Corporate Banner
Satellite Banner
Technology Networks Header
Monday, December 22, 2014
Technology Networks
 
Register | Sign in
Home Page > Videos > Detection and Expression Profiling of Tumor Initiating Cells (TIC) Among the Peripherally Circulating Epithelial Tumor Cells from Patients with Breast Cancer
  Videos

Return

Detection and Expression Profiling of Tumor Initiating Cells (TIC) Among the Peripherally Circulating Epithelial Tumor Cells from Patients with Breast Cancer
SELECTBIO

The detection of tumor cells circulating in the peripheral blood of patients with breast cancer is a sign that cells have been able to leave the primary tumor and survive in the circulation. However, in order to form metastases they require additional properties such as the ability to adhere, self renew and grow. Here we present data that a variable fraction among the circulating tumor cells detected by the maintrac® approach express mRNA of the stem cell gene nanog and of the adhesion molecule vimentin and are capable of forming tumor spheres a property ascribed to TIC. Between 10 to 50 circulating epithelial antigen positive cells detected by the maintrac® approach were selected randomly from each of 20 patients with breast cancer before and after surgery and isolated using automated capillary aspiration and deposited individually onto slides for expression profiling. In addition, the circulating tumor cells were cultured without isolation among the white blood cells from five patients with breast cancer in different stages of disease using culture methods favoring growth of epithelial cells. Although no EpCAM mRNA positive cells expressing stem cell genes or the adhesion molecule vimentin were detected before surgery, 10 to 20% of the cells were found to be positive for mRNA of these genes after surgery. Most surprisingly, it was possible to grow tumor spheres rom these circulating cells without previous isolation in all patients in a fraction comprising between 1‰ of the epithelial antigen positive cells in patients with newly diagnosed tumor up to 10% in a patient with advanced breast cancer. We show here, that among the peripherally circulating tumor cells a variable fraction is able to express stem cell and adhesion properties and can be grown into tumor spheres, a property ascribed to cells capable of initiating tumors and metastases.

Request more information
Company product page



For access to this article, enter your email address to instantly recieve a Password Reset link.

Please enter your email address below:

Existing users please Sign In here. Don't have an account? Register Here for free access.

Don't have an account? | Register Here

Scientific News
Tailor-Made Cancer Treatments? New Cell Culture Technique Paves The Way
Technique grew cells from 73% of patients in the study, more than three times as effective as previous methods.
Recreating Stem Cells From Deceased Patients to Study Present-Day Illnesses
Cedars-Sinai team remakes brain and gut stem cells in a dish to test potential therapies for debilitating and fatal diseases.
Protein Key To The Development Of Blood Stem Cells Identified
Understanding the self-replication mechanisms is critical for improving stem cell therapies for blood-related diseases and cancers.
Pain In A Dish
Turning skin cells into pain-sensing neurons.
Direct Drug Screening Of Biopsies Could Overcome Resistance
Combining genetic with pharmacologic screening of tumors may enable truly individualized treatment regimens.
Microtubes Create Cozy Space For Neurons To Grow
Illinois researchers developed a platform to grow and study neuron cells using tiny rolled microtubes.
Norovirus Grown In Human Cells
UF researchers have grown norovirus in a cell culture dish.
Eppendorf Award for Young European Investigators 2015: Call for Entries!
Eppendorf Award celebrates 20 years in 2015.
Heart Drug May Help Treat ALS, Mouse Study Shows
Scientists blocked the enzyme in ALS astrocytes using digoxin, a medication used in the treatment of heart failure.
Cell Division, Minus the Cells
Scientists assemble basic biological phenomenon from scratch
Scroll Up
Scroll Down

SELECTBIO Market Reports
LabTube - Videos for the Scientific Community
eposters - The Online Journal of Scientific Posters
Follow TechNetcom1 on Twitter
Technology Networks Ltd. on LinkedIn