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Featured ProjectHyStem® Hydrogels UV QuickSet Kit
HyStem® Hydrogels UV QuickSet Kit
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Standard FLEXCULTURE™ Rocking Bioreactor

Webcasts
Modifying Cell Lines for Target Discovery and ValidationModifying Cell Lines for Target Discovery and Validation
In this 30-minute seminar, we will discuss the process of genome editing, address factors affecting the design of a genome editing project, and explore the application of modified cell lines for target discovery and validation.
Accelerate Your Research With Tecan´s New Multimode Microplate Reader Platform!Accelerate Your Research With Tecan´s New Multimode Microplate Reader Platform!
In this webinar, solutions will be presented for fast DNA quantification, single cell counting and viability analysis, and automating long-term live kinetic cell assays.

Market Reports
Immunoassay Auto Trends 2014
HTStec

This report comprehensively documents current practices and preferences in immunoassays and to understand future user requirements, particularly with respect to immunoassay automation.
Cell-Based Label-Free Detection Trends 2014
HTStec Limited

This market report summarizes the results of HTStec’s 4th global web-based benchmarking survey on cell-based label-free (LF) detection carried out in July and August 2014. The study also examined in detail the current market landscape, application areas and interest in purchasing new products/systems.
Biologics
RI Technologies

This report on Biologics gives a market insight into products types including Monoclonal Antibodies, Blood & Blood Products, Vaccines, Insulin and Other, for both human and animal consumption.
Multi-Mode Microplate Reader Trends 2013
HTStec

This market report summarizes the results of HTStec’s 4th industry-wide global web-based benchmarking survey on multi-mode microplate readers carried out in September 2013.
3D Cell Culture Trends 2013
HTStec

This market report summarizes the results of HTStec’s 3rd industry-wide global web-based benchmarking survey on three dimensional (3D) cell culture technologies carried out in July 2013.
Cell Culture - Rising Demand for Biologics and Growing Visibility of Approval Pathway for Biosimilars will Create Growth Opportunities
Global Data

The report analyzes the role of biopharmaceutical products that basically provide enhancement to media, sera, reagents and cell culture market. Major manufacturing technologies involved in the cell culture technology have been detailed in the report. Furthermore, the report provides competitive benchmarking for the leading players that shape the global media, sera, reagent and overall cell culture market.
Cell Migration Assays Trends
HTStec

This market report summarizes the results of HTStec’s global web-based benchmarking survey on cell migration, motility or invasion assays carried out in July 2012.
Scientific News
A Single-Cell Breakthrough
UNC School of Medicine scientist Scott Magness and collaborators use their newly developed technology to dissect properties of single stem cells. The advancement will allow researchers to study gastrointestinal disorders and cancers like never before.
‘Mini-Lungs’ Grown To Aid The Study Of Cystic Fibrosis
'Mini-lungs’ have been created using stem cells derived from skin cells of patients with cystic fibrosis.
Designing A Better Way To Study Stomach Flu
Texas Medical Center team aims to improve research of gastrointestinal disease.
Hearts On A Chip To Aid Drug Screening
UC Berkeley bioengineers have developed a heart-on-a-chip which can be used for drug safety screening.
Using Human Stem Cells To Identify Dangerous Side Effects Of Drugs
Scientists have developed a test that uses cells from a single donor's blood to predict whether a new drug will cause a severe reaction in humans.
Preventing Childhood Illnesses Could Stop Later Life-Threatening Infections for CF Patients
Aim of the study is to establish new methods to reduce the number of animals used in respiratory studies.
3D-Printed Guides Can Help Restore Function In Damaged Nerves
Scientists at the University of Sheffield have succeeded in using a 3D printed guide to help nerves damaged in traumatic incidents repair themselves.
Safer Methods for Stem Cell Culturing
New study from TSRI shows that certain stem cell culture methods are associated with increased DNA mutations.
3-D Engineered Bone Marrow Makes Functioning Platelets
Scalable model supports patient-specific treatments, advanced study of blood disorders.
New Screening Tool Could Speed Development Of Ovarian Cancer Drugs
Researchers have built a 3D cell culture system which can help identify small molecules that could be used to treat ovarian cancer.
Business News
INTEGRA Biosciences Opens German Subsidiary
Company has also launched a new comprehensively featured German language website.
Lonza and CytoMate Collaboration on CytoSMART™ Technology
Agreement for global distribution of the new technology which is designed for easy, affordable live cell imaging.
Horizon, Centre for Process Innovation, & University of Manchester Awarded £1.67M Innovate UK Funding
Consortium to receive funding to develop enhanced biomanufacturing platforms based on the Company’s CHO cell line and gene editing platform.
INFORS HT Celebrates its 50th Anniversary
As well as celebrating its own achievements, INFORS HT will be giving something back to society in 2015.
Organovo Holdings Reports Third Quarter Results
Company expects commercial revenue from post-launch activity to be recognized ending June 30, 2015.
AstraZeneca Forms CRISPR Collaborations
AstraZeneca announces collaborations to use CRISPR technology for genome editing across its drug discovery platform.
SAFC’s Dry Powder Media Manufacturing Facility in Irvine, Scotland
Officially opens following extensive validation process.
3D Liver Spheroid Model in Use by MIP-DILI
European consortium will use 3D liver tissues to better predict drug-induced liver injury.
First Stem-Cell Therapy Recommended For Approval In EU
New treatment for rare condition caused by burns to the eye.
OHSU and FEI Expand Living Lab with New Equipment for Cancer Research
Installation is part of an expansion of the OHSU/FEI Living Lab for Cell Biology collaboration.

Events
4th Cell Line Engineering & De
19 May 2015
4th Cell Line Engineering & De ...
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Cell Culture World Congress US
27 May 2015
Cell Culture World Congress US ...
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Downstream Processing USA 2015
27 May 2015
Downstream Processing USA 2015 ...
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Bioprocess International China
12 Aug 2015
Bioprocess International China ...
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ePosters
Development of a novel xeno-free medium for feeder-free culture of human stem cells
ReproCELL, Inc.

A new xeno-free medium (ReproXFTM) has been developed for feeder-free culture of stem cells.
Novel culture medium using a small-molecule agonist of thrombopoetin receptor
1ReproCELL, Inc. & 2Nissan Chemical Industries

ReproHSC medium for the culture of hematopoietic stem cells. facilitates the culture and expansion of CD34+/CD38- cells that retain their HSC properties
A novel multi-organ microfluidic chip: on the way to the complexity of a living organism
Hemule GmbH

A novel physiologically relevant multi-organ chip is developed capable of culturing up to six different organotypic models integrated into a single microfluidic circuit.
Continuous Collection of Stem Cells from a Human Placenta Perfusion Co-Culture
FiberCell Systems Inc. & Hemacell Perfusion Inc.

The potential for human placenta-derived cells to produce stem cells in a hollow fiber bioreactor co-culture system was investigated.
Identification of microRNA targets using microRNA modulation techniques and gene expression arrays


By examining the overlap of messages down-regulated by miRNA mimics and up-regulated by miRNA inhibitors, we robustly identify miRNA-regulated messages, many of which have canonical seed matches and some which are not identied by standard target prediction programs.
Cell-Mediated Cytotoxicity Assay by High-Throughput Direct Cell Counting in Microplates using Fluorescence-Based Image Cytometry
Nexcelom Bioscience LLC,The University of Texas MD Anderson Cancer Center.

In this work, we demonstrate a novel high throughput cytotoxicity detection assay using the Celigo Imaging Cytometry method. Utilizing imaging cytometry, direct cell counting of live fluorescent target cells can be performed, which is a direct method for assessment of cytotoxicity.
Idebenone Inhibits Cell Proliferation by Blocking of ANO1/ TMEM16A Chloride Channel in Adenocarcinoma Cells
1Department of Integrated Omics for Biomedical Science, WCU Program of Graduate School, Yonsei University, Korea

Idebenone significantly reduced cell proliferation and induced apoptosis in PC-3, CFPAC-1, HT-29, T-84 and Calu-3 cells having CaCCs activities. These data suggest that idebenone, an ANO1/CaCC inhibitor, has potential for use in cancer therapy.
Non-disruptively Count and Quantify Fluorescent iPS Colonies During Secondary Reprogramming: 7 min per 6-well Plate, Dual-fluorescence Whole Well Imaging Cytometry
Nexcelom Bioscience; University of Edinburgh

Nexcelom’s Celigo imaging cytometer has been applied to provide automated, rapid assessment of iPS reprogramming.

Progressing 3D Spheroid Analysis into a HTS Drug Discovery Method
Nexcelom Bioscience, LLC

This study demonstrates that the cell culture assay for tumorsphere size was successfully adapted to 384-well plate for a 3D spheroid assay using U-bottom low attachment 384-well and the Celigo Automated Imager for image acquisition and analysis. Moreover, the utilization of an imager provided insight on sphere and cell fate after long term drug treatment.
Using An in‐vitro Human Airways Model to Study the Effects of e‐cigarettes on Bronchial Epithelial Cells
1 School of Engineering and Applied Science, Aston University, Birmingham UK; 2 School of Health and Life Sciences, Aston University, Birmingham UK

The prime aim of our study is to use in-vitro human airway models as an effective alternative to replace animals in traditional/E-cigarette research.
Application Notes
Aseptic Identification of Polysorbates using Handheld Raman
Rigaku Raman Technologies

As polysorbates are reactive to light and air, packaging is usually in thick, dark, amber bottles. This makes the identification process more difficult, as opening the container could compromise the sterility and quality of these materials. Handheld Raman is capable of identifying these materials without any sampling or preparation.
Cell Culture Media Identification In Biopharmaceuticals With Handheld Raman
Rigaku Raman Technologies

This application note discusses the identification of cell culture media using Raman analysis.
Performing ADCC Reporter Bioassay with an Environmentally Controlled Microplate Reader
Tecan Group Ltd.

The Promega ADCC Reporter Bioassay provides effector and target cells in frozen, thaw-and-use format, removing the need for isolating or culturing cells in order to perform the assay. Reporter induction requires a six-hour to overnight incubation in a standard cell culture incubator. The CO2 and temperature control features of the Tecan Infinite® 200 PRO with GCM allow the assay induction to be performed without the requirement for a cell culture incubator.
3D Cell Culture: mimicking real tissue
Tecan Group Ltd.

Technological advances in the last couple of years have enabled 3D scaffolds to be engineered that offer a real proxy for the in vivo environment which can be easily and routinely used in a cell culture laboratory. Alvetex Scaffold enables cells to develop natural, in vivo-like intercellular interactions, providing an ideal environment for real three-dimensional growth and nutrient exchange that is comparable to intra-capillary exchange in living tissues.
3D cell culture: a realistic and reproducible collagen-based environment
Tecan Group

Researchers are increasingly turning to 3D cell culture techniques, due to the improved physiological relevance of the cellular environment. 3D cell cultures offer a more in vivo-like response to stimuli more closely resembling the behavior of animal models or the human body.
Optimized cell culture
Tecan Group Ltd.

Culturing of various stable cell lines, including bacterial, yeast, hybridoma, insect and mammalian lines, and stem cells, is an important task in the pharmaceutical industry. The cells need to be maintained and expanded for use in cell-based assays, such as siRNA screening, ADME and high content screening, as well as for clone selection.
Determination of microbial cell viability
Tecan Group Ltd.

The BacTiter-Glo Microbial Cell Viability Assay is a luminescence-based assay for the determination of the viability of microbial cells, using quantification of ATP as an indicator of metabolically active, viable cells. The assay protocol comprises the addition of the BacTiter-Glo reagent directly to the sample, and subsequent measurement of luminescence; cell washing, removal of culture medium, and multiple pipetting steps are not required.
Stem cell research: Creating blood for high throughput screening
Tecan Group Ltd.

Many different research fields, including investigation of early human development processes, disease research, drug discovery and cellular therapy applications, require appropriate in vitro models which can accurately mimic in vivo processes to provide biologically relevant results. Pluripotent stem cells (PSCs) offer a powerful system for creating in vitro models to address these questions.
Live Cell Biosensor Assay Used To Interrogate GPCRs - Comparative Analysis of Biosensor Variants
BioTek Instruments

This application note compares the performance of the highest and lowest affinity variants using automated methods and the highly-sensitive bottom reading capabilities of an HTS microplate reader. Sensitive detection of the 22F basal signal allows for use of this variant under a broad range of conditions, providing an assay with both superior dynamic range and sensitivity.
Direct Visualisation, Sizing and Counting of Aggregation in Proteins
NanoSight Ltd

Characterising the state of aggregation in proteins is of paramount importance when trying to understand biopharmaceutical product stability and efficacy. Product quality, both in terms of biological activity and immunogenicity can be highly influenced by the state of protein aggregation.
SELECTBIO

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