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Advanced Cell Culture Systems...Contract Assay Services at STEMCELL Tech...DeNovix DS-11 FX Spectrophotometer / Flu...

Featured Products
Featured ProjectNutriStem® hPSC XF Medium A Superior Xeno-free, Serum-free Culture Medium for hES and hiPS Cells
NutriStem® hPSC XF Medium A Superior Xeno-free, Serum-free Culture Medium for hES and hiPS Cells
Featured ProjectWhitley A95 Workstation
Whitley A95 Workstation

Webcasts
The Tip of the Iceberg - How Pipette Tips Influence Assay ResultsThe Tip of the Iceberg - How Pipette Tips Influence Assay Results
n this webinar, you will learn everything you need to know about pipette tips and the huge impact they can have on your results.
Subcellular fractionation and enrichment strategies for improved mass spectrometry analysisSubcellular fractionation and enrichment strategies for improved mass spectrometry analysis
In this webinar, you will learn how differential detergent subcellular fractionation can improve protein identification and be used to identify co-localizing proteins.

Market Reports
3D Spheroid Culture Trends 2015
HTStec Limited

This market report summarizes the results of HTStec’s industry-wide global web-based benchmarking survey on three dimensional (3D) spheroid culture carried out in May 2015.
Immunoassay Auto Trends 2014
HTStec

This report comprehensively documents current practices and preferences in immunoassays and to understand future user requirements, particularly with respect to immunoassay automation.
Cell-Based Label-Free Detection Trends 2014
HTStec Limited

This market report summarizes the results of HTStec’s 4th global web-based benchmarking survey on cell-based label-free (LF) detection carried out in July and August 2014. The study also examined in detail the current market landscape, application areas and interest in purchasing new products/systems.
Scientific News
Featured Article
Stem Cells in Drug Discovery
Potential Source of Unlimited Human Test Cells, but Roadblocks Remain.

More Effective Strategy for Producing Flu Vaccines
Researchers have developed a virus backbone, allowing producers to grow vaccine viruses in mammalian cells, rather than in eggs.
Inspiring Futuristic Innovation: Brain ‘Organoids’
Scientists create artificial brains, providing an advanced model for studying brain tumour development.
Tissue Damage Is Key for Cell Reprogramming
Researchers have shown tissue damage is important for cells to return to an embryonic state for cell reprogramming.
Making Personalized Medicine a Reality
Groundbreaking technique developed at McMaster University is helping to pave the way for advances in personalized medicine.
Regenerating Diseased Hearts
Researchers from the University of Otago have probed the potential of adult stem cell types to repair diseased hearts.
Using Cancer Cells' Mass to Predict Treatment Response
A device has been developed that can detect changes in cell mass at a minute scale.
Color-Coded Stem Cells
Researchers develop colour-coding tool for tracking live blood stem cells over time.
Human Intestines and Functioning Nerves Engineered
The new technology enables the study of human health and advances the goal of regenerative medicine.
Chemical Snapshots Could Lead to Better Engineered Cartilage
Taking "chemical photographs" of the cartilage between joints and comparing it to engineered versions could lead to better implants, say researchers.
Business News
Eppendorf Acquires Calibration Technology
Acquisition will allow Eppendorf to expand it's service presence into Ireland.
Plasticell Completes £1.32 Million Financing to Advance Therapeutic Projects
Plasticell announces the completion of a two-part equity financing round, raising a total of £1.32 million from its shareholders and new investors.
InSphero Scientist Receives 3Rs Award for Microtissue-based Body on a Chip System
Dr. Olivier Frey has received the 2016 IQ Consortium and AAALAC International Global 3Rs Award for work on MPS to reduce animal-based drug testing.
Changes in the Management Board of Eppendorf AG
Changes include the creation of a new Board position which will include Sales, Marketing and Service in order to focus on developing the market presence of Eppendorf.
Selexis, ImmuNext Announces Cell Line License Agreement
Relates to anti-CD40L antibody being developed for treatment of chronic autoimmune disorders.
Greenovation´s BryoTechnology Substantially Improves Yields of Pharmaceutical Proteins
The recent yield improvements once again underline the viability of moss-based cGMP-production as a beneficial alternative to mammalian cells in steel-tanks.
smartLAB 2017: For Tomorrow’s Intelligent Laboratories
smartLAB 2017 will be held 16-18 May at LABVOLUTION along side BIOTECHNICA life sciences show.
Experts Assemble for Inaugural 3D Cell Culture Conference
Event draws over 100 leading government, industry, and academic leaders to further translational applications of 3D tissue models.
Horizon Signs Licensing Agreement for Biomanufacturing Cell Lines
Under the agreement, the Partner will gain immediate access to one of Horizon’s cutting-edge engineered cell lines for an indefinite period of time in order to manufacture their novel therapeutics.
ATCC, BioAgilytix Partner
Partnership aims to develop custom cell-based assay solutions to support biopharmaceutical research and development.

Events
3D Cell Culture: Innovations i
07 Feb 2017
3D Cell Culture: Innovations i ...
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Cell Culture & Downstream Worl
21 Feb 2017
Cell Culture & Downstream Worl ...
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3D Cell Culture 2017
22 Feb 2017
3D Cell Culture 2017 ...
More Information
Probiotics Congress: Asia
01 Mar 2017
Probiotics Congress: Asia ...
More Information

ePosters
Microfluidic extrusion of cell-laden hydrogel fibers for 3D-Bioprinting
CLNS - iit@Sapienza - Viale Regina Elena 291 - 00161 - Roma

An overview on how the incorporation of microfluidic platforms in 3D-bioprinters can open new routes in the creation of heterogeneous, tissue-specific bio-constructs and in the high-throughput screening of bioinks compositions for regenerative medicine and in vitro tissue modeling.
The Chemical Synthesis of Long and Highly Modified RNA using 2'-ACE Chemistry
GE Healthcare Dharmacon, Inc.

Dharmacon has previously developed a novel RNA synthesis chemistry making RNA synthesis as reliable, accessible and of comparable quality as routinely observed in DNA synthesis.
Experimental design considerations for efficient and specific gene knockin using a CRISPR-Cas9 for HDR with synthetic crRNA and tracrRNA
Dharmacon, part of GE Healthcare

Precise genome engineering with CRISPR-Cas9 and single-stranded DNA oligo or double-stranded DNA plasmid donors via homology-directed repair (HDR).
Comparison of Key Signaling Pathways in Lung Cancer Cells Grown in 2D and 3D Formats
Western University of Health Sciences

Evaluation of Wnt/ß-catenin and BMP pathway proteins and gene expression in NSCLC cell lines A549 and H460 in 2D and 3D cultures.

Exosomes promote survival in squamous head and neck cancer cells after ionizing radiation
Helmholtz Zentrum München, Institute of Radiation Biology, Neuherberg, Germany / Department of Chemistry, Technical University of Munich, Munich, Germany

Exosomes are important intercellular communicators in the acute radiation stress-response. These nanometer-sized extracellular vesicles confer protective signals to neighboring cells and therefore promote the tumorigenic and radioresistant phenotype of head and neck cancer cells.
Use of non-modified RNAs for the derivation of clinically-relevant iPS cell lines from human blood, urine and skin cells using GMP-compliant reagents
Stemgent, Lexington, MA, USA; Translational Oncology at Univ Medical Center, Mainz, Germany, BioNTech RNA Pharmaceuticals GmbH, Mainz, Germany; Division of Respiratory Medicine, University of Cambrid

We have developed protocols using our StemRNA-NM Reprogramming Kit for generating iPS cells from skin-derived fibroblasts, blood-derived EPCs, and urine-derived UDCs using GMP-compatible reagents (NutriStem medium, iMatrix-511 recombinant laminin). These protocols generate GMP-compatible stem cells without the use of feeders, conditioned medium, additional small molecules or proteins, or passaging during reprogramming.
A Novel Set of Serum-Free, Xeno-Free Differentiation Media for Adipogenesis, Osteogenesis and Chondrogenesis of Human Mesenchymal Stem Cells from Various Tissue Sources
Biological Industries

An overview of a novel SF, XF differentiation system which enables achieving defined conditions for rapid generation of differentiated hMSCs towards tissue engineering and drug screening applications.
Super-resolution single molecule localization microscopy of the exocytotic machinery underlying insulin secretion.
Heriot-Watt Univeristy

Single molecule imaging of the tSNARE proteins involved in insulin secretion.
Quantitative Cell-Based Bioassays for Individual and Combination Immune Checkpoint Immunotherapy Targets
Promega Corporation

Immune checkpoint receptors are promising new immunotherapy
targets for the treatment of a variety of diseases including cancer and
autoimmune-mediated disorders. We developed a suite of cell-based
bioluminescent reporter bioassays for individual and combination
immune checkpoint immunotherapy targets including: PD-1 (PD-L1 or PD-L2), CTLA-4, LAG-3, TIGIT, PD-1+TIGIT, GITR, 4-1BB, CD40, and OX40.

A Novel Set of Serum-Free, Xeno-Free Differentiation Media for Adipogenesis, Osteogenesis and Chondrogenesis of Human Mesenchymal Stem Cells from Various Tissue Sources
Biological Industries

An overview of a novel SF, XF differentiation system which enables achieving defined conditions for rapid generation of differentiated hMSCs towards tissue engineering and drug screening applications
Application Notes
Monitoring Saccharomyces Cerevisiea Growth with Bright Field Microscopy in Real Time
BioTek Instruments, Inc.

Strains of the yeast Saccharomyces cerevisiea serve critical roles in the production of many different products including food staples, medicines, and biofuels. The ability to obtain the desired product reliably and repeatedly requires careful monitoring of not only the input materials, but the growth of the yeast strain during the process.
Multipette®/ Repeater® M4 Allows for Fast, Precise and Sterile Liquid Transfer in Cell Culture
Eppendorf AG

This application note demonstrates the huge advantages offered by Multipette M4 in terms of accuracy and precision needed for many cell culture applications.
Aseptic Identification of Polysorbates using Handheld Raman
Rigaku Raman Technologies

As polysorbates are reactive to light and air, packaging is usually in thick, dark, amber bottles. This makes the identification process more difficult, as opening the container could compromise the sterility and quality of these materials. Handheld Raman is capable of identifying these materials without any sampling or preparation.
Cell Culture Media Identification In Biopharmaceuticals With Handheld Raman
Rigaku Raman Technologies

This application note discusses the identification of cell culture media using Raman analysis.
Performing ADCC Reporter Bioassay with an Environmentally Controlled Microplate Reader
Tecan Group Ltd.

The Promega ADCC Reporter Bioassay provides effector and target cells in frozen, thaw-and-use format, removing the need for isolating or culturing cells in order to perform the assay. Reporter induction requires a six-hour to overnight incubation in a standard cell culture incubator. The CO2 and temperature control features of the Tecan Infinite® 200 PRO with GCM allow the assay induction to be performed without the requirement for a cell culture incubator.
3D Cell Culture: mimicking real tissue
Tecan Group Ltd.

Technological advances in the last couple of years have enabled 3D scaffolds to be engineered that offer a real proxy for the in vivo environment which can be easily and routinely used in a cell culture laboratory. Alvetex Scaffold enables cells to develop natural, in vivo-like intercellular interactions, providing an ideal environment for real three-dimensional growth and nutrient exchange that is comparable to intra-capillary exchange in living tissues.
3D cell culture: a realistic and reproducible collagen-based environment
Tecan Group

Researchers are increasingly turning to 3D cell culture techniques, due to the improved physiological relevance of the cellular environment. 3D cell cultures offer a more in vivo-like response to stimuli more closely resembling the behavior of animal models or the human body.
Optimized cell culture
Tecan Group Ltd.

Culturing of various stable cell lines, including bacterial, yeast, hybridoma, insect and mammalian lines, and stem cells, is an important task in the pharmaceutical industry. The cells need to be maintained and expanded for use in cell-based assays, such as siRNA screening, ADME and high content screening, as well as for clone selection.
Determination of microbial cell viability
Tecan Group Ltd.

The BacTiter-Glo Microbial Cell Viability Assay is a luminescence-based assay for the determination of the viability of microbial cells, using quantification of ATP as an indicator of metabolically active, viable cells. The assay protocol comprises the addition of the BacTiter-Glo reagent directly to the sample, and subsequent measurement of luminescence; cell washing, removal of culture medium, and multiple pipetting steps are not required.
Stem cell research: Creating blood for high throughput screening
Tecan Group Ltd.

Many different research fields, including investigation of early human development processes, disease research, drug discovery and cellular therapy applications, require appropriate in vitro models which can accurately mimic in vivo processes to provide biologically relevant results. Pluripotent stem cells (PSCs) offer a powerful system for creating in vitro models to address these questions.
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