Digital Dispensing for Direct Dilution: New Flexibility in Dose-Response Analyses

HP has been the worldwide leader in inkjet technology for 25 years with HP printers reliably dispensing billions of picoliter droplets per day. The HP T8 Dispensehead is built on a silicon wafer using HP inkjet photolithography to enable precise, accurate drop generation and placement. The single-use dispensehead utilizes 22 high-frequency nozzles so that picoliter to microliter volumes are dispensed in under a second.

Direct dilution using HP picoliter digital dispensing technology was shown to be a replacement for serial dilution. Compound usage can be reduced by 90%, while reliability and speed are improved with simplified workflows. Non-contact dispensing of droplets directly into assay wells make it practical to routinely perform drug-drug interaction experiments. 

This non-contact digital dispensing system frees the researcher from the constraints of analog-based dispensing methodologies, enabling complicated experiments that have not been practical with manual or robotic serial dilution methods.