Corporate Banner
Satellite Banner
Cell Culture
Scientific Community
 
Become a Member | Sign in
Home>Resources>Application Notes>This Application Note
  Application Notes
Scientific News
Emerging Model of Cancer
Cancer acts cooperatively, making individual decisions but acting in unison; this insight is being used to create a computer model of cancer.
Characterizing the Mouse Gut Microbiome
Study establishes the first public collection of bacteria from the intestine of mice.
How Cloud Connectivity Can Combat the Reproducibility Crisis
This infographic explains the reproducibility crisis, and how cloud connectivity can help overcome this problem.
Stem Cells Grown On Scaffold Mimic Hip Joint Cartilage
Adult fat-derived stem cells grown on a 3-D scaffold that mimicked a hip joint surface formed cartilage and maintained the correct shape.
'Fixing' Blood Vessel Cells To Diagnose Blood Clotting Disorders
A novel microfluidic device detects how endothelial cells that line vessels contribute to hemostasis.
Using Animal Embryos Containing Human Cells
With recent advances in stem cell and gene editing technologies, an increasing number of researchers are interested in growing human tissues and organs in animals by introducing pluripotent human cells into early animal embryos.
Engineering Cells to Speed Tissue Repair
Kelly Schultz studies how cells remodel their microenvironment - a crucial step toward engineering cells to move through synthetic material for faster tissue regeneration.
Cancer Cells Migrate Towards Oxygen
Bioengineers report results showing sarcoma cells in mice finding pathways toward higher concentrations of oxygen
Biopharmaceuticals on Demand
Portable production system would use microbes for manufacturing small amounts of vaccines and therapeutics.
Singapore Scientists Grow Mini Human Brains
Mini midbrains provide next generation platforms to investigate human brain biology, diseases and therapeutics
Scroll Up
Scroll Down

3D cell culture: a realistic and reproducible collagen-based environment
Bookmark and Share

Tecan Group

In drug discovery and many other areas of research, cell culture using primary cells of human or animal origin is often desirable, or even essential. TAP Biosystems has developed a novel 3D cell culture technique, RAFT (Real Architecture for 3D Tissue), which uses the most abundant matrix protein in the body: type I collagen. 

This technology allows researchers to culture cell types of their choice, including primary cells, in an in vivo like environment that is essentially a hydrogel with tissue-like properties. Uniquely, the RAFT process increases the collagen concentration to physiological levels rapidly and reproducibly, while maintaining high cell viability. 

Tecan and TAP Biosystems have developed an automated process for the preparation of RAFT cell cultures, aiding mixing procedures and enhancing reproducibility while maintaining excellent cell viability and reliability. Implementation on the Freedom EVO system, with its modular automation capabilities, increases experimental versatility and analytical flexibility. 

This application note presents cell viability and proliferation data for primary neonatal human dermal fibroblasts (Life Technologies) obtained over an 11-day culture period after seeding.


Further Information

Related Content

Investigating Protein Targets and Cellular Pathways in Yeasts
Scientists at the Donnelly Centre for Cellular and Biomolecular Research have chosen generations of Tecan microplate readers to monitor the effects of environmental or drug perturbation on molecularly barcoded yeasts.
Monday, October 07, 2013
Tecan and Reinnervate Team up for Automation of 3D Cell Culture
Alvetex Scaffold for the automation of 3D cell culture.
Tuesday, June 26, 2012
Skyscraper Banner

SELECTBIO Market Reports
Go to LabTube
Go to eposters
 
Access to the latest scientific news
Exclusive articles
Upload and share your posters on ePosters
Latest presentations and webinars
View a library of 1,800+ scientific and medical posters
3,300+ scientific and medical posters
A library of 2,500+ scientific videos on LabTube
4,900+ scientific videos
Close
Premium CrownJOIN TECHNOLOGY NETWORKS PREMIUM FOR FREE!