Corporate Banner
Satellite Banner
Cell Culture
Scientific Community
 
Become a Member | Sign in
Home>News>This Article
  News
Return

What Fuels Salmonella’s Invasion Strategy?

Published: Thursday, May 08, 2014
Last Updated: Tuesday, May 13, 2014
Bookmark and Share
As well as reducing the effects Salmonella can have we also need more effective ways to combat it once it's inside our bodies.

Certain strains of Salmonella bacteria such as Salmonella Typhimurium (S. Typhimurium) are among of the most common causes of food-borne gastroenteritis. Other strains of Salmonella such as S. Typhi are responsible for typhoid fever, which causes 200,000 deaths around the world each year. Ensuring food is clear of contamination, and water is clean are key to reducing the effects Salmonella can have, but we also need more effective ways to combat Salmonella once it's inside our bodies.

To address this the Institute of Food Research, strategically supported by BBSRC has been studying S.Typhimuriumbacteria to understand, not only how they transmit through the food chain, but why they are so effective and dangerous once inside us.

If we consume food or water contaminated with S. Typhimurium, the first stage of infection is to get into the cells that line our gut. These epithelial cells are adapted to defend against such attacks, but Salmonella has a wealth of strategies to overcome these and make it more virulent. It also needs these virulence genes to overcome the cells of the immune system, which it invades to move around the body. We are learning a lot about these virulence genes, but until this new study, published in the journal PLOS ONE, we didn't know how Salmonella fuelled itself for this. A source of energy and nutrition is vital, and knowing what Salmonella uses could inform new strategies to prevent infection.

To discover more about Salmonella's feeding habits, Dr Arthur Thompson and his team constructed S. Typhimurium strains lacking certain key genes in important metabolic pathways. They then examined how well these mutated strains reproduced in human epithelial cells, grown in cultures.

"We found that glucose is the major nutrient used by S.Typhimurium," said Dr Thompson. Salmonella converts glucose to pyruvate in a process called glycolysis, which also releases energy needed to fuel growth and reproduction. Knocking out one enzyme in glycolysis, and enzymes used to transport glucose into the bacteria severely reduced S. Typhimurium's ability to reproduce in epithelial cells, but didn't eradicate it completely. "This suggests that although S. Typhimurium requires glucose, it is also able to use other nutrients, and that's something we're now studying," said Dr Thompson.

This contrasts with previous findings from similar experiments on macrophage cells by the IFR team, as for successful macrophage invasion, glycolysis is absolutely essential. Macrophages are the immune cells sent to destroy Salmonella, but instead Salmonella invades the macrophages. Infected macrophages can carry Salmonella around the body causing a potentially fatal systemic infection.

"We now have a much more complete picture of the nutritional needs of Salmonella, which is important since this information may also suggest new ways to develop potential therapeutic interventions," said Dr Thompson.


Further Information
Access to this exclusive content is for Technology Networks Premium members only.

Join Technology Networks Premium for free access to:

  • Exclusive articles
  • Presentations from international conferences
  • Over 2,400+ scientific posters on ePosters
  • More than 3,700+ scientific videos on LabTube
  • 35 community eNewsletters


Sign In



Forgotten your details? Click Here
If you are not a member you can join here

*Please note: By logging into TechnologyNetworks.com you agree to accept the use of cookies. To find out more about the cookies we use and how to delete them, see our privacy policy.


Scientific News
Women’s Immune System Genes Operate Differently from Men’s
A new technology reveals that immune system genes switch on and off differently in women and men, and the source of that variation is not primarily in the DNA.
DNA Damage Seen in Patients Undergoing CT Scanning
Along with the burgeoning use of advanced medical imaging tests over the past decade have come rising public health concerns about possible links between low-dose radiation and cancer.
Yeast Cells Use Signaling Pathway to Modify Their Genomes
Researchers at the Babraham Institute and Cambridge Systems Biology Centre, University of Cambridge have shown that yeast can modify their genomes to take advantage of an excess of calories in the environment and attain optimal growth.
New Material Forges the Way for 'Stem Cell Factories'
Researchers have discovered the first fully synthetic substrate with potential to grow billions of stem cells. The researchcould forge the way for the creation of 'stem cell factories' - the mass production of human embryonic (pluripotent) stem cells.
New Measurements Reveal Differences Between Stem Cells for Treating Retinal Degeneration
By growing two types of stem cells in a “3-D culture” and measuring their ability to produce retinal cells, a team lead by St. Jude Children’s Research Hospital researchers has found one cell type to be better at producing retinal cells.
Researchers Identify Critical Genes Responsible for Brain Tumor Growth
After generating new brain tumor models scientists have identified the role of a family of genes underlying tumor growth in a wide spectrum of high grade brain tumors.
Growing Spinal Disc Tissue
Scientists develop new method for growing spinal disc tissue in the lab for combating chronic back pain.
A New Path Towards a Universal Flu Vaccine
New research suggests it may be possible to harness a previously unknown mechanism within the immune system to create more effective and efficient vaccines against this ever-mutating virus.
Potential New Class of Cancer Drugs
Scientists have found a way to stop cancer cell growth by targeting the Warburg Effect, a trait of cancer cell metabolism that scientists have been eager to exploit.
Human Trials of Manufactured Blood Within Two Years
The first human trials of lab-produced blood to help create better-matched blood for patients with complex blood conditions has been announced by NHS Blood and Transplant.
SELECTBIO

Skyscraper Banner
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
2,400+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
3,700+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FREE!