Corporate Banner
Satellite Banner
Scientific Community
Become a Member | Sign in
Home>Products>This Product

MaxDiscovery™ Caspase-1 Detection Kit (Colormetric)

Product Description
The MaxDiscovery™ Caspase-1 Detection Kit (Colormetric) is a colorimetric microplate-based assay to directly measure caspase-1 activation in cultured cells. This kit enables biomedical researchers to determine caspase-1 levels in cultured cells from a broad range of cell lines. The assay utilizes a simple, proven enzymatic assay to detect activated enzyme. The kit is designed to be used with a microplate reader and contains sufficient materials to test 96 samples. The MaxDiscovery™ Caspase-1 Assay Kit measures activated caspase-1 enzyme using a direct, plate-based, colorimetric reaction. The kit contains a substrate solution containing a caspase-specific tetrapeptide substrate, ac-Tyr-Val-Ala-Asp-pNA. The zymogen procaspase-1 has no reactivity towards this substrate. Active caspase-1 specifically cleaves the pNA group from the peptide moiety. The free pNA group has a yellow color, which strongly absorbs light at 405 nm; the color is also visible in the wells. Mammalian cells are growth in culture wells and treated. The treated cells are lysed in the wells (after removing the cell culture medium) using a cell lysis buffer provided with the kit. The lysate is then directly added to wells in a 96-well reaction microplate (provided) containing the ac-Asp-Glu-Val-Asp-pNA substrate solution. Caspase-1 activity is then measured using a plate reader for the increase in absorbance at 405 nm in 30 minutes.
Product MaxDiscovery™ Caspase-1 Detection Kit (Colormetric)
Company BIOO Scientific - Product Directory
Price Request a quote
More Information View company product page
Catalog Number 5812
Quantity 1 x 96 wells
Company Logo

BIOO Scientific - Product Directory
3913 Todd Lane Suite 312 Austin, TX 78744, USA

Tel: +1 512-707-8993
Fax: +1 512-707-8122

Scientific News
Salford Lung Study - The First Real World Clinical Trial
In this podcast, we learn about the Salford Lung Study and its potential to revolutionize the way we assess new drugs and treatments around the world.
Study Finds Key Regulator in Pulmonary Fibrosis
Researchers identify an enzyme that could open the way to therpies for chronic fatal lung disease.
Signaling Pathway Could Be Key to Improved Osteoporosis Treatment
Inhibition of SIK2 enzyme both stimulates bone formation and reduces bone breakdown in animal model.
Ovarian Cancer Insight
Study showed tumours release cytokines to attract macrophages, which secrete growth factors that in turn promote tumour growth.
Substance Can Potentially Postpone Aging
Researchers bridge the gap between two main aging theories - repairs to the DNA and poor functioning mitochondria.
Untangling Cause Of Memory Loss In Neurodegenerative Diseases
NIH-funded mouse study identifies a possible therapeutic target for a family of disorders.
New Model for Understanding Human Myeloma
Researchers develop mouse model where mice carry six human genes involved in human tumour growth.
Preventing Alzheimer's in Mice
Researchers have prevented the Alzheimer’s development in mice by using a virus delivery system to transport a specific gene into the brain.
New Platform for Roundworms Could Speed Up Drug Delivery
Researchers have developed a large-scale in vitro drug discovery platform that could speed up scientific research.
"Junk" DNA Critical for Heart Function
Loss of noncoding elements of genome, known as enhancers, results in abnormalities of heart functions.


SELECTBIO Market Reports
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,200+ scientific videos