Corporate Banner
Satellite Banner
Stem Cells, Cellular Therapy & Biobanking
>
Scientific Community
 
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
Analysing 10,000 Cells Simultaneously
New techniquethat traps 10,000 cells on a single chip has potential for cancer screening for individuals.
Studies Explore Science of Cardiovascular Diseases
Two studies highlight how basic science research insights are key to future treatment breakthroughs.
Stem Cell ‘Heart Patch’ Almost Perfected
Scientists aiming to perfect and test 3D "heart patches" in animal model, last hurdle before human patients.
Stem Cells Growing 3D Lung-in-a-Dish
Researchers have created 3D lung-like tissue from lung-derived stem cells. The tissue can be used to study lung diseases.
MRI Guidance Aids Stem Cell Delivery
Scientists have delivered stem cells to the brain with unprecedented precision, infusing the cells under real-time MRI guidance.
Mechanisms of Parkinson’s Pathology
Defects that lead to cells’ failure to decommission faulty mitochondria cause nerve cells to die, triggering the symptoms of Parkinson’s disease.
Stem Cell Transplant Without Radiation or Chemotherapy
Researchers have successfully performed stem cell transplants without using radiation or chemotherapy.
Advanced Lymphoma in Remission After T-Cell Therapy
63% of trial participants who recieved two-drug combination chemo plus intermediate dose of engineered T cells went into complete remission.
Inherited Heart Condition Breakthrough
Using stem cells, scientists have created a specific heart condition model, yeilding insights into unexpected disease mechanisms.
Biobank Storage Time Affects Blood Test Results
Study finds storage time of blood samples at a biobank may affect test results as much as patient age.
Scroll Up
Scroll Down

Measurement of Proteasome Inhibition in Live Cells in Molecular Devices Microplate Fluorometers
Bookmark and Share

Molecular Devices

Proteins inside eukaryotic cells exist in a dynamic state, in a highly-regulated balance between synthesis and degradation. Whereas protein synthesis is well-understood after decades of study, major advances in our knowledge of protein degradation have occurred only in the last two decades. As a result, the 2004 Nobel prize in chemistry was awarded to Aron Ciechanover, Avram Hershko and Irwin Rose for their discovery of ubiquitin-mediated proteolysis, an ATP-dependent process where unwanted proteins are multiply-tagged with ubiquitin (a 76-amino acid protein).1 The tagged proteins are then transported to the proteasome for degradation. The proteasome is a massive (2.5 MDa), barrel-shaped protein complex inside all eukaryotic cells (and some bacteria) that consists of a tunnel-like core with a cap at each end. (See Figure 1.) The caps (regulatory complexes) recognize and bind targeted proteins and inject them into the central core where the proteins are successively degraded into short peptides. This application note shows that inhibition of proteasome activity can easily be measured using Molecular Devices microplate fluorometers.

Further Information

Related Content

Molecular Devices Forms Scientific Advisory Board
Multidisciplinary team will guide product and application development to deliver maximum value to customers.
Thursday, December 27, 2012
Molecular Insight Pharmaceuticals, Inc. Elects a New Board of Directors
The new Board will support the Company’s strategic focus on the discovery, development, and commercialization of radiopharmaceuticals for molecular imaging and targeted radiotherapy of prostate cancer.
Wednesday, February 29, 2012
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,000+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!