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Wednesday, October 22, 2014
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Featured Products
Featured ProjectTecan’s Infinite® 200 PRO microplate reader with Gas Control Module* (GCM™) offers in-reader incubation
Tecan’s Infinite® 200 PRO microplate reader with Gas Control Module* (GCM™) offers in-reader incubation
Featured ProjectEASYstrainer™ – just EASY
EASYstrainer™ – just EASY

Webcasts
iPS-based Automated Drug Screening: From Human iPS to Midbrain Dopaminergic NeuronsiPS-based Automated Drug Screening: From Human iPS to Midbrain Dopaminergic Neurons
Jan Bruder, Max Planck Institute Münster, speaking at Advances in Automation & Robotics 2014
3D Organotypic Liver Cultures and their Application in Predictive Toxicology3D Organotypic Liver Cultures and their Application in Predictive Toxicology
Fozia Noor, Saarland University, speaking at Advances in cellular Assays & Cell Culture 2014

Market Reports
Immunoassay Auto Trends 2014
HTStec

This report comprehensively documents current practices and preferences in immunoassays and to understand future user requirements, particularly with respect to immunoassay automation.
Cell-Based Label-Free Detection Trends 2014
HTStec Limited

This market report summarizes the results of HTStec’s 4th global web-based benchmarking survey on cell-based label-free (LF) detection carried out in July and August 2014. The study also examined in detail the current market landscape, application areas and interest in purchasing new products/systems.
Biologics
RI Technologies

This report on Biologics gives a market insight into products types including Monoclonal Antibodies, Blood & Blood Products, Vaccines, Insulin and Other, for both human and animal consumption.
Multi-Mode Microplate Reader Trends 2013
HTStec

This market report summarizes the results of HTStec’s 4th industry-wide global web-based benchmarking survey on multi-mode microplate readers carried out in September 2013.
3D Cell Culture Trends 2013
HTStec

This market report summarizes the results of HTStec’s 3rd industry-wide global web-based benchmarking survey on three dimensional (3D) cell culture technologies carried out in July 2013.
Cell Culture - Rising Demand for Biologics and Growing Visibility of Approval Pathway for Biosimilars will Create Growth Opportunities
Global Data

The report analyzes the role of biopharmaceutical products that basically provide enhancement to media, sera, reagents and cell culture market. Major manufacturing technologies involved in the cell culture technology have been detailed in the report. Furthermore, the report provides competitive benchmarking for the leading players that shape the global media, sera, reagent and overall cell culture market.
Cell Migration Assays Trends
HTStec

This market report summarizes the results of HTStec’s global web-based benchmarking survey on cell migration, motility or invasion assays carried out in July 2012.
Scientific News
Lab-developed Intestinal Organoids form Mature Human Tissue in Mice
Study produces unprecedented model to study intestinal diseases.
Culture System Replicates Course Of Alzheimer’s Disease
Three-dimensional system should significantly reduce time and costs of drug development.
Researchers Describe New Method for Cell Line Authentication
SNP profiling, an effective method to detect misidentification, contamination, and chromosomal abnormalities in mouse cell lines.
Giant Leap Against Diabetes
Ability to produce embryonic stem cells will allow researchers to push faster toward cure.
Vital New Insight into How we Produce New Brain Cells
Researchers have identified a key mechanism in the birth of new brain cells, with implications for treating brain injury and diseases.
How Fluid Flow Influences Neuron Growth
A University of Texas at Arlington team exploring how neuron growth can be controlled in the lab and, possibly, in the human body has published a new paper in Nature Scientific Reports on how fluid flow could play a significant role.
A New Molecule Allows for an Increase in Stem Cell Transplants
A clinical study using UM171 and a new type of bioreactor developed for stem culture will be initiated in December 2014.
Scientists Discover Neurochemical Imbalance in Schizophrenia
Researchers discovers that neurons from schizophrenia patients secrete higher amounts of three neurotransmitters.
Diabetes Researchers Find Faster Way to Create Insulin-producing Cells
A new protocol can turn stem cells into reliable, insulin-producing cells in about six weeks.
New Defence Mechanism Against Viruses Discovered
Mechanism may represent one of the oldest defence mechanisms against viruses in evolutionary history.
Business News
Anatrace, Microlytic Partnership to Enable Membrane Protein Research
Partnership will develop and commercialize Analytic, a new brand of products targeted to the membrane protein research community.
InSphero Wins ACES Award as Top European Academic Enterprise in Life Sciences
Second honor in three weeks continues momentum for leading supplier of 3D microtissues for in vitro toxicity and efficacy testing.
BioSilta and Sigma-Aldrich Sign Global Distribution Agreement
EnPresso growth systems now available to research scientists through Sigma-Aldrich.
MIMETAS Raises 5.2 Million Dollar For Organ-on-a-Chip
MIMETAS B.V. has raised 5.2 million Dollar to fund its expansion as a global leader in organ-on-a-chip technology.
Merck and TAP Biosystems Finalists for “Best Collaboration Award”
Recognizing ambr250 as a breakthrough technology for process development.
InSphero Named #1 Swiss Startup for 2014
Leading supplier of 3D microtissues for in vitro toxicity and efficacy testing earns top honors in annual Swiss startup competition.
TAP and Gallus Co-host Free Webinar
Explaining the benefits of using ambr15 microbioreactors for DoE.
Yale University and Leica Microsystems Partner
Yale welcomes scientists to participate in core facility opening and super-resolution workshops October 20 through 31, 2014.
Avacta Life Sciences Takes on the $55BN Antibody Market with Launch of Affimers
Alternative to antibodies launches offering multiple advantages to research teams.
STEMCELL Technologies Wins Support from Western Innovation (WINN) Initiative
Company has received $949,500 for commercialization of 3D cell culture platform.

Events
Translational Regenerative Med
21 Oct 2014
Translational Regenerative Med ...
More Information
Advances in Stem Cell Research
12 Nov 2014
Advances in Stem Cell Research ...
More Information
3rd Annual Cell Culture & Biop
17 Nov 2014
3rd Annual Cell Culture & Biop ...
More Information
FAST: Functional Analysis & Sc
17 Nov 2014
FAST: Functional Analysis & Sc ...
More Information

ePosters
High Titre BacMam viruses improve transduction efficiency of mammalian cells
Oxford Expression Technologies Ltd, Oxford Brookes University

We present a method for producing high titre BacMam virus vectors for the efficient transduction of mammalian cells.
Identification and Characterisation of Novel Positive Allosteric Modulators of the Galanin 2 Receptor
MRCT

An HTS against GalR2 was carried out to find novel PAMs to treat neuropathic pain.
The Longest Way Round is the Shortest Way Home: HTS Assay Development for Complex Multi-domain Protein Kinases
Axxam

This study strongly supports the possibility to overcome the major bottlenecks in the production of long kinase recombinant forms, in order to perform drug discovery programmes with proteins more closely preserving the structural and functional properties of the native enzymes.
Automated Fluorescence Detection and Imaging of RNA Species in Live Cells
1BioTek Instruments, Inc., Winooski, Vermont • 2EMD Millipore Temecula, California

This poster describes the detection and quantification of RNA species in Live cells through automated imaging.
Validation of a Novel Tumoroid-Based Cell Culture Model to Perform 3D in vitro Cell Signaling Analyses
1BioTek Instruments, Inc., Winooski, Vermont, USA • 2TAP Biosystems, Royston, Hertfordshire, UK 3Cisbio US, Inc., Bedford, Massachusetts, USA

Here we demonstrate an in vitro HTRF® microplate assay that can quantify total, as well as phosphorylated elF4E.
Validation of a 3-Dimensional Human Liver Microtissue Model for Long-term Hepatotoxicity Studies
1BioTek Instruments, 2InSphero, Inc. 3Bioreclamation I VT, 4 Promega Corporation

Non-steroidal anti-inflammatory drugs (NSAIDs) are a class of drug commonly used as analgesics and antipyretics, as well as for management of rheumatological disorders. They are one of the most highly prescribed drug families around the world, and consequently, along with antimicrobial agents, are the most frequent causes of druginduced liver injury (DILI) (Bjornsson et al., 2010).
Liposomes and Nanoparticles as Delivery Vehicles for the Treatment of Lung Diseases
1Clemson University, Clemson, SC, 2Ralph H. Johnson VA Medical Center, Charleston, SC

Targeted nanoparticle-based drug delivery to the lungs is an emerging area of interest for both scientists and medical workers because it allows better drug retention in the lungs and can provide prolonged drug release. In our study we investigated the potential of using liposomes and polymeric nanoparticles surface modified with a specific antibody as drug carriers for targeted drug delivery to the lungs.
Side by Side: An Evaluation of 2D vs. 3D Cell Culture for High Throughput Screening in Drug Discovery
1 High Throughput Screening, Discovery Sciences, AstraZeneca Alderley Park. 2 University of York, Biology Department. 3 Chemistry, iMED Oncology, AstraZeneca Alderley Park.

•3D cell culture has the potential to provide a more physiologically relevant model compared to standard tissue culture plastic.
•From a screening perspective the technology offers the possibility of more predictive drug responses but has an increased cost.
•The question: is it possible and, more importantly, is it worthwhile moving towards screening in High Throughput using a 3D model?
Progressing 3D Spheroid Analysis into a HTS Drug Discovery Method
Nexcelom LLC

3D Tumorspheroid models for improved predictivity in cancer drug discovery.
Gut Microbial Metabolites and Hepatic Xenobiotic Metabolism: A High Throughput Screening Approach
University of Reading, AstraZeneca

This poster highlights the combination of metabonomics and high throughput screening by the identification of gut microbial metabolites and a screening assay designed to determine their cytotoxicity to liver-like cell cultures.
Application Notes
Performing ADCC Reporter Bioassay with an Environmentally Controlled Microplate Reader
Tecan Group Ltd.

The Promega ADCC Reporter Bioassay provides effector and target cells in frozen, thaw-and-use format, removing the need for isolating or culturing cells in order to perform the assay. Reporter induction requires a six-hour to overnight incubation in a standard cell culture incubator. The CO2 and temperature control features of the Tecan Infinite® 200 PRO with GCM allow the assay induction to be performed without the requirement for a cell culture incubator.
3D Cell Culture: mimicking real tissue
Tecan Group Ltd.

Technological advances in the last couple of years have enabled 3D scaffolds to be engineered that offer a real proxy for the in vivo environment which can be easily and routinely used in a cell culture laboratory. Alvetex Scaffold enables cells to develop natural, in vivo-like intercellular interactions, providing an ideal environment for real three-dimensional growth and nutrient exchange that is comparable to intra-capillary exchange in living tissues.
3D cell culture: a realistic and reproducible collagen-based environment
Tecan Group

Researchers are increasingly turning to 3D cell culture techniques, due to the improved physiological relevance of the cellular environment. 3D cell cultures offer a more in vivo-like response to stimuli more closely resembling the behavior of animal models or the human body.
Optimized cell culture
Tecan Group Ltd.

Culturing of various stable cell lines, including bacterial, yeast, hybridoma, insect and mammalian lines, and stem cells, is an important task in the pharmaceutical industry. The cells need to be maintained and expanded for use in cell-based assays, such as siRNA screening, ADME and high content screening, as well as for clone selection.
Determination of microbial cell viability
Tecan Group Ltd.

The BacTiter-Glo Microbial Cell Viability Assay is a luminescence-based assay for the determination of the viability of microbial cells, using quantification of ATP as an indicator of metabolically active, viable cells. The assay protocol comprises the addition of the BacTiter-Glo reagent directly to the sample, and subsequent measurement of luminescence; cell washing, removal of culture medium, and multiple pipetting steps are not required.
Stem cell research: Creating blood for high throughput screening
Tecan Group Ltd.

Many different research fields, including investigation of early human development processes, disease research, drug discovery and cellular therapy applications, require appropriate in vitro models which can accurately mimic in vivo processes to provide biologically relevant results. Pluripotent stem cells (PSCs) offer a powerful system for creating in vitro models to address these questions.
Live Cell Biosensor Assay Used To Interrogate GPCRs - Comparative Analysis of Biosensor Variants
BioTek Instruments

This application note compares the performance of the highest and lowest affinity variants using automated methods and the highly-sensitive bottom reading capabilities of an HTS microplate reader. Sensitive detection of the 22F basal signal allows for use of this variant under a broad range of conditions, providing an assay with both superior dynamic range and sensitivity.
Direct Visualisation, Sizing and Counting of Aggregation in Proteins
NanoSight Ltd

Characterising the state of aggregation in proteins is of paramount importance when trying to understand biopharmaceutical product stability and efficacy. Product quality, both in terms of biological activity and immunogenicity can be highly influenced by the state of protein aggregation.
Automated Growth Monitoring of Microaerophilic Organisms
Tecan Group - Products

Walkaway growth monitoring of Helicobacter pylori in the Infinite® 200 PRO reader with Gas Control Module (GCM™).
Rapid Screening of a Cell-based Assay for GLP-1 Receptor Using a Natural Product Library
BioTek Instruments

Here we demonstrate the detection of GLP-1R binding through the incorporation of a homogeneous cell-based binding assay using HTRF Tag-lite® technology.
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