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Rapid Screening of a Cell-based Assay for GLP-1 Receptor Using a Natural Product Library
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BioTek Instruments

The assay resembles classical radiometric ligand receptor binding, except that it uses a red-emitting labeled ligand and transfected cells. When the ligand binds to the receptor, an increase in HTRF signal may be measured. Competitive binding of non-labeled ligands or inhibitors causes a subsequent decrease.

The assay was automated using a non-contact liquid dispenser commonly used in high throughput settings.

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