Corporate Banner
Satellite Banner
Cell Culture
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

Ultra Low Adhesion Plates for Superior 3D Spheroid Cell Culture

Published: Tuesday, March 04, 2014
Last Updated: Tuesday, March 04, 2014
Bookmark and Share
Lipidure®-Coat low adhesion plates and dishes from AMSBIO.

Available from AMSBIO -Lipidure®-Coat low adhesion plates and dishes are shown in cited research to be one of the most effective tools for 3D spheroid cell culture. These new plates and dishes are available in 384-well format optimized for drug screening or other high throughput applications.

Spheroid cell culture is based on the spontaneous formation of an aggregation of cells in an environment where the interactions between cells are superior to the interactions between cells and substrate. This can be achieved by using low-adhesion cell culture conditions.

Lipidure®-Coat surfaces use MPC (2-methacryloyloxtethyl phosphorylcholine), a biocompatible synthetic polymer with the same structure as the phosphorylcholine polar bases that form cell membranes.

As a consequence of the high biocompatibility of this coating, cells will not adhere to the surface of Lipidure®-Coat plates or dishes. Different well-shapes available for 96-well plates (U, V and flat-bottom) can be used to control size, position and density of spheroids.

Studies have shown that Lipidure®-Coat promotes the formation of a single spheroid in each well of U-bottom multi-well plate, while other brands of plate lead to formation of satellite spheroids - indicating a higher degree of cell adhesion is taking place.

Formation of spheroids, tumorspheres, embryonic bodies and neurospheres using LIPIDURE® plates has already been demonstrated for cell types including ES and iPS (human and mouse), NIH3T3, pre-adipocytes, HepG2 and other cancer cell lines as well as primary neuronal cells.

For larger scale assays as well as in applications such as Western blotting or gene expression microarrays - Lipidure®-COAT dishes have produced highly reproducible, top quality results.


Further Information

Join For Free

Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 3,500+ scientific posters on ePosters
  • More than 5,000+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.

Related Content

Novel Organoid Matrix Enables Long-Term Culture of Human Hepatocytes
Organoid growth matrix to enable long-term culture of genome-stable bipotent stem cells from adult human liver.
Saturday, January 31, 2015
AMSBIO Expands US Operations
Company announces opening of a new North American headquarters in Cambridge, MA.
Wednesday, April 23, 2014
AMSBIO Publishes Comprehensive Cell Culture Handbook
Extensive 48-page handbook is intended for research scientists looking to culture cells in more physiologically relevant environments.
Friday, April 04, 2014
Webinar Addresses 3D Cell-Based Models for Regenerative Medicine
Dr Elad Katz presents new on-demand webinar.
Friday, October 25, 2013
Scientific News
New Therapeutic Target for Crohn’s Disease
A promising new target for drugs that treat IBD has been identified along with a possible biomarker for IBD severity.
Uncovering Water Bear Resilience
A protein identified in water bears can protect DNA of human cells from lethal doses of radiation damage.
Stem Cells Growing 3D Lung-in-a-Dish
Researchers have created 3D lung-like tissue from lung-derived stem cells. The tissue can be used to study lung diseases.
Reprogramming Lymph Nodes to Fight MS
Bioengineers work to reprogram lymph node function to fight multiple sclerosis.
Puttng Cells Through Their Paces
An obstacle course for human lung cells could be the answer for better testing the effectiveness of potential new drugs.
Inherited Heart Condition Breakthrough
Using stem cells, scientists have created a specific heart condition model, yeilding insights into unexpected disease mechanisms.
Genetic Tug of War Before Cells Decide Fate
Researchers report that as developing blood cells are triggered by genetic signals firing on and off, a 'tug of war' occurs.
Origin of Cultured Cells: Not Where You Think
Study shows cultured cells from decades-old cell line does not originate from the patient it was claimed to derive from.
Worms Point Way Toward Viral Strategies
Rice University wins NIH grant to study how nematodes handle gastrointestinal viruses.
Hope for Zika Treatment Found in Drug Screening
Johns Hopkins researchers join collaborative group to screen 6,000 existing drugs in hopes of finding treatments for Zika Virus infection.
SELECTBIO

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,500+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
5,000+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!