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Correlation between the stem cells differentiation and topography of Zinc oxide nanorods
Newsha Kavosh, James Doonan, Joe Briscoe, Farahnaz Ansari, and Jeremy J. Ramsden

Since the conformation and symmetry may be equally important, we examine the order of nanorods on the surface to simulate the in vitro differentiation of stem cells and we revealed that stem cells respond differently to nanoscale features under the same conditions. Altering the orientation of nanotubular zinc oxide on the surface revealed changes in stem cell behaviour.

Mechanisms of beneficial effects of human placenta MSC transplantation in rats with experimental ischemic stroke
Yarygin K.N., Kholodenko I.V., Konieva A.A., Burunova V.V., Tairova R.T., Gubsky L.V., Cheglakov I.B., PirogovYu.A., Yarygin V.N., Skvortsova V.I.

Human MSC transplantation significantly reduced the volume of experimental ischemic lesion in rat model. It also greatly stimulated stem and progenitor cell proliferation in the subventricular region and hippocampus and cell migration from these areas towards the ischemia site. Beneficial effects of MSC in rats with brain ischemia are likely to be associated with enhancement of host neural stem and progenitor cell proliferation and migration towards the affected area.

Superporous SIKVAV-modified PHEMA scaffold promotes stem cell adhesion
V.Vanecek, S.Kubinova, D.Horak, V.Proks, E.Sykova

Poly(2-hydroxyethyl methacrylate) (PHEMA) is successfully used as a scaffold material for tissue engineering; however, it lacks functional groups that support cell adhesion. The laminin-derived peptide sequence SIKVAV is known to mimic the function of laminin, which is one of the biologically active proteins of the extracellular matrix. In this report, the adhesion of mesenchymal stem cells (MSC) to SIKVAV-modified PHEMA was investigated.

The effect of different magnetic nanoparticle coatings on the efficiency of stem cell labeling
M. Kapcalova, M. Babic, P. Jendelova, V. Herynek, K. Likavcanova, D. Horak, M. Hajek, E. Sykova

Study of volumetric oxygen transfer coefficient in a Taylor vortex flow bioreactor
Patricia Ap. Santiago; Claudio A Suazo; Roberto C Giordano.

In biochemical processes involving cultivation of animal cells, the supply of oxygen in culture medium is an extremely important parameter in the project of bioreactors and its operation. In this work, the global volumetric transfer coefficient of oxygen (KLa) is evaluated in a Taylor vortex flow bioreactor (TVFB) and a correlation of mass transfer, of this equipment, is proposed.

Reliable and Predictive In Vitro Assays for Myelotoxicity and Cardiotoxicity of Kinase Inhibitors
Clarke E (1), Schwengberg S (2), Kettenhofen R (2), Dos Santos G (1), Bohlen H (2)

Kinase inhibitors (KIs) represent a new class of rationally designed drugs. The success of Imatinib has prompted the development of other KIs for the treatment of various cancers and inflammation. Although more successful than conventional therapies, myelotoxicity and cardiotoxicity are often major side effects of KIs. In order to predict if newly developed molecules demonstrated significant myelotoxicity or cardiotoxicity, we assessed a number of TKIs using in vitro models.

Intra-operative Bone Marrow Processing for Bone Regeneration
E. Nedelcu MD, A. Jamali MD, L. Hill RN, J. Nolta PhD, D. Vierria RN, M. Hoze RN, D. Taylor MD PhD, C. Gresens MD, D. Dwyre MD

Concentrated stem cell products obtained from autologous bone marrow aspirate were previously shown to regenerate avascular necrosis of the bone and non-union fractures. The aspirate processing was performed using apheresis technology, however, a detailed protocol has not been published yet. The aim of our study was to develop a clinical protocol using the CobeSpectra apheresis device to process the bone marrow aspirate.

The Expression and Activity of MMPs during Zebrafish (Danio rerio) Development
N.D. Harris, D.H. Li, J.Y. Keow, C. Po, A. Hong, K. Herrmann, B.D. Crawford

Recent advances have made the zebrafish an attractive system for studying MMP activity. However, there are few commercially available antibodies against MMPs that are suitable for studies using zebrafish. The objectives of the study presented in the poster were to develop effective immunological reagents for the study of MMPs in zebrafish and to test commercially available narrow-spectrum fluorogenic MMPs substrates in in vivo zymography.

Genetic profile of human embryonic stem cells (hESC) grown under different O2 concentrations
Sonia Prado, David Montaner, Dario Melguizo,Rubén Moreno, Joaquín Dopazo and Miodrag Stojkovic.

The development of mammalian embryos during the early stages takes place in a hypoxic environment. After 11 weeks of pregnancy the mother’s blood begins to flow freely in the placental space increasing the partial pressure of oxygen [1-3]. Therefore, we hypothesized that in vitro growth of hESC in normoxic conditions (21% O2) may change the profile and differentiation potential of hESC.

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Showing Results 51 - 60 of 80
Scientific News
Regenerative Medicine Biologists Discover a Cellular Structure that Explains Fate of Stem Cells
The findings are presented in the journal Nature.
Engineering Yeast that Speaks
Scientists at the University of Washington say they have engineered yeast cells that can "talk" to one another, using the plant hormone auxin.
NuGEN Scientists Screen 400+ Genes for Fusion Events in Single Assay
Breakthrough proves efficacy of new sample preparation method that could accelerate cancer research and development of treatments and diagnostic tests.
Protein Responsible for Blood Vessel Growth in Tumours Discovered
Scientists have discovered a new protein which triggers the growth of blood vessels in breast cancer tumours which have spread to the brain, a common location which breast cancer can spread to.
Epigenetic Switches that turn Stem Cells into Blood Vessel Cells Uncovered
Researchers at the University of Illinois have identified a molecular mechanism that directs embryonic stem cells to mature into endothelial cells.
Human Trials of Manufactured Blood Within Two Years
The first human trials of lab-produced blood to help create better-matched blood for patients with complex blood conditions has been announced by NHS Blood and Transplant.
Cell that Replenishes Heart Muscle Found by UT Southwestern Researchers
Researchers devise a new cell-tracing technique to detect cells that do replenish themselves.
Global Search for Next Antibiotic
University of Queensland researchers have launched a global search to discover antibiotics capable of combating superbug bacteria that are resistant to current antibiotics.
Protein Plays Unexpected Role in Embryonic Stem Cells
A protein long believed to only guard the nucleus also regulates gene expression and stem cell development.
Vital Genes in Fat Production Found
Discovery could help researchers increase ‘good fat’ in patients, reduce ‘bad’.
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