Corporate Banner
Satellite Banner
Cell Culture
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

JPK Reports on the Study of Structure and Dynamics of Biological Membranes

Published: Tuesday, October 15, 2013
Last Updated: Tuesday, October 15, 2013
Bookmark and Share
JPK Instruments reports on how AFM and advanced fluorescence microscopy is being applied in the study of biological membranes in the Centre de Biochimie Structurale.

The CBS includes a research group focused on single molecule physics. Dr Pierre-Emmanuel Milhiet runs a team which applies AFM and advanced fluorescence microscopies (single molecule tracking and single-molecule localization microscopy or SMLM) in the study of both structure and dynamics of biological membranes.

Speaking about his work, Dr Milhiet says "One of our aims is to decipher the molecular mechanisms involved in the lateral segregation of membrane components using artificial bilayers and intact cell membranes. Part of our activities is also to develop new methodologies and we have recently mounted a new setup combining a JPK AFM and home-made SMLM (especially PALM and STORM). The main motivation came from the fact that the lateral resolution that can be achieved with an AFM on intact cells is in the same range than that obtained by SMLM (a few tens of nanometers), making possible the precise identification of structures imaged by the tip. We are also involved in the development of high-speed AFM for imaging biological membranes as part of a collaborative effort with Professor Toshio Ando's group in Japan."

His team uses AFM because it is an outstanding tool to investigate membrane topography. Because of its vertical and lateral resolution, structure of membrane assemblies can be observed and single molecule (protein or DNA) can be delineated by the tip. The possibility to work in liquid is another tremendous advantage as compared to other structural biology techniques.

Dr Milhiet selected the JPK system because "It is a very stable machine for a stand-alone AFM allowing single protein resolution to be achieved on biological membranes. The HyperDrive™ mode is especially suitable for this purpose. Also, JPK's Tip-Assisted Optics stage can be combined with tip scanning which is very useful for compensating the drift of the sample stage which may be observed during long-term SMLM acquisition. Combining single molecule fluorescence microscopies with AFM is important as it enables us to understand that the integration of multiple methodologies over multiple length- and time-scales, from molecular to cellular levels, is necessary to tackle complex biological questions."


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,400+ scientific posters on ePosters
  • More than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.


Scientific News
Women’s Immune System Genes Operate Differently from Men’s
A new technology reveals that immune system genes switch on and off differently in women and men, and the source of that variation is not primarily in the DNA.
DNA Damage Seen in Patients Undergoing CT Scanning
Along with the burgeoning use of advanced medical imaging tests over the past decade have come rising public health concerns about possible links between low-dose radiation and cancer.
Yeast Cells Use Signaling Pathway to Modify Their Genomes
Researchers at the Babraham Institute and Cambridge Systems Biology Centre, University of Cambridge have shown that yeast can modify their genomes to take advantage of an excess of calories in the environment and attain optimal growth.
New Material Forges the Way for 'Stem Cell Factories'
Researchers have discovered the first fully synthetic substrate with potential to grow billions of stem cells. The researchcould forge the way for the creation of 'stem cell factories' - the mass production of human embryonic (pluripotent) stem cells.
New Measurements Reveal Differences Between Stem Cells for Treating Retinal Degeneration
By growing two types of stem cells in a “3-D culture” and measuring their ability to produce retinal cells, a team lead by St. Jude Children’s Research Hospital researchers has found one cell type to be better at producing retinal cells.
Researchers Identify Critical Genes Responsible for Brain Tumor Growth
After generating new brain tumor models scientists have identified the role of a family of genes underlying tumor growth in a wide spectrum of high grade brain tumors.
Growing Spinal Disc Tissue
Scientists develop new method for growing spinal disc tissue in the lab for combating chronic back pain.
A New Path Towards a Universal Flu Vaccine
New research suggests it may be possible to harness a previously unknown mechanism within the immune system to create more effective and efficient vaccines against this ever-mutating virus.
Potential New Class of Cancer Drugs
Scientists have found a way to stop cancer cell growth by targeting the Warburg Effect, a trait of cancer cell metabolism that scientists have been eager to exploit.
Human Trials of Manufactured Blood Within Two Years
The first human trials of lab-produced blood to help create better-matched blood for patients with complex blood conditions has been announced by NHS Blood and Transplant.
Skyscraper Banner

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,400+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!