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Saturday, January 31, 2015
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Development of a Lab-on-a-Chip for the Characterization of Human Cells
Richter, L., Stepper, C., Mak, A., Brückl, H. and Ertl, P.

Cell chips are developed to continuously monitor mammalian cell population dynamics in a non-invasive manner. In the presented work we describe the design, fabrication and characterization of a lab-on-a-chip for quantitative cell analysis.

CyTRAK™ Probes: Novel Nuclear and Cytoplasm Discriminators Compatible with GFP-Based HCS and HTS Assays
RJ Errington, LH Patterson, R Edward and PJ Smith

Image-based high-content screening assays, demand solutions for image segmentation and cellular compartment encoding to track critical events – for example those presented by GFPreporters within cell cycle tracking and GPCR translocation assays. We have designed nuclear and cytoplasm discriminator CyTRAK™ probes - spectrally compatible with all variants of GFPreporters offering new solutions in cytometry.

A novel Hydrogel Mountant - CyGEL™ - Enables Temporospatial HCS Imaging Assays of Live non-Adherent Cells
Edward R, Errington RJ, Patterson LH, Ogrodzinski S and Smith PJ

New technologies are needed to deal with live cell-based HCS assays on non-adherent cells or to exploit suspension cells in fluidic systems. Here we describe CyGEL™ - a thermo-reversible hydrogelbased 4-D immobilization technology for live cell location without imposing an anchoring to a substrate.

DNA Methylation Analysis – Reliable Cell Characterization in Regenerative Medicine
Uli Hoffmueller, Stephen Rapko, Udo Baron, Georg Wieczorek, Alexander Hellwag, Cornelia Krüger, Stefan Kärst, Leslie Wolfe and Sven Olek

We demonstrate that DNA methylation patterns can serve as characteristic markers to distinguish different cell types. We have identified panels of methylation markers that are specific to mesenchymal stem cells or various differentiated cell types in the mesenchymal lineage. This method of cell type identification has a number of advantages over conventional markers in that it is robust, is both qualitative and quantitative.

An Emerging Revolution in Automated 3D Cell Culture
Cal Trepagnier, Stacey Szymanski, Kevin Huff, Alision Rush, Richard Peltier, Berta strulovici, Rebecca Lobo and Robin Felder

Market pressure exists for new paradigms to meet the demands for more rapid and reproducible cell production to keep up with advances in cell-based assays used in drug discovery and development. Novel automation platforms enable high productivity on three dimensional surfaces.

The Effect of Microwell size and Inoculation Density on the Automated Processing of Stem Cells
Rosario Scott, Farlan S Veraitch, Spyridon Gerontas, Gary J Lye and Chris Mason

The work presented in this poster covers the investigation of key parameters for the expansion of stem cells in an automated micro-well format. The effect of inoculation seeding density (ICD), well size, passage number and feeding strategies on the proliferation of human mesenchymal stromal cells (hMSC) was investigated. In parallel the effect of well size and seeding density on the proliferation of mouse embryonic stem (mES) cells was also examined.

Identification of Chemokines and Cytokines Involved in the Migration of MSC to Bone Marrow as Well as to Damaged Tissues
Tondreau T, Meuleman N, Massy M, Delforge A, Bron D and Lagneaux L.

Recent in vivo studies have demonstrated that transplanted MSC in mice were able to migrate into various tissues including lung, brain, bone marrow, heart and many others. Nevertheless, mechanisms responsible for the MSC migration remain unclear.

DNA Microarrays for Microbiological Diagnosis in Stem Cell Cultures
F. Cobo, A. Nieto, P. Catalina, JL. Cortés, A. Barroso, C. Cabrera, R. Montes, A. Barnie and A. Concha

In stem cell cultures there is the possibility of infectious disease transmission to the recipients. Any microbial contamination of the donor`s biological products or introduced during the manufacturing process can potentially present a serious hazard to the recipients. The majority of potential contaminants are mycoplasma and other bacterias, yeasts and fungi. Moreover, viral and prion contamination of cell cultures and “feeder” cells is also a common risk.

In vitro Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells Towards the Osteoblast Lineage. A Model to Study the Effects of Cancer Cells on Bone Formation
Hichame Id Boufker, Fabrice Journé, Laurence Lagneaux, Tatiana Tondreau and Jean-Jacques Body

This poster describes an in vitro model of DAG-induced osteogenic differentiation of bone marrow mesenchymal stem using various osteoblastic markers. Human MSC should constitute a useful in vitro tool to the effects of osteotropic cancer cells on osteoblast differentiation and to better understand the inhibition of normal formation during the process of tumor-induced osteolysis.

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Showing Results 61 - 70 of 71
Scientific News
Novel Organoid Matrix Enables Long-Term Culture of Human Hepatocytes
Organoid growth matrix to enable long-term culture of genome-stable bipotent stem cells from adult human liver.
New Cells May Help Treat Diabetes
UI group creates insulin-producing cells that normalize blood-sugar levels in diabetic mice.
Telomere Extension Turns Back Aging Clock In Cultured Cells
Researchers delivered a modified RNA that encodes a telomere-extending protein to cultured human cells. Cell proliferation capacity was dramatically increased, yielding large numbers of cells for study.
Nanodiamonds Deliver Cancer Drug To Kill Chemoresistant Cancer Stem Cells More Effectively
Delivery of Epirubicin by nanodiamonds resulted in a normally lethal dosage of Epirubicin becoming a safe and effective dosage for treatment of liver cancer.
New Revolutionary Culturing Technique for Liver and Pancreas
Culturing of mini-guts from single mouse intestine stem cells.
Bone Stem Cells Shown To Regenerate Bone And Cartilage In Adult Mice
Cells could be exploited to treat osteoarthritis and osteoporosis.
Novogen Announces Important Discovery in Regenerative Medicine Program
A key proof-of-concept step to develop drugs capable of stimulating the function of brain tissue stem cells.
Researchers Grow Functional Tissue-Engineered Intestine from Human Cells
Regenerative medicine technique brings surgeons one step closer to helping patients.
Defining Stages Of Stem Cell Reprogramming
Method developed by UCLA researchers to define the stages of stem cell reprogramming.
Discovery Links Shift In Metabolism To Stem Cell Renewal
Mouse cells exposed to the metabolite alpha-ketoglutarate became more likely to renew themselves.
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