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Featured Products
Featured ProjectRAFT™ 3D Cell Culture System for Tissue Modeling
RAFT™ 3D Cell Culture System for Tissue Modeling
Featured ProjectMaxCyte® STX™ Scalable Transfection System
MaxCyte® STX™ Scalable Transfection System

Webcasts
Rapid and Sensitive Identification of Host Cell Proteins with SWATH® LC-MS AcquisitionRapid and Sensitive Identification of Host Cell Proteins with SWATH® LC-MS Acquisition
Join Eric Johansen PhD, Senior Scientist at Stemcentrx, to learn how to dramatically increase the speed of acquisition of HCP data.
Lab-on-Chip Platform Technologies for Point-of-Care Testing and Cell CulturingLab-on-Chip Platform Technologies for Point-of-Care Testing and Cell Culturing
This presentation focuses on recent advances in platform technologies developed at Micronit for applications within point-of-care diagnostics and cell culturing. A few examples developed within research projects are presented to illustrate the applicability of the developed technologies.

Market Reports
3D Spheroid Culture Trends 2015
HTStec Limited

This market report summarizes the results of HTStec’s industry-wide global web-based benchmarking survey on three dimensional (3D) spheroid culture carried out in May 2015.
Immunoassay Auto Trends 2014
HTStec

This report comprehensively documents current practices and preferences in immunoassays and to understand future user requirements, particularly with respect to immunoassay automation.
Cell-Based Label-Free Detection Trends 2014
HTStec Limited

This market report summarizes the results of HTStec’s 4th global web-based benchmarking survey on cell-based label-free (LF) detection carried out in July and August 2014. The study also examined in detail the current market landscape, application areas and interest in purchasing new products/systems.
Scientific News
Featured Article
The Rise of 3D Cell Culture and in vitro Model Systems for Drug Discovery and Toxicology
An overview of the current technology and the challenges and benefits over 2D cell culture models plus some of the latest advances relating to human health research.

A Boost for Regenerative Medicine
Growing tissues and organs in the lab for transplantation into patients could become easier after scientists discovered an effective way to produce three-dimensional networks of blood vessels, vital for tissue survival yet a current stumbling block in regenerative medicine.
Bio-Mimicry Method For Preparing & Labeling Stem Cells Developed
Method allows researchers to prepare mesenchymal stem cells and monitor them using MRI.
Stem Cell Advance Could Be Key Step Toward Treating Deadly Blood Diseases
UCLA scientists get closer to creating blood stem cells in the lab.
Harnessing Engineered Slippery Surfaces For Tissue Repair
A new method could facilitate the transfer of intact regenerating cell sheets from the culture dish to damaged tissues in patients.
Brazilian Zika Virus Strain Causes Birth Defects in Experimental Models
First direct experimental proof of causal effect, researchers say.
Gut Model HuMiX Works Like the Real Thing
Developed by scientists at the Luxembourg Centre for Systems Biology, the “Human Microbial Cross-talk” model is representative of the actual conditions and processes that occur within our intestines.
'Kidney on a Chip' Facilitates Safer Drug Dosing
University of Michigan researchers have used a "kidney on a chip" device to mimic the flow of medication through human kidneys and measure its effect on kidney cells.
New Method Allows First Look At Embryo Implantation
Researchers at The Rockefeller University develop a method that shows the molecular and cellular processes that occur up to day 14 after fertilization.
Detecting Nano Amounts In Environmental Samples
The NanoUmwelt project is developing a technique that can detect nanomaterials in a variety of environmental samples.
Business News
Evercyte, VTU Technology Collaborate
Launch of first joint product – VEGF 165 for cell culture applications.
Irvine Scientific Receives FDA 510(k) Clearance
Company has announced that it has received FDA 510(k) clearance for expanded use of Vit Kit® vitrification media to include human oocytes.
Eppendorf Continues Successful Development
Group sales of €629.1 million (18.5% growth in sales).
GE Healthcare Expands European Capabilities
Expansion will see large volume sterile liquid handling capacity doubled.
Global 3D Cell Culture Market: Increasing Investment Acts as Growth Driver
The Europe 3D cell culture market was valued at US$ 234.4 Mn in 2014 and is expected to account for US$ 1,067.8 Mn by 2020 at a CAGR of 28.7%.
Growing Skin in the Lab
Using reprogrammed iPS cells, scientists have successfully grown complex skin tissue—complete with hair follicles and sebaceous glands—in the laboratory.
Oncology, Drug Development and Toxicology to Fuel Rise of 3D Cell Culture
On the basis of the end user, biotechnology and biopharmaceutical industries dominate the 3D cell culture market.
Plasticell and Sphere Fluidics Sign Research Collaboration
Company has announced collaboration with Sphere Fluidics to develop gene transduction technology leading to safer and more efficient gene therapies.
3D Medical EXPO, 3D Bioprinting Conference Wrap Up
The 3D Bioprinting Conference concluded on January 26, 2016 in Maastricht, Netherlands. It was part of the 3rd edition of the 3D Medical Expo, which included a two day exhibition and three more conferences.
URMC Partners with Indivumed
The University of Rochester Medical Center announced it’s collaborating with Indivumed, a Germany-based company, to establish a bank of human tissues and tumor samples that are expertly preserved and stored for use in cancer research.

Events
Bioimaging: From Cells To Mole
14 Jun 2016
Bioimaging: From Cells To Mole ...
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Organ-on-a-Chip World Congress
07 Jul 2016
Organ-on-a-Chip World Congress ...
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The Antibody-based Bio-therape
13 Jul 2016
The Antibody-based Bio-therape ...
More Information
Bioprinting & 3D Printing in t
21 Jul 2016
Bioprinting & 3D Printing in t ...
More Information

ePosters
Phenotypic Cell-Based Screening of a High Content Imaging Cell Health Assay using the IN Cell Analyzer 2000
MRC Technology

A High Content Imaging Cell Health assay has been established in-house for phenotypic High Content Screening using the IN Cell Analyzer 2000 and Genedata Screener analysis software. We describe the assay development and initial screening of the FDA set in HepG2 cells for validation of the assay.
Analyzing Cell Viability in 3D Tissue Models with the ViaLight™ Plus BioAssay
1Lonza Cologne GmbH, Cologne, Germany; 2Lonza Walkersville Inc., Walkersville, MD, USA; 3Lonza Rockland Inc., Rockland, ME, USA

This poster explains how to measure cell viability easily in 3D cell cultures using the ViaLight™ Plus BioAssay.
Cell Culture and Cell Analysis using the Real Architecture for 3D Tissue (RAFT™) Culture System
1TAP Biosystems, Royston, UK;2Lonza Cologne GmbH, Cologne, Germany; 3Lonza Walkersville Inc., Walkersville, MD, USA

This poster explains how standard analysis techniques, like fluorescence microscopy, can be applied easily to RAFT™ 3D Cell Cultures.
Comparison of Normal and Asthmatic Bronchial Epithelial Cells and Smooth Muscle Cells in Monolayer and RAFT™ 3D Cell Culture System
1Lonza Walkersville Inc. Walkersville MD USA, 2Lonza Cologne GmbH, Cologne, Germany

The RAFT™ 3D cell culture system provides a valuable tool to investigate different cell types singularly or in co-cultures in an in-vivo like collagen based microenvironment.
Pharmacological Responses in Cultured Human iPSC-Derived Cortical Neurons Using Multi-Electrode Array
(1) Department of Electronics and Intelligent Systems, Tohoku Institute of Technology, Sendai, Miyagi, Japan; (2) Japan Society for the Promotion of Science, Tokyo, Japan; (3) Axol Bioscience Ltd., Ca

The functional network of human induced pluripotent stem cell (hiPSC)-derived neurons is a potentially powerful in vitro model for evaluating disease mechanisms and drug responses. However, the culture time required for the full functional maturation of individual neurons and networks is uncertain. We investigated the development of spontaneous electrophysiological activity and pharmacological responses for over 1 year in culture using multi-electrode arrays (MEAs).
Translational Research of oral Neural Crest-Derived Stem Cells (oNCSCs) in Regenerative Dentistry
1Stavropol State Medical University, Russian Federation, 2Praxisteam Hasslinghausen, Germany, 3Implantology Center Kassel, Germany, 4Witten/ Herdecke University, Germany, 5University of Reading, UK, 6

In this review poster, we summarize current knowledge on the oral neural crest-derived stem cell populations (oNCSCs) and discuss their potential in regenerative periodontology as a part of regenerative dentistry.
Design considerations for highly specific and efficient synthetic crRNA molecules
GE Healthcare Dharmacon, Inc.

An overview of our rational design algorithm for picking highly functional crRNA sequences in combination with comprehensive specificity analysis.
CellTiter-Glo® 2.0: A Novel Luminescent Cell Viability Assay with Greatly Enhanced Storage Stability
Promega Corporation

Here we report on the attributes of a novel ATP detection reagent for cell viability with all of the assay performance of the previous CellTiter-Glo® Reagent, but now with markedly enhanced stability as a single component in a liquid format. These new features provide for much greater ease-of-use in that storage of the reagent at 4°C eliminates the requirement for reagent thawing and minimizes temperature equilibration time.
Design and Validation of Bioluminescent Assays for 3D Cell Culture Models Poster
Promega Corporation

Cells cultured in 3D model systems often acquire relatively large in vivo-like structures compared to the thickness of a 2D monolayer of cells grown on standard plastic plates.
Testing a Novel Real Time Cell Viability Assay
Promega Corporation

Recently developed assay technologies make it possible to use multi-well plate readers to measure the number of live or dead cells in culture in real time over a period of days. Live cells are measured in real time by adding a reagent containing a shrimp-derived luciferase and a pro-substrate directly to the culture medium. Only viable cells can convert the pro-substrate into a luciferase substrate and generate light.
Application Notes
Multipette®/ Repeater® M4 Allows for Fast, Precise and Sterile Liquid Transfer in Cell Culture
Eppendorf AG

This application note demonstrates the huge advantages offered by Multipette M4 in terms of accuracy and precision needed for many cell culture applications.
Aseptic Identification of Polysorbates using Handheld Raman
Rigaku Raman Technologies

As polysorbates are reactive to light and air, packaging is usually in thick, dark, amber bottles. This makes the identification process more difficult, as opening the container could compromise the sterility and quality of these materials. Handheld Raman is capable of identifying these materials without any sampling or preparation.
Cell Culture Media Identification In Biopharmaceuticals With Handheld Raman
Rigaku Raman Technologies

This application note discusses the identification of cell culture media using Raman analysis.
Performing ADCC Reporter Bioassay with an Environmentally Controlled Microplate Reader
Tecan Group Ltd.

The Promega ADCC Reporter Bioassay provides effector and target cells in frozen, thaw-and-use format, removing the need for isolating or culturing cells in order to perform the assay. Reporter induction requires a six-hour to overnight incubation in a standard cell culture incubator. The CO2 and temperature control features of the Tecan Infinite® 200 PRO with GCM allow the assay induction to be performed without the requirement for a cell culture incubator.
3D Cell Culture: mimicking real tissue
Tecan Group Ltd.

Technological advances in the last couple of years have enabled 3D scaffolds to be engineered that offer a real proxy for the in vivo environment which can be easily and routinely used in a cell culture laboratory. Alvetex Scaffold enables cells to develop natural, in vivo-like intercellular interactions, providing an ideal environment for real three-dimensional growth and nutrient exchange that is comparable to intra-capillary exchange in living tissues.
3D cell culture: a realistic and reproducible collagen-based environment
Tecan Group

Researchers are increasingly turning to 3D cell culture techniques, due to the improved physiological relevance of the cellular environment. 3D cell cultures offer a more in vivo-like response to stimuli more closely resembling the behavior of animal models or the human body.
Optimized cell culture
Tecan Group Ltd.

Culturing of various stable cell lines, including bacterial, yeast, hybridoma, insect and mammalian lines, and stem cells, is an important task in the pharmaceutical industry. The cells need to be maintained and expanded for use in cell-based assays, such as siRNA screening, ADME and high content screening, as well as for clone selection.
Determination of microbial cell viability
Tecan Group Ltd.

The BacTiter-Glo Microbial Cell Viability Assay is a luminescence-based assay for the determination of the viability of microbial cells, using quantification of ATP as an indicator of metabolically active, viable cells. The assay protocol comprises the addition of the BacTiter-Glo reagent directly to the sample, and subsequent measurement of luminescence; cell washing, removal of culture medium, and multiple pipetting steps are not required.
Stem cell research: Creating blood for high throughput screening
Tecan Group Ltd.

Many different research fields, including investigation of early human development processes, disease research, drug discovery and cellular therapy applications, require appropriate in vitro models which can accurately mimic in vivo processes to provide biologically relevant results. Pluripotent stem cells (PSCs) offer a powerful system for creating in vitro models to address these questions.
Live Cell Biosensor Assay Used To Interrogate GPCRs - Comparative Analysis of Biosensor Variants
BioTek Instruments

This application note compares the performance of the highest and lowest affinity variants using automated methods and the highly-sensitive bottom reading capabilities of an HTS microplate reader. Sensitive detection of the 22F basal signal allows for use of this variant under a broad range of conditions, providing an assay with both superior dynamic range and sensitivity.
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