Corporate Banner
Satellite Banner
Scientific Community
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
Developing a Gel that Mimics Human Breast for Cancer Research
Scientists at the Universities of Manchester and Nottingham have been funded to develop a gel that will match many of the biological structures of human breast tissue, to advance cancer research and reduce animal testing.
Lung Repair and Regeneration Gene Discovered
New role for hedgehog gene offers better understanding of lung disease.
New Gene Therapy for Vision Loss From a Mitochondrial Disease
NIH-funded study shows success in targeting mitochondrial DNA in mice.
Self-Propelled Powder to Stop Bleeding
UBC researchers have created the first self-propelled particles capable of delivering coagulants against the flow of blood to treat severe bleeding, a potentially huge advancement in trauma care.
Tension Helps Heart Cells Develop Normally in the Lab
Stanford engineers have uncovered the important role tension plays in growing heart cells out of the body.
Controlling Body Temperature in Response to 'Fight or Flight'
New research in The FASEB Journal suggests that blocking TRPV1 protein causes an increased release of noradrenaline, leading to an increase in core body temperatures.
Inroads Against Leukaemia
Potential for halting disease in molecule isolated from sea sponges.
Researchers Disguise Drugs As Platelets to Target Cancer
Researchers have for the first time developed a technique that coats anticancer drugs in membranes made from a patient’s own platelets.
A Fundamental Protection Mechanism Against Formalin In Mammals is Revealed
Formaldehyde, or formalin, is well known to all of us as a common chemical used in many industrial processes and also as a preservative, remarkably we also produce formaldehyde in our bodies.
Drug Used To Treat HIV Linked to Lower Bone Mass in Newborns
NIH study finds mothers’ use of tenofovir tied to lower bone mineral content in babies.
Scroll Up
Scroll Down

3D cell culture: a realistic and reproducible collagen-based environment
Bookmark and Share

Tecan Group

In drug discovery and many other areas of research, cell culture using primary cells of human or animal origin is often desirable, or even essential. TAP Biosystems has developed a novel 3D cell culture technique, RAFT (Real Architecture for 3D Tissue), which uses the most abundant matrix protein in the body: type I collagen. 

This technology allows researchers to culture cell types of their choice, including primary cells, in an in vivo like environment that is essentially a hydrogel with tissue-like properties. Uniquely, the RAFT process increases the collagen concentration to physiological levels rapidly and reproducibly, while maintaining high cell viability. 

Tecan and TAP Biosystems have developed an automated process for the preparation of RAFT cell cultures, aiding mixing procedures and enhancing reproducibility while maintaining excellent cell viability and reliability. Implementation on the Freedom EVO system, with its modular automation capabilities, increases experimental versatility and analytical flexibility. 

This application note presents cell viability and proliferation data for primary neonatal human dermal fibroblasts (Life Technologies) obtained over an 11-day culture period after seeding.

Further Information

Related Content

Joining the Digital Age
TES Pharma has recently purchased an HP D300 Digital Dispenser, and is now reaping the benefits of direct titration with improved assay reproducibility and more time to perform exploratory research.
Thursday, February 21, 2013
Tecan and Pion Expand Collaboration to Develop Automated Drug Screening Solutions
The new automated application provides scalability from an entry-level manual system to a fully automated system with medium or high throughput.
Thursday, July 16, 2009
A perfect fit for the Freedom EVO® 75 in forensic toxicology
Immunalysis Corporation tailors the Tecan Freedom EVO 75 liquid handing workstation as the platform of choice to provide customized semi and fully automated drugs of abuse screening solutions.
Friday, March 14, 2008

Skyscraper Banner
Go to LabTube
Go to eposters
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,600+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,800+ scientific videos