|Development of a novel xeno-free medium for feeder-free culture of human stem cells|
Annand R; Okuda Y; Inamura M
A new xeno-free medium (ReproXFTM) has been developed for feeder-free culture of stem cells.
|Novel culture medium using a small-molecule agonist of thrombopoetin receptor|
Hondo M1; Nishino T2; Inamura M1
ReproHSC medium for the culture of hematopoietic stem cells. facilitates the culture and expansion of CD34+/CD38- cells that retain their HSC properties
|A novel multi-organ microfluidic chip: on the way to the complexity of a living organism|
Timur R Samatov, Svetlana A Tonevitskaya, Natalya Pulkova, Evgeny A Tonevitsky
A novel physiologically relevant multi-organ chip is developed capable of culturing up to six different organotypic models integrated into a single microfluidic circuit.
|Continuous Collection of Stem Cells from a Human Placenta Perfusion Co-Culture|
John J.S. Cadwell & James C. Hardy
The potential for human placenta-derived cells to produce stem cells in a hollow fiber bioreactor co-culture system was investigated.
|Identification of microRNA targets using microRNA modulation techniques and gene expression arrays|
Emily M. Anderson, Maren Mayer, Kevin Sullivan, Barbara Robertson, Žaklina Strezoska, Annaleen Vermeulen, and Devin Leake
By examining the overlap of messages down-regulated by miRNA mimics and up-regulated by miRNA inhibitors, we robustly identify miRNA-regulated messages, many of which have canonical seed matches and some which are not identied by standard target prediction programs.
|Cell-Mediated Cytotoxicity Assay by High-Throughput Direct Cell Counting in Microplates using Fluorescence-Based Image Cytometry|
Leo L. Chan, Srinivas S. Somanchi, Kelsey J. Rosbach, and Dean A. Lee.
In this work, we demonstrate a novel high throughput cytotoxicity detection assay using the Celigo Imaging Cytometry method. Utilizing imaging cytometry, direct cell counting of live fluorescent target cells can be performed, which is a direct method for assessment of cytotoxicity.
|Idebenone Inhibits Cell Proliferation by Blocking of ANO1/ TMEM16A Chloride Channel in Adenocarcinoma Cells|
Idebenone significantly reduced cell proliferation and induced apoptosis in PC-3, CFPAC-1, HT-29, T-84 and Calu-3 cells having CaCCs activities. These data suggest that idebenone, an ANO1/CaCC inhibitor, has potential for use in cancer therapy.
|Non-disruptively Count and Quantify Fluorescent iPS Colonies During Secondary Reprogramming: 7 min per 6-well Plate, Dual-fluorescence Whole Well Imaging Cytometry|
SC Cribbes, S Brightwell, K Kaji
Nexcelom’s Celigo imaging cytometer has been applied to provide automated, rapid assessment of iPS reprogramming.
|Progressing 3D Spheroid Analysis into a HTS Drug Discovery Method|
Sarah Kessel, Eric Sincoff, Olivier Dery
This study demonstrates that the cell culture assay for tumorsphere size was successfully adapted to 384-well plate for a 3D spheroid assay using U-bottom low attachment 384-well and the Celigo Automated Imager for image acquisition and analysis. Moreover, the utilization of an imager provided insight on sphere and cell fate after long term drug treatment.
|Using An in‐vitro Human Airways Model to Study the Effects of e‐cigarettes on Bronchial Epithelial Cells|
Vasanthi Bathrinarayanan, P.1 Marshall, L.J.2 Leslie, L.J.1
The prime aim of our study is to use in-vitro human airway models as an effective alternative to replace animals in traditional/E-cigarette research.
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