Corporate Banner
Satellite Banner
Cell Culture
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

New Method Makes Culture of Complex Tissue Possible in any Lab

Published: Friday, February 10, 2012
Last Updated: Friday, February 10, 2012
Bookmark and Share
Scientists at the University of California, San Diego have developed a new method for making scaffolds for culturing tissue in three-dimensional arrangements that mimic those in the body.

This advance, published online in the journal Advanced Materials, allows the production of tissue culture scaffolds containing multiple structurally and chemically distinct layers using common laboratory reagents and materials.

According to the UC San Diego researchers, this process is more affordable and widely feasible than previous methods that required expensive equipment and expertise.

The new approach is remarkably simple: solutions of the components of each layer, including polymers, are mixed with varying concentrations of a common inert reagent to control density. The solutions are layered so that the difference in density segregates each solution, and then polymerized so that they form a gel. The structure of each layer can be altered by varying the concentration of polymers, and the discreteness of the transition between layers can be altered by allowing the solutions to diffuse.

Lead author Jerome Karpiak, graduate student in the UCSD Biomedical Sciences Program, said, “We’re excited about the relevance of this method to tissue engineering. Since it offers such straightforward spatial control over structure and composition of stratified tissue scaffolds, including cell type and density, this technology could help the field move much faster.” Tissues cultured in vitro to mimic those in the body can potentially provide an alternative to transplantation for injured or degenerated tissue.

“We believe this approach will vastly broaden the number of labs capable of culturing complex tissue,” said Adah Almutairi, PhD, assistant professor at the UCSD Skaggs School of Pharmacy and Pharmaceutical Sciences, the Department of Nanoengineering and the Materials Science and Engineering Program at the UCSD Jacobs School of Engineering.  “Because manipulation of structure and concentrations of signal molecules is much easier in this system than in intact organisms, it holds great potential to advance the study of development and disease.” For example, this method may offer a novel approach to study how surrounding molecules affect the growth of axons in neurodevelopmental disorders.

Additional researchers included Yogesh Ner, PhD. Research was funded in part by the National Institutes of Health Director’s New Innovator program and King Abdulaziz City of Science and Technology.


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,500+ scientific posters on ePosters
  • More than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.


Scientific News
Protein Related to Long Term Traumatic Brain Injury Complications Discovered
NIH-study shows protein found at higher levels in military members who have suffered multiple TBIs.
Women’s Immune System Genes Operate Differently from Men’s
A new technology reveals that immune system genes switch on and off differently in women and men, and the source of that variation is not primarily in the DNA.
DNA Damage Seen in Patients Undergoing CT Scanning
Along with the burgeoning use of advanced medical imaging tests over the past decade have come rising public health concerns about possible links between low-dose radiation and cancer.
Yeast Cells Use Signaling Pathway to Modify Their Genomes
Researchers at the Babraham Institute and Cambridge Systems Biology Centre, University of Cambridge have shown that yeast can modify their genomes to take advantage of an excess of calories in the environment and attain optimal growth.
New Material Forges the Way for 'Stem Cell Factories'
Researchers have discovered the first fully synthetic substrate with potential to grow billions of stem cells. The researchcould forge the way for the creation of 'stem cell factories' - the mass production of human embryonic (pluripotent) stem cells.
New Measurements Reveal Differences Between Stem Cells for Treating Retinal Degeneration
By growing two types of stem cells in a “3-D culture” and measuring their ability to produce retinal cells, a team lead by St. Jude Children’s Research Hospital researchers has found one cell type to be better at producing retinal cells.
Researchers Identify Critical Genes Responsible for Brain Tumor Growth
After generating new brain tumor models scientists have identified the role of a family of genes underlying tumor growth in a wide spectrum of high grade brain tumors.
Growing Spinal Disc Tissue
Scientists develop new method for growing spinal disc tissue in the lab for combating chronic back pain.
A New Path Towards a Universal Flu Vaccine
New research suggests it may be possible to harness a previously unknown mechanism within the immune system to create more effective and efficient vaccines against this ever-mutating virus.
Potential New Class of Cancer Drugs
Scientists have found a way to stop cancer cell growth by targeting the Warburg Effect, a trait of cancer cell metabolism that scientists have been eager to exploit.
SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!