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The Fight Against Food Fraud: The Analyt...
Learn to pipette like a pro...Behind the Science, episode 7: Get Relax...Contract Assay Services at STEMCELL Tech...

Featured Products
Featured ProjectIncuCyte® Live-Cell Imaging and Analysis System from Essen BioScience
IncuCyte® Live-Cell Imaging and Analysis System from Essen BioScience
Featured ProjectSensitive detection with best-in-class performance
Sensitive detection with best-in-class performance

Webcasts
Preventing Contamination in Cell Culture LabsPreventing Contamination in Cell Culture Labs
Join us to learn how to apply aseptic techniques and preventative hygiene for preventing contamination by incorporating standard procedures into your daily culture practice.
SiLA—Standards to Improve Overall Lab EfficiencySiLA—Standards to Improve Overall Lab Efficiency
This talk covers how SiLA (Standardization in Lab Automation) makes laboratory integration simpler today and in the future.

Market Reports
3D Spheroid Culture Trends 2015
HTStec Limited

This market report summarizes the results of HTStec’s industry-wide global web-based benchmarking survey on three dimensional (3D) spheroid culture carried out in May 2015.
Immunoassay Auto Trends 2014
HTStec

This report comprehensively documents current practices and preferences in immunoassays and to understand future user requirements, particularly with respect to immunoassay automation.
Cell-Based Label-Free Detection Trends 2014
HTStec Limited

This market report summarizes the results of HTStec’s 4th global web-based benchmarking survey on cell-based label-free (LF) detection carried out in July and August 2014. The study also examined in detail the current market landscape, application areas and interest in purchasing new products/systems.
Scientific News
Emerging Model of Cancer
Cancer acts cooperatively, making individual decisions but acting in unison; this insight is being used to create a computer model of cancer.
Characterizing the Mouse Gut Microbiome
Study establishes the first public collection of bacteria from the intestine of mice.
How Cloud Connectivity Can Combat the Reproducibility Crisis
This infographic explains the reproducibility crisis, and how cloud connectivity can help overcome this problem.
Stem Cells Grown On Scaffold Mimic Hip Joint Cartilage
Adult fat-derived stem cells grown on a 3-D scaffold that mimicked a hip joint surface formed cartilage and maintained the correct shape.
'Fixing' Blood Vessel Cells To Diagnose Blood Clotting Disorders
A novel microfluidic device detects how endothelial cells that line vessels contribute to hemostasis.
Using Animal Embryos Containing Human Cells
With recent advances in stem cell and gene editing technologies, an increasing number of researchers are interested in growing human tissues and organs in animals by introducing pluripotent human cells into early animal embryos.
Engineering Cells to Speed Tissue Repair
Kelly Schultz studies how cells remodel their microenvironment - a crucial step toward engineering cells to move through synthetic material for faster tissue regeneration.
Cancer Cells Migrate Towards Oxygen
Bioengineers report results showing sarcoma cells in mice finding pathways toward higher concentrations of oxygen
Biopharmaceuticals on Demand
Portable production system would use microbes for manufacturing small amounts of vaccines and therapeutics.
Singapore Scientists Grow Mini Human Brains
Mini midbrains provide next generation platforms to investigate human brain biology, diseases and therapeutics
Business News
IBO Announces Industrial Design Award Winners
IBO announced their Industrial Design Awards winners in three separate award categories.
Eppendorf Continues on Course for Success
Eppendorf has recorded group sales in first half-year grew 5.5% and income from opperation climbing 23.3%.
Plasticell, NUI Galway Collaborate
Plasticell has announced the signing of a collaboration agreement with the National University of Ireland, Galway focused on methods of eradicating cancer stem cells.
Kite Pharma, University of California Announce Collaboration
Collaboration aims to advance development of off-the-shelf allogeneic T-cell therapies from renewable pluripotent stem cells.
ASLAN, Selexis Announce Agreement
Collaboration aims to develop a CHO-K1 cell line for expression of ASLAN004.
Axol, Metrion Announce Research Collaboration
The human cell culture specialists join experts in ion channel research to study human iPSC-derived cardiomyocytes for drug discovery applications.
Turgut Ilaclari, Selexis Announce Agreement
Collaboration aims to progress development of biosimilar products for the treatment of inflammatory diseases.
Registration Open for New Frontiers in 3D Cell Culture-based Screening Technologies Conference
Inaugural meeting, organized by InSphero AG, NCATS, CAAT, and Promega Corp., to be held Thursday, October 13, 2016 in Baltimore, MD.
International Collaboration to Create New Cancer Models
An international project creating new cancer models has been formed to help accelerate cancer research
API, Swansea University Announce Agreement
Collaboration aims to develop 3D printed tissue cartilage made from human cells and nanocellulose for use in facial reconstruction.

Events
Moving forward with stem cell
06 Sep 2016
Moving forward with stem cell ...
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EMBO|EMBL Symposium: Actin in
07 Sep 2016
EMBO|EMBL Symposium: Actin in ...
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Cell Culture & Cell Line Devel
12 Sep 2016
Cell Culture & Cell Line Devel ...
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EMBL–Wellcome Genome Campus Co
14 Sep 2016
EMBL–Wellcome Genome Campus Co ...
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ePosters
Comparison of Key Signaling Pathways in Lung Cancer Cells Grown in 2D and 3D Formats
Western University of Health Sciences

Evaluation of Wnt/ß-catenin and BMP pathway proteins and gene expression in NSCLC cell lines A549 and H460 in 2D and 3D cultures.

Exosomes promote survival in squamous head and neck cancer cells after ionizing radiation
Helmholtz Zentrum München, Institute of Radiation Biology, Neuherberg, Germany / Department of Chemistry, Technical University of Munich, Munich, Germany

Exosomes are important intercellular communicators in the acute radiation stress-response. These nanometer-sized extracellular vesicles confer protective signals to neighboring cells and therefore promote the tumorigenic and radioresistant phenotype of head and neck cancer cells.
Use of non-modified RNAs for the derivation of clinically-relevant iPS cell lines from human blood, urine and skin cells using GMP-compliant reagents
Stemgent, Lexington, MA, USA; Translational Oncology at Univ Medical Center, Mainz, Germany, BioNTech RNA Pharmaceuticals GmbH, Mainz, Germany; Division of Respiratory Medicine, University of Cambrid

We have developed protocols using our StemRNA-NM Reprogramming Kit for generating iPS cells from skin-derived fibroblasts, blood-derived EPCs, and urine-derived UDCs using GMP-compatible reagents (NutriStem medium, iMatrix-511 recombinant laminin). These protocols generate GMP-compatible stem cells without the use of feeders, conditioned medium, additional small molecules or proteins, or passaging during reprogramming.
A Novel Set of Serum-Free, Xeno-Free Differentiation Media for Adipogenesis, Osteogenesis and Chondrogenesis of Human Mesenchymal Stem Cells from Various Tissue Sources
Biological Industries

An overview of a novel SF, XF differentiation system which enables achieving defined conditions for rapid generation of differentiated hMSCs towards tissue engineering and drug screening applications.
Super-resolution single molecule localization microscopy of the exocytotic machinery underlying insulin secretion.
Heriot-Watt Univeristy

Single molecule imaging of the tSNARE proteins involved in insulin secretion.
Quantitative Cell-Based Bioassays for Individual and Combination Immune Checkpoint Immunotherapy Targets
Promega Corporation

Immune checkpoint receptors are promising new immunotherapy
targets for the treatment of a variety of diseases including cancer and
autoimmune-mediated disorders. We developed a suite of cell-based
bioluminescent reporter bioassays for individual and combination
immune checkpoint immunotherapy targets including: PD-1 (PD-L1 or PD-L2), CTLA-4, LAG-3, TIGIT, PD-1+TIGIT, GITR, 4-1BB, CD40, and OX40.

A Novel Set of Serum-Free, Xeno-Free Differentiation Media for Adipogenesis, Osteogenesis and Chondrogenesis of Human Mesenchymal Stem Cells from Various Tissue Sources
Biological Industries

An overview of a novel SF, XF differentiation system which enables achieving defined conditions for rapid generation of differentiated hMSCs towards tissue engineering and drug screening applications
Phenotypic Cell-Based Screening of a High Content Imaging Cell Health Assay using the IN Cell Analyzer 2000
MRC Technology

A High Content Imaging Cell Health assay has been established in-house for phenotypic High Content Screening using the IN Cell Analyzer 2000 and Genedata Screener analysis software. We describe the assay development and initial screening of the FDA set in HepG2 cells for validation of the assay.
Analyzing Cell Viability in 3D Tissue Models with the ViaLight™ Plus BioAssay
1Lonza Cologne GmbH, Cologne, Germany; 2Lonza Walkersville Inc., Walkersville, MD, USA; 3Lonza Rockland Inc., Rockland, ME, USA

This poster explains how to measure cell viability easily in 3D cell cultures using the ViaLight™ Plus BioAssay.
Cell Culture and Cell Analysis using the Real Architecture for 3D Tissue (RAFT™) Culture System
1TAP Biosystems, Royston, UK;2Lonza Cologne GmbH, Cologne, Germany; 3Lonza Walkersville Inc., Walkersville, MD, USA

This poster explains how standard analysis techniques, like fluorescence microscopy, can be applied easily to RAFT™ 3D Cell Cultures.
Application Notes
Monitoring Saccharomyces Cerevisiea Growth with Bright Field Microscopy in Real Time
BioTek Instruments, Inc.

Strains of the yeast Saccharomyces cerevisiea serve critical roles in the production of many different products including food staples, medicines, and biofuels. The ability to obtain the desired product reliably and repeatedly requires careful monitoring of not only the input materials, but the growth of the yeast strain during the process.
Multipette®/ Repeater® M4 Allows for Fast, Precise and Sterile Liquid Transfer in Cell Culture
Eppendorf AG

This application note demonstrates the huge advantages offered by Multipette M4 in terms of accuracy and precision needed for many cell culture applications.
Aseptic Identification of Polysorbates using Handheld Raman
Rigaku Raman Technologies

As polysorbates are reactive to light and air, packaging is usually in thick, dark, amber bottles. This makes the identification process more difficult, as opening the container could compromise the sterility and quality of these materials. Handheld Raman is capable of identifying these materials without any sampling or preparation.
Cell Culture Media Identification In Biopharmaceuticals With Handheld Raman
Rigaku Raman Technologies

This application note discusses the identification of cell culture media using Raman analysis.
Performing ADCC Reporter Bioassay with an Environmentally Controlled Microplate Reader
Tecan Group Ltd.

The Promega ADCC Reporter Bioassay provides effector and target cells in frozen, thaw-and-use format, removing the need for isolating or culturing cells in order to perform the assay. Reporter induction requires a six-hour to overnight incubation in a standard cell culture incubator. The CO2 and temperature control features of the Tecan Infinite® 200 PRO with GCM allow the assay induction to be performed without the requirement for a cell culture incubator.
3D Cell Culture: mimicking real tissue
Tecan Group Ltd.

Technological advances in the last couple of years have enabled 3D scaffolds to be engineered that offer a real proxy for the in vivo environment which can be easily and routinely used in a cell culture laboratory. Alvetex Scaffold enables cells to develop natural, in vivo-like intercellular interactions, providing an ideal environment for real three-dimensional growth and nutrient exchange that is comparable to intra-capillary exchange in living tissues.
3D cell culture: a realistic and reproducible collagen-based environment
Tecan Group

Researchers are increasingly turning to 3D cell culture techniques, due to the improved physiological relevance of the cellular environment. 3D cell cultures offer a more in vivo-like response to stimuli more closely resembling the behavior of animal models or the human body.
Optimized cell culture
Tecan Group Ltd.

Culturing of various stable cell lines, including bacterial, yeast, hybridoma, insect and mammalian lines, and stem cells, is an important task in the pharmaceutical industry. The cells need to be maintained and expanded for use in cell-based assays, such as siRNA screening, ADME and high content screening, as well as for clone selection.
Determination of microbial cell viability
Tecan Group Ltd.

The BacTiter-Glo Microbial Cell Viability Assay is a luminescence-based assay for the determination of the viability of microbial cells, using quantification of ATP as an indicator of metabolically active, viable cells. The assay protocol comprises the addition of the BacTiter-Glo reagent directly to the sample, and subsequent measurement of luminescence; cell washing, removal of culture medium, and multiple pipetting steps are not required.
Stem cell research: Creating blood for high throughput screening
Tecan Group Ltd.

Many different research fields, including investigation of early human development processes, disease research, drug discovery and cellular therapy applications, require appropriate in vitro models which can accurately mimic in vivo processes to provide biologically relevant results. Pluripotent stem cells (PSCs) offer a powerful system for creating in vitro models to address these questions.
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