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Human Fetal Bone Cells Seeded in Hyaluronic Acid Gel As A Delivery System For Bone Engineering
D.Tenorio, L.A.Applegate, S. Jaccoud, C.Scaletta, D.P. Pioletti

Human fetal bone cells seeded in a hyaluronic acid gel as a delivery system, can survive and proliferate within this gel, are positive for alkaline phosphatase and von Kossa staining, demonstrating the potential of these cells bone engineering.

Development of a Bone Marrow Targeting Cell Therapy
Thomas Kean*, Lori Duesler*, Tambet Teesalu**, Erkki Ruoslahti**, James E Dennis*

One major obstacle in stem cell therapy is poor cell engraftment. To address this issue, concurrent investigations were made to develop novel peptides targeting bone marrow and a method to transiently ‘paint’ targeting molecules onto cells. Painting was studied using a model palmitated peptide. Novel bone marrow targeting peptides were sought using in vivo phage display in a locally irradiated mouse model that has an internal control (untreated leg).

Adaptive control of glycerol & methanol feeding in recombinant Pichia pastoris cultures: Impact on antibody titre
Filipe Ataíde, Ana Raquel Ferreira, João J. Clemente, António E. Cunha, Rui Oliveira

Pichia pastoris processes for heterolougous protein expression are typically run in three phases: a batch phase, an exponential feeding fed-batch phase and a long oxygen transfer limitation (OTL) phase. The final protein titre is often limited by the oxygen availability in the OTL phase. Here a direct adaptive controller is designed for the OTL phase. The controller was validated in pilot P. pastoris cultivations expressing a single chain antibody fragment (ScFv). This work shows that the propos

Critical factors for successful RNAi experiments in primary cells and hard to transfect cell lines
Markus Zumbansen1, Nicole Spottke1, Sheila Offizier1, Allison St. Amand2, Devin Leake2, Ludger Altrogge1, Meike Weigel1, Sandra Domzalski1, Dietmar Lenz, and Herbert Müller-Hartmann1

The amaxa Nucleofector® Technology is a well established method for effective, non-viral transfection of any nucleic acid substrate into hard-to-transfect cells, especially suspension and primary cells. With the Nucleofector® 96-well Shuttle® system high throughput applications such as siRNA-library screenings have become amenable for the first time in these cell types. This renders target validation and identification possible in cell types highly relevant for biomedical research. Here we dis

High Content Image Analysis using a Laser Scanning Microplate Cytometer
Paul Wylie, Ben Schenker, Sarah Payne and Andrew Goulter

Microscope-based high-content instruments offer high optical resolution, however, the limited field of view afforded by their objective lenses can mean lengthy read times for some assays, especially where multiple image capture per well is required. Wherever possible, only a small percentage of the total number of cells present in a test well are analysed to keep plate read times at a minimum, which may not always be ideal. Microplate laser- scanning cytometers, such as TTP LabTech’s Acumen® eX3

Stem Cell Derived Human Neurons in CNS Drug Discovery
L Jackson, C Verastegui, C Wells, D Pau, A Nunn, A Finucane, M Lynch, S Boldt, L Gerrard, S.J. MacKenzie

Central nervous system disorders affect many people worldwide. To increase the quality and number of drugs available for treatments of CNS disorders such as Alzheimer’s, schizophrenia and anxiety, the success rate of CNS drug discovery programmes must be improved. The challenge in drug discovery is to develop more relevant assay systems to identify lead compounds for further development. Cell based screening assays currently used for CNS drug development involve primary cells or commercially

Current status and prospects of the commercial storage of adult stem
Alexander E. Rodin, Anatoly N. Gromyko, Alexander B. Smolyaninov

The authors present a result of survey among 300 residents of the Russian Far East on their potential willingness to store cord blood stem cells of their newborns and/or their own gametes for the future needs. The current status of the cord blood banking system in Russia is also described.

Modular Glass Chip System for the Acquisition of the Electric Activity and Physiological Parameters of Differentiated Stem Cells
P. J. Koester, J. Sakowski, S. M. Buehler, C. Tautorat, H. Altrichter, W. Baumann and J. Gimsa

The EU chemical policy REACH, effective on June 2007, regulates the registration, evaluation and authorization of chemicals. Investigations on the effects of neurotoxic and developmental-neurotoxic substances are required by European and US-American test guidelines. However, at the moment these tests are based on animal experiments contradicting society claims to abolish animal experiments and to implement the 3R-principle. The resulting problem is… How to pair REACH and the 3Rs?

Changes in the Gene Expression of mRNA Transcripts for Insulin like Growth Factor, their receptor and Facilitative Glucose Transporter in IVM Oocytes
S.C. Gupta, Neelam Gupta and Alok Pandey

The cDNA libraries from single oocytes and pre-implantation buffalo embryos from 2 cell stage to blastocyst were established. Relative expression studied with RT-PCR of IGF-I showed an increase at 12h and decline at 24h of IVM oocytes. IGF-IR was expressed at cleavage stages to blastocyts. Glut-I was expressed in IVM oocytes and SCNT embryos at all stages. Gene expression of IGF-I, IGF-IR and Glut-I plays an important role in SCNT embryo production.

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Showing Results 61 - 70 of 80
Scientific News
Growing Spinal Disc Tissue
Scientists develop new method for growing spinal disc tissue in the lab for combating chronic back pain.
Elastic Gel to Heal Wounds
A team of bioengineers at Brigham and Women’s Hospital has developed a new protein-based gel that, when exposed to light, mimics many of the properties of elastic tissue, such as skin and blood vessels.
Regenerative Medicine Biologists Discover a Cellular Structure that Explains Fate of Stem Cells
The findings are presented in the journal Nature.
Engineering Yeast that Speaks
Scientists at the University of Washington say they have engineered yeast cells that can "talk" to one another, using the plant hormone auxin.
NuGEN Scientists Screen 400+ Genes for Fusion Events in Single Assay
Breakthrough proves efficacy of new sample preparation method that could accelerate cancer research and development of treatments and diagnostic tests.
Protein Responsible for Blood Vessel Growth in Tumours Discovered
Scientists have discovered a new protein which triggers the growth of blood vessels in breast cancer tumours which have spread to the brain, a common location which breast cancer can spread to.
Epigenetic Switches that turn Stem Cells into Blood Vessel Cells Uncovered
Researchers at the University of Illinois have identified a molecular mechanism that directs embryonic stem cells to mature into endothelial cells.
Human Trials of Manufactured Blood Within Two Years
The first human trials of lab-produced blood to help create better-matched blood for patients with complex blood conditions has been announced by NHS Blood and Transplant.
Cell that Replenishes Heart Muscle Found by UT Southwestern Researchers
Researchers devise a new cell-tracing technique to detect cells that do replenish themselves.
Global Search for Next Antibiotic
University of Queensland researchers have launched a global search to discover antibiotics capable of combating superbug bacteria that are resistant to current antibiotics.
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