrogge1, Meike Weigel1, Sandra Domzalski1, Dietmar Lenz, and Herbert Müller-Hartmann1

The amaxa Nucleofector® Technology is a well established method for effective, non-viral transfection of any nucleic acid substrate into hard-to-transfect cells, especially suspension and primary cells. With the Nucleofector® 96-well Shuttle® system high throughput applications such as siRNA-library screenings have become amenable for the first time in these cell types. This renders target validation and identification possible in cell types highly relevant for biomedical research. Here we dis

High Content Image Analysis using a Laser Scanning Microplate Cytometer
Paul Wylie, Ben Schenker, Sarah Payne and Andrew Goulter

Microscope-based high-content instruments offer high optical resolution, however, the limited field of view afforded by their objective lenses can mean lengthy read times for some assays, especially where multiple image capture per well is required. Wherever possible, only a small percentage of the total number of cells present in a test well are analysed to keep plate read times at a minimum, which may not always be ideal. Microplate laser- scanning cytometers, such as TTP LabTech’s Acumen® eX3

Stem Cell Derived Human Neurons in CNS Drug Discovery
L Jackson, C Verastegui, C Wells, D Pau, A Nunn, A Finucane, M Lynch, S Boldt, L Gerrard, S.J. MacKenzie

Central nervous system disorders affect many people worldwide. To increase the quality and number of drugs available for treatments of CNS disorders such as Alzheimer’s, schizophrenia and anxiety, the success rate of CNS drug discovery programmes must be improved. The challenge in drug discovery is to develop more relevant assay systems to identify lead compounds for further development. Cell based screening assays currently used for CNS drug development involve primary cells or commercially

Current status and prospects of the commercial storage of adult stem
Alexander E. Rodin, Anatoly N. Gromyko, Alexander B. Smolyaninov

The authors present a result of survey among 300 residents of the Russian Far East on their potential willingness to store cord blood stem cells of their newborns and/or their own gametes for the future needs. The current status of the cord blood banking system in Russia is also described.

Modular Glass Chip System for the Acquisition of the Electric Activity and Physiological Parameters of Differentiated Stem Cells
P. J. Koester, J. Sakowski, S. M. Buehler, C. Tautorat, H. Altrichter, W. Baumann and J. Gimsa

The EU chemical policy REACH, effective on June 2007, regulates the registration, evaluation and authorization of chemicals. Investigations on the effects of neurotoxic and developmental-neurotoxic substances are required by European and US-American test guidelines. However, at the moment these tests are based on animal experiments contradicting society claims to abolish animal experiments and to implement the 3R-principle. The resulting problem is… How to pair REACH and the 3Rs?

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Showing Results 51 - 60 of 71
Scientific News
Stem Cells Faulty In Duchenne Muscular Dystrophy
In a mouse model of Duchenne muscular dystrophy, muscle stem cells express connective-tissue genes associated with fibrosis and muscle weakness, according to a new study.
Cutting Out the Cellular Middleman
New technology directly reprograms skin fibroblasts for a new role.
Proteins Drive Cancer Cells To Change States
When RNA-binding proteins are turned on, cancer cells get locked in a proliferative state.
Patient’s Own Stem Cells Could Clear a Cloudy Cornea
Researchers use patient’s own cells from the uninjured eye for this process.
Stem Cells from Adult Nose Tissue Used to Cure Parkinson’s Disease in Rats
Researchers produce mature neurons in the laboratory using inferior turbinate stem cells.
Protein Key To Harnessing Regenerative Power Of Blood Stem Cells Unlocked
Stem cells lacking the protein have an increased ability to replicate in the natural environment and following transplantation.
Genetic Errors Linked To Aging Underlie Leukemia That Develops After Cancer Treatment
New research by Daniel Link, MD, and colleagues at The Genome Institute at Washington University has revealed that mutations that accumulate randomly as a person ages can play a role in a fatal form of leukemia that develops after treatment for another cancer.
Stem Cells Can Be Activated To Help Immune System
UCLA researchers have discovered that the Scalloped and Yorkie genes play a key role in how progenitor stem cells are activated to fight infection.
Role of Cancer Stem Cells in Chemo-Resistance
'Wound response' of cancer stem cells may explain chemo-resistance in bladder cancer.
Discovering A New Mechanism For Sex Control In Zebrafish
Research shows that reduction of germ cells yields more males.
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