A New Approach to Increase Yields and Improve Functionality of Recombinant Proteins
Most frequently, bacteria are grown in shake flasks or plates using commercially-available or ‘in-house’ adaptations of LB, TB or M9. Ehen supplied with a carbon source such as glucose, bacteria grow and divide rapidly at their maximum growth rate. However, as uncontrolled growth and fast aerobic metabolism deplete oxygen and glucose levels and alter the surrounding pH, bacteria soon switch over to anaerobic metabolism.
