Corporate Banner
Satellite Banner
Scientific Community
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
Enzyme Malfunction May be Why Binge Drinking Can Lead to Alcoholism
A new study in mice shows that restoring the synthesis of a key brain chemical tied to inhibiting addictive behavior may help prevent alcohol cravings following binge drinking.
Developing a Gel that Mimics Human Breast for Cancer Research
Scientists at the Universities of Manchester and Nottingham have been funded to develop a gel that will match many of the biological structures of human breast tissue, to advance cancer research and reduce animal testing.
Lung Repair and Regeneration Gene Discovered
New role for hedgehog gene offers better understanding of lung disease.
New Gene Therapy for Vision Loss From a Mitochondrial Disease
NIH-funded study shows success in targeting mitochondrial DNA in mice.
Self-Propelled Powder to Stop Bleeding
UBC researchers have created the first self-propelled particles capable of delivering coagulants against the flow of blood to treat severe bleeding, a potentially huge advancement in trauma care.
Tension Helps Heart Cells Develop Normally in the Lab
Stanford engineers have uncovered the important role tension plays in growing heart cells out of the body.
Controlling Body Temperature in Response to 'Fight or Flight'
New research in The FASEB Journal suggests that blocking TRPV1 protein causes an increased release of noradrenaline, leading to an increase in core body temperatures.
Inroads Against Leukaemia
Potential for halting disease in molecule isolated from sea sponges.
Researchers Disguise Drugs As Platelets to Target Cancer
Researchers have for the first time developed a technique that coats anticancer drugs in membranes made from a patient’s own platelets.
A Fundamental Protection Mechanism Against Formalin In Mammals is Revealed
Formaldehyde, or formalin, is well known to all of us as a common chemical used in many industrial processes and also as a preservative, remarkably we also produce formaldehyde in our bodies.
Scroll Up
Scroll Down

Rapid Critical Micelle Concentration (CMC) Determination Using Fluorescence Polarization
Bookmark and Share

BioTek Instruments

This application note describes the rapid semi-automated determination of CMC values for surfactants in 384-well microplates using fluorescence polarization. 

These data demonstrate that the use of DAF fluorescence polarization as a means to determine the CMC values for surfactants is not only easy and accurate, but that the method can also be easily scaled for large sample numbers. Unlike fluorescence intensity, fluorescence polarization uses a ratio of two measurements on each well, correcting for differences in intensity brought about by experimental conditions, such as pH, temperature, and surfactant concentration. The Synergy Neo reader is a high throughput reader specifically designed for the measurement of large numbers of samples. 

The reader uses modular optic cubes to measure numerous read modalities, which include UV-Vis absorbance, luminescence, fluorescence intensity, time resolved fluorescence, HTRF®, AlphaScreen®, and fluorescence polarization. In regards to fluorescence polarization, the reader is capable of simultaneously determining parallel and perpendicular measurements. Gen5™ software (BioTek instruments) not only controls reader function, but also is capable of automatically performing the 4-parameter logistic fit and calculating CMC values.

Further Information

Related Content

BioTek Instruments and Global Cell Solutions set to Collaborate
BioTek Instruments, Inc., and Global Cell Solutions, Inc., (GCS) announce a formal collaboration for the coming year at LabAutomation 2010. The poster Automation of a Microplate Cell-based Assay to Measure Activity of the Histamine H1 G Protein-coupled Receptor Using a Novel 3-D Cell Culture Technique is the first in a series of collaborative publications detailing the use of BioTek’s microplate instrumentation for various cell-based assays.
Wednesday, January 27, 2010

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,600+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,800+ scientific videos