Corporate Banner
Satellite Banner
Cell Culture
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Olympus Introduces 40x Silicone Oil Microscope Objective

Published: Friday, September 13, 2013
Last Updated: Friday, September 13, 2013
Bookmark and Share
Offers brighter, high-resolution live cell and time-lapse imaging.

Olympus has introduced a 40x silicone oil microscope objective, its latest innovation in silicone immersion microscope optics.

The new objective fills out the company’s full line of silicone oil optics, which already includes popular 30x and 60x objectives designed for live cell and time-lapse imaging.

Olympus silicone oil objectives can markedly improve optical performance for live cell confocal, widefield fluorescence, multiphoton, differential interference contrast (DIC) and other applications.

Both the new intermediate-magnification UPLSAPO 40x and the existing UPLSAPO 60x objective are designed specifically to work with the Olympus Zero Drift continuous autofocus system for extended time-lapse imaging.

In addition, all three of the objectives are compatible with protocols that use repeat-single-shot autofocus.

In contrast to water immersion objectives, silicone oil optics are useful in long-term imaging experiments where water evaporation is a pervasive issue.

While water immersion lenses are traditionally used to reduce refraction index mismatch, they are not practical for long-term, time-lapse imaging because of moisture loss and low viscosity.

Compared to conventional oil immersion objectives, the new 40x, 1.25 numericalaperture (NA), 0.3mm working distance objective improves resolution and reduces loss of contrast due to spherical aberration.

By reducing the mismatch between the refractive index of the specimen and that of the immersion medium into which the tip of the objective is dipped, silicone oil provides higher resolution and brightness, especially when using the microscope to image into thick samples.

Glycerol immersion optics are another option, but glycerol is sometimes not ideal because it tends to draw moisture from the air, resulting in changes in refractive index over time.

Silicone oil is very stable and does not have any of these issues; in addition, it more closely matches the refractive index (N=1.404) of intracellular components, making the new objective useful for imaging into cells during for long-term, time-lapse experiments.

The silicon objective has a correction collar, so users can correct for the spherical aberration that is present when imaging into a specimen beyond a cover slip.

Image resolution and contrast, along with fluorescence performance, are optimized and maintained by adjusting this collar.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,900+ scientific posters on ePosters
  • More than 4,200+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Photo of Water Creature Resembling a Mouse Earns First Prize
At last, a mouse that says ‘Cheese’. A photo of a curious underwater life form that bears a striking resemblance to a cartoon mouse has earned first prize in the 2011 Olympus BioScapes Digital Imaging Competition®.
Friday, November 18, 2011
Scientific News
Common Cell Transformed into Master Heart Cell
By genetically reprogramming the most common type of cell in mammalian connective tissue, researchers at the University of Wisconsin—Madison have generated master heart cells — primitive progenitors that form the developing heart.
Mitochondria Shown to Trigger Cell Ageing
An international team of scientists has for the first time shown that mitochondria, the batteries of the cells, are essential for ageing.
Cancer Cells Kill Off Healthy Neighbours
Cancer cells create space to grow by killing off surrounding healthy cells, according to UK researchers working with fruit flies.
Editing of Embryos Approved in the UK
The Human Fertilisation and Embryology Authority (HFEA) has approved a research application from the Francis Crick Institute to use new "gene editing" techniques on human embryos.
Microbes Take Their Vitamins
Scientists exploit organisms' needs in order to track 'vitamin mimics' in bacteria.
Machine Learning Uncovers Unknown Bacterial Features
Technique robustly identified characteristic gene expression patterns in response to antibiotics, low oxygen conditions.
CRISPR-Cas9 Gene Editing Advances Again
UC Berkeley researchers have made a major improvement in CRISPR-Cas9 technology that achieves an unprecedented success rate of 60 percent when replacing a short stretch of DNA with another.
Disrupting Cell’s Supply Chain Freezes Cancer Virus
When the cancer-causing Epstein-Barr virus moves into a B-cell of the human immune system, it tricks the cell into rapidly making more copies of itself, each of which will carry the virus.
Why Do Some Infections Persist?
In preparing for the possibility of an antibiotic onslaught, some bacterial cultures adopt an all-for-one/one-for-all strategy that would make a socialist proud, University of Vermont researchers have found.
ASCB: A CELLebration of Cell Biology
The last major congress of the year, ASCB is less a platform for launching new products, but one for confirming and consolidating the trends that have emerged over the past 12 months.
SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,900+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,200+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!