Corporate Banner
Satellite Banner
Technology Networks Header
Thursday, October 02, 2014
Technology Networks
 
Register | Sign in
Home Page > Videos > 3D Organotypic Liver Cultures and their Application in Predictive Toxicology
  Videos

Return

3D Organotypic Liver Cultures and their Application in Predictive Toxicology
SELECTBIO

3D cultivation allows organotypic microtissue formation ensuring cell-cell contacts and contacts to the extracellular matrix. The communication between cells at tight junctions and across extracellular matrix induces and maintains cellular differentiation, functionality and viability. 3D cell cultivation techniques can tremendously improve studies on drug metabolism, drug toxicity especially long-term repeated dose effects. Using a high-throughput 3D cultivation system, we produced 3D organotypic cultures of human hepatic cells using a 96 well plate based hanging drop method. This method allows scaffold-free reorganization of cells under the force of gravity. We show that the production of 3D organotypic cultures of various hepatic cell lines and primary liver cells is possible (Mueller et al., 2011, 2013). The hepatic cells in these 3D cultures were analyzed for viability and functionality. We show that the organotypic cultures maintain high liver-specific function over 3 weeks of culture (Gunness et al., 2013). The effects of several compounds (acetaminophen, aflatoxin B, valproic acid, chlorpromazine, troglitazone and rosiglitazone) were monitored and compared. Currently we are using this system for mechanistic studies. In conclusion, better functionality of 3D systems improves the prediction of toxicity and will have impact in the changed paradigm of drug screening.

Request more information
Company product page



For access to this article, enter your email address to instantly recieve a Password Reset link.

Please enter your email address below:

Existing users please Sign In here. Don't have an account? Register Here for free access.

Don't have an account? | Register Here

Scientific News
UW-Madison Team Developing ‘Tissue Chip’ to Screen Neurological Toxins
A faster, more affordable way to screen for neural toxins.
Promise for Treatment of Pancreatic Cancer
Ultrasound microbubbles can improve tumor absorption of cancer drugs.
Animal Testing can Mislead Drug Discovery and Development
Several blockbuster drugs would not be on the market, if scientists had relied solely on drug-uptake in animal trials, according to new research.
Marijuana Compound May Offer Treatment for AD
Extremely low levels of the compound in marijuana known as delta-9-tetrahydrocannabinol, or THC, may slow or halt the progression of Alzheimer’s disease.
New Advances in the Pre-clinical Evaluation of Hepatitis B Therapies
Imperial College London to present first public HBV Data from CN Bio in vitro liver model.
ADME of Antibody-Maytansinoid Conjugates
Continued understanding of the ADME properties of ADCs entering clinical evaluations should provide additional insight into attributes that may be necessary for clinical success.
Epigenetic Regulation of ADME-Related Genes
The promise of epigenetic research is its potential to identify additional sources of inter individual variability in drug disposition and response.
NIST Instrument Enables High-speed Chemical Imaging of Tissues
Researchers have demonstrated a dramatically improved technique for analyzing biological cells and tissues based on characteristic molecular vibration "signatures."
Finding Could Revolutionize Drug Discovery
A study by researchers at IRB Barcelona reveals the existence of information highways that connect and correlate distant sites within a single protein.
Researchers Develop ‘Onion’ Vesicles for Drug Delivery
University of Pennsylvania researchers have shown that dendrimer-based vesicles self-assemble with concentric layers of membranes, much like an onion.
Scroll Up
Scroll Down
Skyscraper Banner
Skyscraper Banner
Follow TechNetcom1 on Twitter
Technology Networks Ltd. on LinkedIn
Go to LabTube.tv