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EFFICIENT LARGE SCALE EXPANSION OF HUMAN EMBRYONIC STEM CELLS: COMPARISON OF 4 DIFFERENT SOLUTIONS FOR SINGLE CELL PASSAGING

Human embryonic stem cells (hESC) are promising for tissue engineering (TE) purposes due to their unique properties. However, the current standard mechanical passaging technique is time consuming and labour intensive, thus limiting the rate of hESC expansion and possible TE experiments.

Immunomodulatory Properties of Adipose-Derived Stem Cells Cultured in Autologous Serum
Eivazova G, Berzins U, Zvaigzne A, Skrastina D, Bogdanova A, Kozlovska T, Lejnieks A

This study investigated whether adipose-derived stem cells (ADSC), cultivated in the medium containing autologous serum, maintain immunomodulatory properties of MSC. Obtained cells were CD73, CD90 and CD105 positive but CD34 and CD45 negative. ADSC underwent in vitro differentiation into adipocytes, osteoblasts and chondroblasts. Immunomodulatory properties of ADSC were investigated by blast transformation reaction and significant suppression of T lymphocyte proliferation was induced in a dose d

Development of cell culture system on the basis of adult stem cells for modeling of HBV infection
Zajakina A, Berzins U, Bogdanova A, Kozlovska T

Efforts to elucidate the mechanisms by which HBV infects hepatocytes and causes liver damage have been hampered by the strict virus host range and a lack of suitable cell culture and animal models of HBV infection/replication. The present study has established and elucidated a novel HBV infection/replication in vitro model system based on adipose-derived stem cells differentiated into human hepatocyte-like cell line.

FOXP3 Gene Expression in Multiple Sclerosis patients before and after Mesenchymal Stem Cell therapy
Maryam Mohajeri, Mandana Mohyeddin Bonab, Behrooz Nikbin, Ali Farazmand

Multiple Sclerosis (MS) is a chronic inflammatory demyelinating disorder of the CNS. No successful treatment for MS, but one therapeutic strategy in research is the use of bone marrow-derived mesenchymal stem cells (MSC). We studied a group of MS patients who underwented MSCs, assayed for expression of a transcription factor, FOXP3, as a specific marker of MS amelioration in peripheral blood. qRT-PCR on PBMCs showed higher FoxP3 levels.

Three-Dimensional Cell Culture Within A Biomimetic Hydrogel Based On Chitosan And Hyaluronan

A growing demand for study of cells within biologically realistic, in vitro, microenvironments requires a paradigm shift in cell culture technology: from cell culture on 2-D, rigid substrata to cell growth within a 3-D space, defined by malleable materials composed of biologically “smart” compounds.1, 2 Therapeutic issues addressed by tissue engineering and regenerative medicine require similar materials, customized for human application in a wide variety of treatment circumstances: ranging

Characterization of enamel and dentin protein expression in bioengineered human tooth tissues
Sheila Soroushian1, Monica Duailibi2, Silvio Duailibi2, Weibo Zhang1, Robert Litterman1, Ivy Pruit1, Pamela C. Yelick1

Here we have used previously described methods to bioengineer teeth from human dental stem cells (hDSC). We we were able to demonstrate that dissociated hDSC-seeded polymer constructs can form bioengineered teeth that are morphologically similar to natural human teeth at both gross and microscopic levels. We anticipate that further modifications of this approach will eventually result in reliable methods to bioengineer replacement teeth in humans.

The advancement of feeder-independent culture systems for undifferentiated human pluripotent stem cells towards greater regulatory compliance

Defined and feeder-independent cell culture systems provide a platform for greater reproducibility and standardization in human pluripotent stem cell (hPSC) research, mTeSR 1 has become the most widely cited feeder independent system for the culture of undifferentiated human ES and iPSC.

Omentum-derived stromal cells improve myocardial regeneration
Luigi Balducci1, Rocco De Siena1, Antonella Blasi1, Manuela Gessica Montanaro1, Marilisa Saldarelli1, Vittorio Saponaro2, Carmela Martino1, Gaetano Logrieco3,

Human ADSCs (Adipose derived stem cells) could represent an interesting therapy to apply in heart infarction and, more in general, in regenerative medicine. Furthermore, these cells have at least three advantages: good amounts of starting material, expansion feasibility and no ethical restrictions.

Homing of bone marrow derived mononuclears after intracoronary transventricular transplantation
Homing of bone marrow derived mononuclears after intracoronary transventricular transplantation

A new method of administration of bone marrow derived mononuclear cells (BMMCs) was studied using the model of acute myocardial infarction in rats. The transplanted cells were detected only in the scar tissue and had fibroblast-like phenotype. They differentiated neither into cardiomyocytes nor into the cells of blood vessels.

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Scientific News
Manufactured Stem Cells To Advance Clinical Research
Clinical-grade cell line will enable development of new therapies and accelerate early-stage clinical research.
Manufactured Stem Cells to Advance Clinical Research
Clinical-grade cell line will enable development of new therapies and accelerate early-stage clinical research.
Starving Stem Cells May Enable Scientists To Build Better Blood Vessels
Researchers from the University of Illinois at Chicago College of Medicine have uncovered how changes in metabolism of human embryonic stem cells help coax them to mature into specific cell types — and may improve their function in engineered organs or tissues.
Long-Term Culturing of Adult Stem Cells
A new procedure developed by Harvard Stem Cell Institute researchers (HSCI) at Massachusetts General Hospital (MGH) may revolutionize the culturing of adult stem cells.
Naked Mole Rat Exhibits “Extraordinary” Cancer Resistance
Scientists are getting closer to understanding the anti-cancer mechanism of the naked mole rat by making induced pluripotent stem cells.
Solutions for Biotherapeutic Characterization
Innovation to speed the routine.
Reclaiming The Immune System's Assault On Tumors
EPFL study shows a way to reclaim corrupted immune cells.
What Makes a Good Scientist?
It’s the journey, not just the destination that counts as a scientist when conducting research.
Body’s Own Gene Editing System Generates Leukemia Stem Cells
Inhibiting the editing enzyme may provide a new therapeutic approach for blood cancers.
Cirrhosis-Causing Cells Converted to Healthy Liver Cells in Mice
New approach that repairs liver from within may be more efficient than cell transplants.
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