Corporate Banner
Satellite Banner
ADME Tox
Scientific Community
 
Join | Sign in
Home>Videos>This Video
  Videos

Return

3D Organotypic Liver Cultures and their Application in Predictive Toxicology
SELECTBIO

3D cultivation allows organotypic microtissue formation ensuring cell-cell contacts and contacts to the extracellular matrix. The communication between cells at tight junctions and across extracellular matrix induces and maintains cellular differentiation, functionality and viability. 3D cell cultivation techniques can tremendously improve studies on drug metabolism, drug toxicity especially long-term repeated dose effects. Using a high-throughput 3D cultivation system, we produced 3D organotypic cultures of human hepatic cells using a 96 well plate based hanging drop method. This method allows scaffold-free reorganization of cells under the force of gravity. We show that the production of 3D organotypic cultures of various hepatic cell lines and primary liver cells is possible (Mueller et al., 2011, 2013). The hepatic cells in these 3D cultures were analyzed for viability and functionality. We show that the organotypic cultures maintain high liver-specific function over 3 weeks of culture (Gunness et al., 2013). The effects of several compounds (acetaminophen, aflatoxin B, valproic acid, chlorpromazine, troglitazone and rosiglitazone) were monitored and compared. Currently we are using this system for mechanistic studies. In conclusion, better functionality of 3D systems improves the prediction of toxicity and will have impact in the changed paradigm of drug screening.

Request more information
Company product page


Access to this article and other content is for registered users.

Join the Technology Networks Community

  • Access to the latest scientific news, products and research through Technology Networks
  • Upload and share your posters on ePosters
  • View a library of 1,800+ scientific and medical posters
  • A library of 3,000+ scientific videos on LabTube


Sign In



Forgotten your details? Click Here
If you already have an account with Technology Networks, please use your existing login details. If you do not yet have an account please join here.

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Scientific News
Bacteria Cooperate to Repair Damaged Siblings
New research unearths the unique ability of a certain type of soil borne bacteria to repair nearby damaged cells.
Vitamin E Keeps Muscles Healthy
Body builders have it right: vitamin E does help build strong muscles, and scientists appear to have figured out one important way it does it.
Microchip Captures Clusters of Circulating Tumor Cells
Researchers have developed a microfluidic chip that can capture rare clusters of circulating tumor cells, which could yield important new insights into how cancer spreads.
New Method For Measuring The Concentration Of Medicines In The Blood
Researchers at EPFL have come up with a quick and portable new device for measuring the amount of medicinal drugs in blood.
Label-Free Binding Analysis Trends
Understand current trends and learn which application areas are expected to gain in popularity over the next few years.
Alternate Administration Routes for Psychotropic Drugs Reviewed
A review in the Annals of Pharmacotherapy has examined the literature on alternative routes of administration for antipsychotic and antidepressant medications.
Promising Results of Phase 1/2 Trial of Rociletinib in EGFR Lung Cancer Published
Results have been published of a multi-center phase I/II study of the investigational anti-cancer agent rociletinib in patients with EGFR-mutant non-small cell lung cancer (NSCLC) that had progressed after previous treatment with EGFR inhibitors.
Epilepsy Drug Metabolism Test Launched for Epilepsy and Seizure Disorders
Courtagen Life Sciences, Inc have announced the release of a NGS test focused on detection of anti-epileptic drug (AED) compatibilities and interactions for patients receiving treatment for epilepsy or other seizure disorders.
Supercooling May Aid Drug Delivery
A new study published is the first to break down the rules governing the complex process of crystallization through rapid-cooling.
Quotient Clinical Completes Innovative First-In-Human Program
First-in-human clinical program designed to develop an optimal oral formulation.
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters