The MaxDiscovery™ Apolipoprotein B (ApoB) ELISA Kit is an enzyme immunoassay that analyzes the quantity of ApoB in cells, tissues, serum, plasma or cultured cells. Apoliporotein B is the primary apoliporotein of low-density lipoproteins (LDL), which is responsible for carrying cholesterol to tissues. Apoliporotein B acts as a ligand for LDL receptors in various cells throughout the body. The protein has two main isoforms, ApoB-48 and ApoB-100. In humans ApoB-48 is synthesized in the small intestine while ApoB-100 is made in the liver. There is significant evidence that shows that high levels of Apolipoprotein B can lead to plaques that cause atherosclerotic coronary disease. In fact, Apolipoprotein B levels are thought to be an even better indicator of heart disease risk than total cholesterol or LDL. Bioo Scientific’s ApoB ELISA kit is an immunoassay that can be used to measure ApoB levels in cells, serum and tissue with minimal sample preparation. The standard curve spans the range of 0-200 ng/mL. Like most ELISA assays, the MaxDiscovery™ ApoB ELISA Test relies on a Horseradish Perioxidase (HRP) conjugated antibody and the TMB (3,3´,5,5´-tetramethylbenzidine) substrate. TMB is a chromogen that yields a blue color when oxidized with hydrogen peroxide (catalyzed by HRP) that has major absorbances at 370 nm and 652 nm. The color then changes to yellow with the addition of acid with maximum absorbance at 450 nm. The relative amount of ApoB protein in the cells will be directly proportional to the amount of signal that is obtained at 450 nm.