Corporate Banner
Satellite Banner
Technology Networks Header
Friday, August 22, 2014
Technology Networks
 
Register | Sign in
Home Page > Videos > Detection and Expression Profiling of Tumor Initiating Cells (TIC) Among the Peripherally Circulating Epithelial Tumor Cells from Patients with Breast Cancer
  Videos

Return

Detection and Expression Profiling of Tumor Initiating Cells (TIC) Among the Peripherally Circulating Epithelial Tumor Cells from Patients with Breast Cancer
SELECTBIO

The detection of tumor cells circulating in the peripheral blood of patients with breast cancer is a sign that cells have been able to leave the primary tumor and survive in the circulation. However, in order to form metastases they require additional properties such as the ability to adhere, self renew and grow. Here we present data that a variable fraction among the circulating tumor cells detected by the maintrac® approach express mRNA of the stem cell gene nanog and of the adhesion molecule vimentin and are capable of forming tumor spheres a property ascribed to TIC. Between 10 to 50 circulating epithelial antigen positive cells detected by the maintrac® approach were selected randomly from each of 20 patients with breast cancer before and after surgery and isolated using automated capillary aspiration and deposited individually onto slides for expression profiling. In addition, the circulating tumor cells were cultured without isolation among the white blood cells from five patients with breast cancer in different stages of disease using culture methods favoring growth of epithelial cells. Although no EpCAM mRNA positive cells expressing stem cell genes or the adhesion molecule vimentin were detected before surgery, 10 to 20% of the cells were found to be positive for mRNA of these genes after surgery. Most surprisingly, it was possible to grow tumor spheres rom these circulating cells without previous isolation in all patients in a fraction comprising between 1‰ of the epithelial antigen positive cells in patients with newly diagnosed tumor up to 10% in a patient with advanced breast cancer. We show here, that among the peripherally circulating tumor cells a variable fraction is able to express stem cell and adhesion properties and can be grown into tumor spheres, a property ascribed to cells capable of initiating tumors and metastases.

Request more information
Company product page



For access to this article, enter your email address to instantly recieve a Password Reset link.

Please enter your email address below:

Existing users please Sign In here. Don't have an account? Register Here for free access.

Don't have an account? | Register Here

Scientific News
Assessing Anticancer Drugs Efficacy by Amino Acid Metabolomics
Researchers aimed to develop a method that determines the therapeutic efficacy of anticancer drugs based on the changes in amino acid metabolism at the cellular level.
Apple Derived Cellulose Scaffolds for 3D Mammalian Cell Culture
Researchers demonstrate that cellulose scaffolds are a potentially useful biomaterial that can be successfully employed for in vitro 3D cell culture.
Advanced Cell Culture Techniques for Cancer Drug Discovery
This review focuses on the culturing of cell lines representative of solid cancers in advanced cell culture conditions.
Silicon Substrate as a Novel Cell Culture Device for Myoblast Cells
Researchers describe the use of a commercially available monocrystaline silicon PV device to be used as substrate for culturing of C2C12 mammalian cells.
Ptychographic Microscope for Three-Dimensional Imaging
3D ptychographic method used to visualize 3D specimens up to 34 tomographic sections in depth.
3D Cell Culture System to Study Tumour-Stroma Interactions
The model allows investigation of tumour-stroma interactions, addressing the importance of having a more in vivo like cell culture model.
What Fuels Salmonella’s Invasion Strategy?
As well as reducing the effects Salmonella can have we also need more effective ways to combat it once it's inside our bodies.
InSphero Publishes 3D Tumor-Stroma Model for Non-Small Cell Lung Cancer
PLOS One article delivers promising co-culture method to mimic tumor microenvironment, demonstrating potential for biomarker and drug discovery.
How a Silly Putty Ingredient Could Advance Stem Cell Therapies
The sponginess of the environment where human embryonic stem cells are growing affects the type of specialized cells they eventually become.
AMSBIO Publishes Comprehensive Cell Culture Handbook
Extensive 48-page handbook is intended for research scientists looking to culture cells in more physiologically relevant environments.
Scroll Up
Scroll Down
Skyscraper Banner
Skyscraper Banner
Follow TechNetcom1 on Twitter
Technology Networks Ltd. on LinkedIn
Go to LabTube.tv